Peroxy Orange 1

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Description: Fluorescent hydrogen peroxide indicator
Alternative Names: PO1
Chemical Name: 2',3',6',7'-Tetrahydro-12'-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-spiro[isobenzofuran-1(3H),9'-[1H,5H,9H]xantheno[2,3,4-ij]quinolizin]-3-one
Citations (2)
Literature (1)

Biological Activity for Peroxy Orange 1

Peroxy Orange 1 is a fluorescent probe for imaging hydrogen peroxide (H2O2); cell permeable. Displays an orange intracellular fluorescence in response to H2O2 signals produced in RAW 264.7 macrophages and A431 cells during immune response and growth factor stimulation, respectively. Excitation wavelength 543 nm, emission between 545 and 750 nm.

Licensing Information

Sold under license from the Regents of the University of California

Technical Data for Peroxy Orange 1

M. Wt 521.41
Formula C32H32BNO5
Storage Store at -20°C
CAS Number 1199576-10-7
PubChem ID 44546671
Smiles O=C(O1)C2=C(C=CC=C2)C31C4=CC5=C6C(CCCN6CCC5)=C4OC7=C3C=CC(B8OC(C)(C)C(C)(C)O8)=C7

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Peroxy Orange 1

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 5.21 10

Preparing Stock Solutions for Peroxy Orange 1

The following data is based on the product molecular weight 521.41. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.1 mM 19.18 mL 95.89 mL 191.79 mL
0.5 mM 3.84 mL 19.18 mL 38.36 mL
1 mM 1.92 mL 9.59 mL 19.18 mL
5 mM 0.38 mL 1.92 mL 3.84 mL

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Product Datasheets for Peroxy Orange 1

Certificate of Analysis / Product Datasheet
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References for Peroxy Orange 1

References are publications that support the biological activity of the product.

Dickinson et al (2010) A palette of fluorescent probes with varying emission colors for imaging hydrogen peroxide signaling in living cells. J.Am.Chem.Soc. 132 5906 PMID: 20361787

Dickinson et al (2011) Chemistry and biology of reactive oxygen species in signaling or stress responses. Nat.Chem.Biol. 7 504 PMID: 21769097

Woolley et al (2012) H2O2 production downstream of FLT3 is mediated by p22phox in the endoplasmic reticulum and is required for STAT5 signalling. PLoS ONE 7 PMID: 22807997

If you know of a relevant reference for Peroxy Orange 1, please let us know.

Keywords: Peroxy Orange 1, Peroxy Orange 1 supplier, Fluorescent, probe, hydrogen, peroxide, H2O2, po1, po-1, peroxyorange, orange, immune, growth, factor, excitation, wavelength, emission, PO1, Cell, Indicators, and, Sensors, 4944, Tocris Bioscience

2 Citations for Peroxy Orange 1

Citations are publications that use Tocris products. Selected citations for Peroxy Orange 1 include:

Young Taek et al (2016) A single gene of a commensal microbe affects host susceptibility to enteric infection. Nat Commun 7 11606 PMID: 27173141

Jeong et al (2017) Prdx1 (peroxiredoxin 1) deficiency reduces cholesterol efflux via impaired macrophage lipophagic flux. Autophagy 14 120 PMID: 28605287

Do you know of a great paper that uses Peroxy Orange 1 from Tocris? Please let us know.

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Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

Fluorescent Dyes and Probes Research Product Guide

Fluorescent Dyes and Probes Research Product Guide

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