Peroxy Orange 1

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Cat.No. 4944 - Peroxy Orange 1 | C32H32BNO5 | CAS No. 1199576-10-7
Description: Fluorescent hydrogen peroxide indicator
Alternative Names: PO1
Chemical Name: 2',3',6',7'-Tetrahydro-12'-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-spiro[isobenzofuran-1(3H),9'-[1H,5H,9H]xantheno[2,3,4-ij]quinolizin]-3-one
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Literature

Biological Activity

Fluorescent probe for imaging hydrogen peroxide (H2O2); cell permeable. Displays an orange intracellular fluorescence in response to H2O2 signals produced in RAW 264.7 macrophages and A431 cells during immune response and growth factor stimulation, respectively. Excitation wavelength 543 nm, emission between 545 and 750 nm.

Licensing Information

Sold under license from the Regents of the University of California

Technical Data

M. Wt 521.41
Formula C32H32BNO5
Storage Store at -20°C
CAS Number 1199576-10-7
PubChem ID 44546671
InChI Key KNTNXZHHCPNNGB-UHFFFAOYSA-N
Smiles O=C(O1)C2=C(C=CC=C2)C31C4=CC5=C6C(CCCN6CCC5)=C4OC7=C3C=CC(B8OC(C)(C)C(C)(C)O8)=C7

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 5.21 10

Preparing Stock Solutions

The following data is based on the product molecular weight 521.41. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.1 mM 19.18 mL 95.89 mL 191.79 mL
0.5 mM 3.84 mL 19.18 mL 38.36 mL
1 mM 1.92 mL 9.59 mL 19.18 mL
5 mM 0.38 mL 1.92 mL 3.84 mL

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References

References are publications that support the biological activity of the product.

Dickinson et al (2010) A palette of fluorescent probes with varying emission colors for imaging hydrogen peroxide signaling in living cells. J.Am.Chem.Soc. 132 5906 PMID: 20361787

Dickinson et al (2011) Chemistry and biology of reactive oxygen species in signaling or stress responses. Nat.Chem.Biol. 7 504 PMID: 21769097

Woolley et al (2012) H2O2 production downstream of FLT3 is mediated by p22phox in the endoplasmic reticulum and is required for STAT5 signalling. PLoS ONE 7 PMID: 22807997


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