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Submit ReviewPA Janelia Fluor® 549, NHS ester
Description: Yellow photoactivatable dye (Reactive group: NHS ester). Application: super resolution microscopy (SRM), single molecule tracking and live cell imaging
Chemical Name: 2,5-Dioxo-1-pyrrolidinyl 3',6'-di-1-azetidinyl-2-diazo-2,3-dihydro-3-oxospiro[1H-indene-1,9'-[9H]xanthene]-6-carboxylate
Purity: ≥90% (HPLC)
Biological Activity for PA Janelia Fluor® 549, NHS ester
Key information: PA Janelia Fluor® 549, NHS ester is a yellow photoactivatable fluorescent dye; supplied with an NHS ester reactive group for the labeling of primary amines. Suitable for live and fixed cell imaging.
Application: Suitable for single molecule tracking and super resolution microscopy (SRM) in live cells, specifically sptPALM (live cells) and PALM (fixed cells) techniques.
Properties and Photophysical Data: Non-fluorescent until activated at 405 nm. NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag® and SNAP-tag®. Can be multiplexed with PA Janelia Fluor® 646, NHS ester (Cat. No. 6150) to perform two-color sptPALM in live cells with activation at 405 nm. Photochemical quantum yield of uncaging = 2.2%, with improved yield upon protein conjugation. Excitation and emission maxima (λ) are 551 - 553 nm and 570 - 573 nm, respectively.
Please see the protocol for further information on protein/antibody labeling and conjugation.
HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.
Janelia Fluor is a registered trademark of Howard Hughes Medical Institute.
This product has been recently renamed. The previous name for this product was PA Janelia Fluor® 549, SE.
Licensing Information
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus
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Technical Data for PA Janelia Fluor® 549, NHS ester
| M. Wt | 575.58 |
| Formula | C32H25N5O6 |
| Storage | Store at -20°C |
| Purity | ≥90% (HPLC) |
| CAS Number | 1811539-42-0 |
| PubChem ID | 126569714 |
| InChI Key | ZJZWRNGNJDEPEQ-UHFFFAOYSA-N |
| Smiles | O=C(ON1C(CCC1=O)=O)C2=CC=C(C(C3=[N+]=[N-])=O)C(C43C5=CC=C(N6CCC6)C=C5OC7=CC(N8CCC8)=CC=C47)=C2 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for PA Janelia Fluor® 549, NHS ester
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 5.76 | 10 |
Preparing Stock Solutions for PA Janelia Fluor® 549, NHS ester
The following data is based on the product molecular weight 575.58. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 17.37 mL | 86.87 mL | 173.74 mL |
| 0.5 mM | 3.47 mL | 17.37 mL | 34.75 mL |
| 1 mM | 1.74 mL | 8.69 mL | 17.37 mL |
| 5 mM | 0.35 mL | 1.74 mL | 3.47 mL |
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Product Datasheets for PA Janelia Fluor® 549, NHS ester
References for PA Janelia Fluor® 549, NHS ester
References are publications that support the biological activity of the product.
Grimm et al (2016) Bright photoactivatable fluorophores for single-molecule imaging. Nat.Methods 13 985 PMID: 27776112
Li et al (2016) Real-time imaging of Huntingtin aggregates diverting target search and gene transcription. eLife 5 e17056 PMID: 27484239
Hong et al (2009) Phosphorylation of the RNA polymerase II C-terminal domain by TFIIH kinase is not essential for transcription of Saccharomyces cerevisiae genome. Proc.Natl.Acad.Sci.USA. 106 14276 PMID: 19666497
If you know of a relevant reference for PA Janelia Fluor® 549, NHS ester, please let us know.
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1 Citation for PA Janelia Fluor® 549, NHS ester
Citations are publications that use Tocris products. Selected citations for PA Janelia Fluor® 549, NHS ester include:
Genshaft et al (2021) Live cell tagging tracking and isolation for spatial transcriptomics using photoactivatable cell dyes Nat Commun 12 4995 PMID: 34404785
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Protocols for PA Janelia Fluor® 549, NHS ester
The following protocol features additional information for the use of PA Janelia Fluor® 549, NHS ester (Cat. No. 6149).
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Fluorescent Dyes and Probes Research Product GuideUpdated
This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:
- Fluorescent Dyes, including Janelia Fluor® Dyes
- Fluorescent Probes for Advanced Microscopy and Live Cell Imaging
- Fluorescent Probes to Support In Vivo, Deep Tissue, and Bioluminescence Imaging
- Fluorescent Probes and Reagents for Organoids and 3D Cell Culture Imaging
- Aptamer-based RNA Imaging Reagents
- Fluorescent Probes for Imaging Bacteria
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- Tissue Clearing Kits and Reagents