PA Janelia Fluor® 549, SE

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Cat.No. 6149 - PA Janelia Fluor 549, SE | C32H25N5O6 | CAS No. 1811539-42-0
Description: Yellow photoactivatable dye (Reactive group: NHS ester). Applications: super resolution microscopy (PALM), single molecule tracking and live cell imaging
Chemical Name: 2,5-Dioxo-1-pyrrolidinyl 3',6'-di-1-azetidinyl-2-diazo-2,3-dihydro-3-oxospiro[1H-indene-1,9'-[9H]xanthene]-6-carboxylate
Protocols (1)
Literature (1)

Biological Activity

Photoactivatable fluorescent dye; supplied as an NHS ester for coupling to primary amine groups. Non-fluorescent until activated at 365 nm. Photochemical quantum yield of uncaging = 2.2%, with improved yield upon protein conjugation. NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag® and SNAP-tag®. Suitable for single molecule tracking and super resolution microscopy in live cells, specifically live cell sptPALM and fixed cell PALM. Can be multiplexed with PA Janelia Fluor® 646, SE (Cat. No. 6150) to perform two-color sptPALM in live cells with activation at 405 nm. Cell permeable. Excitation maximum = 551 - 553 nm; emission maximum = 570 - 573 nm.

HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.

Licensing Information

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectral Viewer

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Technical Data

M. Wt 575.58
Formula C32H25N5O6
Storage Store at -20°C
CAS Number 1811539-42-0
PubChem ID 126569714
Smiles O=C(ON1C(CCC1=O)=O)C2=CC=C(C(C3=[N+]=[N-])=O)C(C43C5=CC=C(N6CCC6)C=C5OC7=CC(N8CCC8)=CC=C47)=C2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 5.76 10

Preparing Stock Solutions

The following data is based on the product molecular weight 575.58. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.1 mM 17.37 mL 86.87 mL 173.74 mL
0.5 mM 3.47 mL 17.37 mL 34.75 mL
1 mM 1.74 mL 8.69 mL 17.37 mL
5 mM 0.35 mL 1.74 mL 3.47 mL

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Product Datasheets

Certificate of Analysis / Product Datasheet
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References are publications that support the biological activity of the product.

Grimm et al (2016) Bright photoactivatable fluorophores for single-molecule imaging. Nat.Methods 13 985 PMID: 27776112

Li et al (2016) Real-time imaging of Huntingtin aggregates diverting target search and gene transcription. eLife 5 e17056 PMID: 27484239

Hong et al (2009) Phosphorylation of the RNA polymerase II C-terminal domain by TFIIH kinase is not essential for transcription of Saccharomyces cerevisiae genome. Proc.Natl.Acad.Sci.USA. 106 14276 PMID: 19666497

If you know of a relevant reference for PA Janelia Fluor® 549, SE, please let us know.

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Citations for PA Janelia Fluor® 549, SE

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Protocols for PA Janelia Fluor® 549, SE

The following protocol features additional information for the use of PA Janelia Fluor® 549, SE (Cat. No. 6149).

Literature in this Area

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