PJ 34 hydrochloride

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Cat.No. 3255 - PJ 34 hydrochloride | C17H17N3O2.HCl | CAS No. 344458-15-7
Description: Potent PARP inhibitor
Chemical Name: N-(5,6-Dihydro-6-oxo-2-phenanthridinyl)-2-acetamide hydrochloride
Purity: ≥98% (HPLC)
Datasheet
Citations (1)
Literature

Biological Activity

Potent inhibitor of poly(ADP-ribose) polymerase (PARP) (EC50 = 20 nM). ~1000-fold more potent than 3-Aminobenzamide (Cat. No. 0788). Protects primary neuronal cells from oxygen-glucose deprivation in vitro and reduces infarct size following focal cerebral ischemia in vivo. Displays protective effects against cisplatin-induced kidney injury. Also displays activity at Pim-1 and Pim-2 kinases at higher concentrations (IC50 values are 3.7 and 16 μM respectively).

Compound Libraries

PJ 34 hydrochloride is also offered as part of the Tocriscreen Plus and Tocriscreen Epigenetics Toolbox. Find out more about compound libraries available from Tocris.

Technical Data

M. Wt 331.8
Formula C17H17N3O2.HCl
Storage Desiccate at RT
Purity ≥98% (HPLC)
CAS Number 344458-15-7
PubChem ID 16760621
InChI Key RURAZZMDMNRXMI-UHFFFAOYSA-N
Smiles Cl.CN(C)CC(=O)NC1=CC2=C(NC(=O)C3=CC=CC=C23)C=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 33.18 100
water 33.18 100

Preparing Stock Solutions

The following data is based on the product molecular weight 331.8. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.01 mL 15.07 mL 30.14 mL
5 mM 0.6 mL 3.01 mL 6.03 mL
10 mM 0.3 mL 1.51 mL 3.01 mL
50 mM 0.06 mL 0.3 mL 0.6 mL

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Product Datasheets

Certificate of Analysis / Product Datasheet
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Safety Datasheet

References

References are publications that support the products' biological activity.

Abdelkarim et al (2001) Protective effects of PJ34, a novel, potent inhibitor of poly(ADP-ribose) polymerase (PARP) in in vitro and in vivo models of stroke. Int.J.Mol.Med. 7 255 PMID: 11179503

Gambi et al (2008) Poly(ADPR)polymerase inhibition and apoptosis induction in cDDP-treated human carcinoma cell lines. Biochem.Pharmacol. 75 2356 PMID: 18468580

Kim et al (2012) Poly(ADP-ribose) polymerase 1 activation is required for cisplatin nephrotoxicity. Kidney Int. 82 193 PMID: 22437413

Antolin et al (2012) Identification of Pim kinases as novel targets for PJ34 with confounding effects in PARP biology. ACS Chem.Biol. 7 1962 PMID: 23025350


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1 Citation for PJ 34 hydrochloride

Citations are publications that use Tocris products. Selected citations for PJ 34 hydrochloride include:

Kirby et al (2012) Structure of human tankyrase 1 in complex with small-molecule inhibitors PJ34 and XAV939. Acta Crystallogr Sect F Struct Biol Cryst Commun 68 115 PMID: 22297980


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