PEI STAR™ transfection reagent

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Description: Polyethylenimine (PEI) transfection reagent
Chemical Name: Poly[imino(1,2-ethanediyl)] hydrochloride
Datasheet
Citations
Reviews
Protocols (7)

Biological Activity for PEI STAR™ transfection reagent

PEI STAR™ is a high-performance polyethylenimine (PEI) transfection reagent for affordable and scalable transient gene expression. PEI is a synthetic polymer with an exceptionally high positive charge density in pH-neutral solutions. Positively charged PEI binds strongly to negatively charged DNA and imparts a net cationic charge, allowing the DNA to enter cells. PEI is a non-viral vector commonly used to transfect HEK293 and CHO cells. Applications include production of recombinant proteins, antibodies and viruses.

PEI STAR is a trademark of Bio-Techne Corp.

Protocols for PEI STAR™ transfection reagent

Please see the links below for protocols relating to the use of PEI STAR

Protocol Name Cell Type
PEI STAR Transfection Reagent preparation  
Gene Expression in Adherent HEK293 cells Adherent
rAAV Production in Adherent HEK293 Cells Adherent
Lentivirus Production in Adherent HEK293T Cells Adherent
Gene Expression in HEK293 Cell Suspensions Suspension
rAAV Production in Suspension HEK293 Cells Suspension
Gene Expression in CHO Suspensions Suspension
PEI STAR transfection efficiency in HEK293 cells comparison

PEI STAR™ performance data comparison (HEK293): HEK 293 - 20 mL cultures containing HEK293 suspensions were transfected with a CMV-SEAP plasmid at optimized PEI/DNA ratios using either PEI STAR™ (3:1) or leading competitor PEI. SEAP expression levels were quantified 5 days post-transfection using phosphatase reporter dye and UV/Vis absorbance.

PEI STAR transfection efficiency in CHO cells comparison

PEI STAR™ performance data comparison (CHO): CHO - 20 mL cultures containing CHO suspensions were transfected with a CMV-SEAP plasmid at optimized PEI/DNA ratios using either PEI STAR™ (5:1) or leading competitor PEI. SEAP expression levels were quantified 5 days post-transfection using phosphatase reporter dye and UV/Vis absorbance.

Technical Data for PEI STAR™ transfection reagent

Storage Store at RT
CAS Number 49553-93-7

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Product Datasheets for PEI STAR™ transfection reagent

Certificate of Analysis / Product Datasheet
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References for PEI STAR™ transfection reagent

References are publications that support the biological activity of the product.

Boussif et al (1995) A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: polyethylenimine. Proc.Natl.Acad.Sci.U.S.A 92 7297 PMID: 7638184

Longo et al (2013) Transient mammalian cell transfection with polyethylenimine (PEI). Methods Enzymol. 529 227 PMID: 24011049

Huang et al (2013) AAV2 production with optimized N/P ratio and PEI-mediated transfection results in low toxicity and high titer for in vitro and in vivo applications. J.Virol.Methods 193 270 PMID: 23791963

Trivedi et al (2021) Comparison of highly pure rAAV9 vector stocks produced in suspension by PEI transfection or HSV infection reveals striking quantitative and qualitative differences. Mol.Ther.Methods Clin.Dev. 24 154 PMID: 35071688

Han et al (2021) Aberrant role of pyruvate kinase M2 in the regulation of gamma-secretase and memory deficits in Alzheimer's disease. Cell Rep. 37 110102 PMID: 34879266


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Citations for PEI STAR™ transfection reagent

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