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Pepstatin A Janelia Fluor® 526 New
Biological Activity for Pepstatin A Janelia Fluor® 526
Pepstatin A Janelia Fluor® 526 is a fluorogenic green-emitting lysosome tracker and stain. No wash step is required when used for multicolor microscopy experiments. Pepstatin A Janelia Fluor® 526 can be used for live cell imaging, in confocal microscopy and super-resolution microscopy techniques including 3D-SIM (structured illumination microscopy) and STED, as well as two-color lattice light-sheet microscopy (lls). Excitation maximum = 530 nm, emission maximum = 549 nm.
To measure the absorbance spectrum of this dye we recommend the following solvent: TFE plus 0.1% TFA.
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus
Optical Data for Pepstatin A Janelia Fluor® 526
Technical Data for Pepstatin A Janelia Fluor® 526
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Pepstatin A Janelia Fluor® 526
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions for Pepstatin A Janelia Fluor® 526
The following data is based on the product molecular weight 1442.53. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|0.1 mM||6.93 mL||34.66 mL||69.32 mL|
|0.5 mM||1.39 mL||6.93 mL||13.86 mL|
|1 mM||0.69 mL||3.47 mL||6.93 mL|
|5 mM||0.14 mL||0.69 mL||1.39 mL|
Product Datasheets for Pepstatin A Janelia Fluor® 526
References for Pepstatin A Janelia Fluor® 526
References are publications that support the biological activity of the product.
Zheng et al (2019) Rational design of fluorogenic and spontaneously blinking labels for super-resolution imaging. ACS Cent.Sci. 5 1602 PMID: 31572787
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Citations for Pepstatin A Janelia Fluor® 526
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