PA Janelia Fluor® 646, SE

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Cat.No. 6150 - PA Janelia Fluor 646, SE | C34H31N5O5Si
Description: Photoactivatable cell permeable fluorescent dye; supplied as NHS ester
Chemical Name: 2,5-Dioxo-1-pyrrolidinyl 3,7-di-1-azetidinyl-2'-diazo-5,5-dimethyl-2',3'-dihydro-5H-3'-oxospiro[dibenzo[b,e]siline-10,1'-indene]-6'-carboxylate
Datasheet
Citations
Reviews (1)
Protocols (1)
Literature

Biological Activity

Photoactivatable fluorescent dye; supplied as an NHS ester for coupling to primary amine groups. Non-fluorescent until activated at 365 nm. Compatible with self-labeling tag systems (e.g. HaloTag® and SNAPtag® ). Suitable for single molecule tracking and super resolution microscopy in live cells, specifically live cell sptPALM and fixed cell PALM. Can be multiplexed with PA Janelia Fluor® 549, SE (Cat. No. 6149) to perform two-colour sptPALM in live cells with activation at 405nm. Cell permeable. Excitation maximum = 649 - 651 nm; emission maximum = 663 - 665 nm. Upon protein conjugation the efficiencies of photoconversion improve substantially. Low background staining.

HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.

Licensing Information

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Technical Data

M. Wt 617.74
Formula C34H31N5O5Si
Storage Store at -20°C
PubChem ID 127021042
InChI Key XVIATAHKOZWKHH-UHFFFAOYSA-N
Smiles O=C(ON1C(CCC1=O)=O)C2=CC=C(C(C3=[N+]=[N-])=O)C(C43C5=CC=C(N6CCC6)C=C5[Si](C)(C)C7=CC(N8CCC8)=CC=C47)=C2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 12.35 20

Preparing Stock Solutions

The following data is based on the product molecular weight 617.74. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.62 mL 8.09 mL 16.19 mL
5 mM 0.32 mL 1.62 mL 3.24 mL
10 mM 0.16 mL 0.81 mL 1.62 mL
50 mM 0.03 mL 0.16 mL 0.32 mL

Molarity Calculator

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

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Product Datasheets

Safety Datasheet

References

References are publications that support the biological activity of the product.

Grimm et al (2016) Bright photoactivatable fluorophores for single-molecule imaging. Nat.Methods 13 985 PMID: 27776112

Li et al (2016) Real-time imaging of Huntingtin aggregates diverting target search and gene transcription. eLife 5 e17056 PMID: 27484239


If you know of a relevant reference for PA Janelia Fluor® 646, SE, please let us know.

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Citations for PA Janelia Fluor® 646, SE

Citations are publications that use Tocris products.

Currently there are no citations for PA Janelia Fluor® 646, SE. Do you know of a great paper that uses PA Janelia Fluor® 646, SE from Tocris? Please let us know.

Reviews for PA Janelia Fluor® 646, SE

Average Rating: 5 (Based on 1 Review.)

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PA-JF646 for tracking single recombinant proteins
By Anonymous on 02/26/2018
Assay Type: In Vitro
Species: Other

This product was used to label recombinant proteins and image them with single molecule sensitivity. The degree of labeling achieved was 1.4 (average). The photoactivation efficiency of the dye is excellent and can be used to track a few molecules at a time, for long periods. The dye is highly photostable and can be used to track proteins for ~10-15 seconds under ideal tracking conditions. I attach here an image before and after photoactivation of the dye. Upon photoactivation, single dye molecules conjugated to the protein are visible.

review image

Protocols for PA Janelia Fluor® 646, SE

The following protocol features additional information for the use of PA Janelia Fluor® 646, SE (Cat. No. 6150).

Literature in this Area

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