Nucleic Acid Dye Green I

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Cat.No. 7246 - Nucleic Acid Dye Green I | C32H37ClN4S | CAS No. 2225748-05-8
Description: High affinity double-stranded DNA (dsDNA) probe; used in RT-LAMP and qPCR
Chemical Name: 2-((2-((3-(Dimethylamino)propyl)(propyl)amino)-1-phenylquinolin-4(1H)-ylidene)methyl)-3-methylbenzo[d]thiazol-3-ium chloride
Purity: ≥95% (HPLC)
Datasheet
Citations
Reviews
Literature (1)

Biological Activity

Nucleic Acid Dye Green I is an asymmetric cyanine dye that specifically binds to dsDNA. Frequently used dye in qPCR and is further used as a stain for in-gel visualization of DNA. Also used in the detection of amplification products in Loop-Mediated Isothermal Amplification (LAMP) assays; this method has been used for the detection of SARS-CoV-2 virus. When bound to dsDNA the fluorescence intensity is 1000-fold greater than when in the unbound form. Does not require washing or de-staining steps. Compatible with all commonly used fluorescence detection systems. Excitation maximum = 495 nm; emission maximum = 520 nm.

This product is sold as a DMSO stock.

Usage Information

  • Supplied as a 10,000x concentrate in DMSO.
  • Soluble in TE (10 mM Tris-HCl, 1 mM EDTA, pH 8.0), TBE, or TAE buffer.
  • Verify that the pH of the staining solution, at the temperature used for staining, is between 7.5 and 8.0 (preferably pH 8.0).
  • Use diluted samples within 24 hours to ensure maximal sensitivity.

Optical Data for Nucleic Acid Dye Green I

Nucleic Acid Dye Green I Dye Spectra
Emission Color Green
λabs 495 nm
λem 520 nm
Application Real Time PCR, Nucleic Acid Staining, RT-LAMP
Alternative For SYBR Green I

Technical Data

M. Wt 545.19
Formula C32H37ClN4S
Storage Store at -20°C
Purity ≥95% (HPLC)
CAS Number 2225748-05-8
PubChem ID 131742173
InChI Key IHHRNZWXTFDDLM-UHFFFAOYSA-M
Smiles CCCN(C(N(C1=C/2C=CC=C1)C3=CC=CC=C3)=CC2=C\C4=[N+](C)C5=CC=CC=C5S4)CCCN(C)C.[Cl-]

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solubility Soluble in DMSO (supplied as a 10,000x concentrate in DMSO)

References

References are publications that support the biological activity of the product.

Karlsen et al (1995) SYBR Green I DNA staining increases the detection sensitivity of viruses by polymerase chain reaction. J.Virol.Methods 55 153 PMID: 8576305

Bookout et al (2006) High-throughput real-time quantitative reverse transcription PCR. Curr.Protoc.Mol.Biol. doi: 10.1002/0471142 PMID: 18265376

Lamb et al (2018) Rapid detection of Zika virus in urine samples and infected mosquitos by Reverse Transcription-Loop-Mediated Isothermal Amplification. Sci.Rep. 8 3803 PMID: 29491389

Lamb et al (2020) Rapid detection of novel coronavirus/Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) by reverse transcription-loop-mediated isothermal amplification PLoS One 15 e0234682 PMID: 32530929


If you know of a relevant reference for Nucleic Acid Dye Green I, please let us know.

Keywords: Nucleic Acid Dye Green I, Nucleic Acid Dye Green I supplier, High, affinity, double-stranded, DNA, (dsDNA), probe, RT-LAMP, Reverse, Transcription, Loop-Mediated, Isothermal, Amplification, SARS-CoV-2, Zika, RTPCR, RT-PCR, qPCR, q_PCR, Fluorescent, Stains, RNA, Probes, 7246, Tocris Bioscience

Citations for Nucleic Acid Dye Green I

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