PA Janelia Fluor® 549, SE
Photoactivatable fluorescent dye; supplied as an NHS ester for coupling to primary amine groups. Non-fluorescent until activated at 365 nm. Photochemical quantum yield of uncaging = 2.2%, with improved yield upon protein conjugation. Compatible with self-labeling tag systems (e.g. HaloTag® and SNAPtag®). Suitable for single molecule tracking and super resolution microscopy in live cells, specifically live cell sptPALM and fixed cell PALM. Can be multiplexed with PA Janelia Fluor® 646, SE (Cat. No. 6150) to perform two-color sptPALM in live cells with activation at 405 nm. Cell permeable. Excitation maximum = 551 - 553 nm; emission maximum = 570 - 573 nm.
HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions
The following data is based on the product molecular weight 575.58. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||1.74 mL||8.69 mL||17.37 mL|
|5 mM||0.35 mL||1.74 mL||3.47 mL|
|10 mM||0.17 mL||0.87 mL||1.74 mL|
|50 mM||0.03 mL||0.17 mL||0.35 mL|
References are publications that support the products' biological activity.
Grimm et al (2016) Bright photoactivatable fluorophores for single-molecule imaging. Nat.Methods 13 985 PMID: 27776112
Li et al (2016) Real-time imaging of Huntingtin aggregates diverting target search and gene transcription. eLife 5 e17056 PMID: 27484239
If you know of a relevant reference for PA Janelia Fluor® 549, SE, please let us know.
View Related Products by Target
Keywords: PA Janelia Fluor 549, SE, supplier, PA, JF549, Photoactivatable, caged, Janelia, Fluor®, 549-SE, fluorescent, dyes, NHS, ester, self-labeling, tag, systems, microscopy, cell, permeable, super, resolution, Janelia, Fluor, Dyes, PA, Janelia, Fluor, Dyes, Janelia, Fluor, Dyes, Tocris Bioscience
Citations for PA Janelia Fluor® 549, SE
Citations are publications that use Tocris products.
Currently there are no citations for PA Janelia Fluor® 549, SE. Do you know of a great paper that uses PA Janelia Fluor® 549, SE from Tocris? If so please let us know.
Reviews for PA Janelia Fluor® 549, SE
There are currently no reviews for this product. Be the first to review PA Janelia Fluor® 549, SE and earn rewards!
Have you used PA Janelia Fluor® 549, SE?
Submit a review and receive an Amazon gift card.
$10US/$10CAN/€7/£6 gift card for a review without an image
$25US/$25CAN/€18/£15 gift card for a review with an image
*Offer only valid in the USA / Canada, UK and EuropeSubmit a Review
Protocols for PA Janelia Fluor® 549, SE
The following protocol features additional information for the use of PA Janelia Fluor® 549, SE (Cat. No. 6149).
Literature in this Area
Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* or download your copy today!
*Please note that Tocris will only send literature to established scientific business / institute addresses.
New Product Guide (Spring/Summer 2018)New
Our new product guide highlights over 200 new products added to the Tocris Bioscience range during the second half of 2017.
- 7-TM Receptors
- Enzyme-Linked Receptors
- Ion Channels
- Nuclear Receptors
- Cell Metabolism
- Fluorescent Imaging
- Signal Transduction
- Stem Cells