Pepstatin A Janelia Fluor® 526

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Cat.No. 7314 - Pepstatin A Janelia Fluor 526 | C67H91F6N9O12.CF3CO2H | CAS No. 2410614-07-0
Description: Fluorogenic green-emitting lysosome tracker and stain
Alternative Names: Pepstatin A JF526
Chemical Name: 2-(3-(3,3-Difluoroazetidin-1-ium-1-ylidene)-6-(3,3-difluoroazetidin-1-yl)-2,7-difluoro-3H-xanthen-9-yl)-4-(((6S,9S,12S,13S,17S,20S,21S)-13,21-dihydroxy-12,20-diisobutyl-6,9-diisopropyl-2,17-dimethyl-4,7,10,15,18,23-hexaoxo-5,8,11,16,19,24-hexaazatriacontan-30-yl)carbamoyl)benzoate trifluoroacetate
Purity: ≥90% (HPLC)
Reviews (1)

Biological Activity for Pepstatin A Janelia Fluor® 526

Pepstatin A Janelia Fluor® 526 is a fluorogenic green-emitting lysosome tracker and stain. No wash step is required when used for multicolor microscopy experiments. Pepstatin A Janelia Fluor® 526 can be used for live cell imaging, in confocal microscopy and super-resolution microscopy techniques including 3D-SIM (structured illumination microscopy) and STED, as well as two-color lattice light-sheet microscopy (lls). Excitation maximum = 530 nm, emission maximum = 549 nm.

To measure the absorbance spectrum of this dye we recommend the following solvent: TFE plus 0.1% TFA.

Licensing Information

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Optical Data for Pepstatin A Janelia Fluor® 526

Pepstatin A Janelia Fluor<sup>®</sup> 526 Dye Spectra
Emission Color Green
λabs 530 nm
λem 549 nm
Extinction Coefficient (ε) 118,000 M-1cm-1
Quantum Yield (φ) 0.87
Application confocal microscopy, STED, 3D-SIM, lattice light-sheet microscopy
Alternative For LysotrackerTM- Lysotracker is a registered trademark of Thermo Fisher

Technical Data for Pepstatin A Janelia Fluor® 526

M. Wt 1442.53
Formula C67H91F6N9O12.CF3CO2H
Storage Store at -20°C
Purity ≥90% (HPLC)
CAS Number 2410614-07-0
Smiles OC(C(F)(F)F)=O.CC(C[C@@H]([C@H](CC(NCCCCCCNC(C1=CC(C2=C3C=C(/C(C=C3OC4=C2C=C(C(N5CC(F)(C5)F)=C4)F)=[N+]6CC(F)(C\6)F)F)=C(C([O-])=O)C=C1)=O)=O)O)NC([C@@H](NC(C[C@@H]([C@@H](NC([C@H](C(C)C)NC([C@H](C(C)C)NC(CC(C)C)=O)=O)=O)CC(C)C)O)=O)C)=O)C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Pepstatin A Janelia Fluor® 526

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 14.43 10

Preparing Stock Solutions for Pepstatin A Janelia Fluor® 526

The following data is based on the product molecular weight 1442.53. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.1 mM 6.93 mL 34.66 mL 69.32 mL
0.5 mM 1.39 mL 6.93 mL 13.86 mL
1 mM 0.69 mL 3.47 mL 6.93 mL
5 mM 0.14 mL 0.69 mL 1.39 mL

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

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Product Datasheets for Pepstatin A Janelia Fluor® 526

References for Pepstatin A Janelia Fluor® 526

References are publications that support the biological activity of the product.

Zheng et al (2019) Rational design of fluorogenic and spontaneously blinking labels for super-resolution imaging. ACS Cent.Sci. 5 1602 PMID: 31572787

If you know of a relevant reference for Pepstatin A Janelia Fluor® 526, please let us know.

Keywords: Pepstatin A Janelia Fluor 526, Pepstatin A Janelia Fluor 526 supplier, Fluorogenic, green, emitting, lysosomes, lysosomal, probes, stains, dyes, tracker, staining, Pepstatin, A, JF526, Fluorescent, Lysosome, Probes, 7314, Tocris Bioscience

Citations for Pepstatin A Janelia Fluor® 526

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Specific dye.
By Karthik Krishnamurthy on 08/03/2021
Assay Type: In Vitro
Species: Rat
Cell Line/Tissue: astrocytes

labeling of lysosomes in astrocytes under basal conditions

review image