You can now submit reviews for your favorite Tocris products. Your review will help other researchers decide on the best products for their research. Why not submit a review today?!Submit Review
PEI STAR™ transfection reagent
Biological Activity for PEI STAR™ transfection reagent
PEI STAR™ is a chemically-defined, high-performance polyethylenimine (PEI) transfection reagent for cost-effective, affordable and scalable transient gene expression. PEI is a synthetic polymer with an exceptionally high positive charge density in pH-neutral solutions. Positively charged PEI binds strongly to negatively charged DNA and imparts a net cationic charge, allowing the DNA to enter cells. PEI is a non-viral vector commonly used to transfect HEK293 and CHO cells. Applications include production of recombinant proteins, antibodies and viruses.
PEI STAR is a trademark of Bio-Techne Corp.
Application of PEI STAR™ transfection reagent. PEI STAR™ performance data comparison (HEK293): HEK 293 - 20 mL cultures containing HEK293 suspensions were transfected with a CMV-SEAP plasmid at optimized PEI/DNA ratios using either PEI STAR™ (3:1) or leading competitor PEI. SEAP expression levels were quantified 5 days post-transfection using phosphatase reporter dye and UV/Vis absorbance.
Application of PEI STAR™ transfection reagent. PEI STAR™ performance data comparison (CHO): CHO - 20 mL cultures containing CHO suspensions were transfected with a CMV-SEAP plasmid at optimized PEI/DNA ratios using either PEI STAR™ (5:1) or leading competitor PEI. SEAP expression levels were quantified 5 days post-transfection using phosphatase reporter dye and UV/Vis absorbance.
Protocols for PEI STAR™ transfection reagent
Please see the links below for protocols relating to the use of PEI STAR™
|Protocol Name||Cell Type|
|PEI STAR™ Transfection Reagent preparation|
|Gene Expression in Adherent HEK293 cells||Adherent|
|rAAV Production in Adherent HEK293 Cells||Adherent|
|Lentivirus Production in Adherent HEK293T Cells||Adherent|
|Gene Expression in HEK293 Cell Suspensions||Suspension|
|rAAV Production in Suspension HEK293 Cells||Suspension|
|Gene Expression in CHO Suspensions||Suspension|
Technical Data for PEI STAR™ transfection reagent
|Storage||Store at RT|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Product Datasheets for PEI STAR™ transfection reagent
References for PEI STAR™ transfection reagent
References are publications that support the biological activity of the product.
Boussif et al (1995) A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: polyethylenimine. Proc.Natl.Acad.Sci.U.S.A 92 7297 PMID: 7638184
Longo et al (2013) Transient mammalian cell transfection with polyethylenimine (PEI). Methods Enzymol. 529 227 PMID: 24011049
Huang et al (2013) AAV2 production with optimized N/P ratio and PEI-mediated transfection results in low toxicity and high titer for in vitro and in vivo applications. J.Virol.Methods 193 270 PMID: 23791963
Trivedi et al (2021) Comparison of highly pure rAAV9 vector stocks produced in suspension by PEI transfection or HSV infection reveals striking quantitative and qualitative differences. Mol.Ther.Methods Clin.Dev. 24 154 PMID: 35071688
Han et al (2021) Aberrant role of pyruvate kinase M2 in the regulation of gamma-secretase and memory deficits in Alzheimer's disease. Cell Rep. 37 110102 PMID: 34879266
If you know of a relevant reference for PEI STAR™ transfection reagent, please let us know.
View Related Products by Target
Keywords: PEI STAR™ transfection reagent, PEI STAR™ transfection reagent supplier, polyethylenimine, PEI, transfection, reagent, non-viral, vector, HEK293, CHO, transient, gene, expression, Biochemicals, and, Molecular, Biology, 7854, Tocris Bioscience
Citations for PEI STAR™ transfection reagent
Citations are publications that use Tocris products.
Currently there are no citations for PEI STAR™ transfection reagent. Do you know of a great paper that uses PEI STAR™ transfection reagent from Tocris? Please let us know.
Reviews for PEI STAR™ transfection reagent
There are currently no reviews for this product. Be the first to review PEI STAR™ transfection reagent and earn rewards!
Have you used PEI STAR™ transfection reagent?
Submit a review and receive an Amazon gift card.
$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Protocols for PEI STAR™ transfection reagent
The following protocols feature additional information for the use of PEI STAR™ transfection reagent (Cat. No. 7854).