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Peroxy Orange 1
Fluorescent probe for imaging hydrogen peroxide (H2O2); cell permeable. Displays an orange intracellular fluorescence in response to H2O2 signals produced in RAW 264.7 macrophages and A431 cells during immune response and growth factor stimulation, respectively. Excitation wavelength 543 nm, emission between 545 and 750 nm.
Sold under license from the Regents of the University of California
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions
The following data is based on the product molecular weight 521.41. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|0.1 mM||19.18 mL||95.89 mL||191.79 mL|
|0.5 mM||3.84 mL||19.18 mL||38.36 mL|
|1 mM||1.92 mL||9.59 mL||19.18 mL|
|5 mM||0.38 mL||1.92 mL||3.84 mL|
References are publications that support the biological activity of the product.
Dickinson et al (2010) A palette of fluorescent probes with varying emission colors for imaging hydrogen peroxide signaling in living cells. J.Am.Chem.Soc. 132 5906 PMID: 20361787
Dickinson et al (2011) Chemistry and biology of reactive oxygen species in signaling or stress responses. Nat.Chem.Biol. 7 504 PMID: 21769097
Woolley et al (2012) H2O2 production downstream of FLT3 is mediated by p22phox in the endoplasmic reticulum and is required for STAT5 signalling. PLoS ONE 7 PMID: 22807997
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1 Citation for Peroxy Orange 1
Citations are publications that use Tocris products. Selected citations for Peroxy Orange 1 include:
Jeong et al (2017) Prdx1 (peroxiredoxin 1) deficiency reduces cholesterol efflux via impaired macrophage lipophagic flux. Autophagy 14 120 PMID: 28605287
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Literature in this Area
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