Nucleic Acid Dye Green I

Discontinued Product

Nucleic Acid Dye Green I (Cat. No. 7246) has been withdrawn from sale for commercial reasons.
Description: High affinity double-stranded DNA (dsDNA) probe; used in RT-LAMP and qPCR
Chemical Name: 2-((2-((3-(Dimethylamino)propyl)(propyl)amino)-1-phenylquinolin-4(1H)-ylidene)methyl)-3-methylbenzo[d]thiazol-3-ium chloride
Purity: ≥95% (HPLC)
Literature (1)

Biological Activity for Nucleic Acid Dye Green I

Nucleic Acid Dye Green I is an asymmetric cyanine dye that specifically binds to dsDNA. Frequently used dye in qPCR and is further used as a stain for in-gel visualization of DNA. Also used in the detection of amplification products in Loop-Mediated Isothermal Amplification (LAMP) assays; this method has been used for the detection of SARS-CoV-2 virus. When bound to dsDNA the fluorescence intensity is 1000-fold greater than when in the unbound form. Does not require washing or de-staining steps. Compatible with all commonly used fluorescence detection systems. Excitation maximum = 495 nm; emission maximum = 520 nm.

This product is sold as a DMSO stock.

Usage Information for Nucleic Acid Dye Green I

  • Supplied as a 10,000x concentrate in DMSO.
  • Soluble in TE (10 mM Tris-HCl, 1 mM EDTA, pH 8.0), TBE, or TAE buffer.
  • Verify that the pH of the staining solution, at the temperature used for staining, is between 7.5 and 8.0 (preferably pH 8.0).
  • Use diluted samples within 24 hours to ensure maximal sensitivity.

Optical Data for Nucleic Acid Dye Green I

Nucleic Acid Dye Green I Dye Spectra
Emission Color Green
λabs 495 nm
λem 520 nm
Application Real Time PCR, Nucleic Acid Staining, RT-LAMP
Alternative For SYBR® Green I

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Technical Data for Nucleic Acid Dye Green I

M. Wt 545.19
Formula C32H37ClN4S
Storage Store at -20°C
Purity ≥95% (HPLC)
CAS Number 2225748-05-8
PubChem ID 131742173
Smiles CCCN(C(N(C1=C/2C=CC=C1)C3=CC=CC=C3)=CC2=C\C4=[N+](C)C5=CC=CC=C5S4)CCCN(C)C.[Cl-]

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Product Datasheets for Nucleic Acid Dye Green I

References for Nucleic Acid Dye Green I

References are publications that support the biological activity of the product.

Karlsen et al (1995) SYBR Green I DNA staining increases the detection sensitivity of viruses by polymerase chain reaction. J.Virol.Methods 55 153 PMID: 8576305

Bookout et al (2006) High-throughput real-time quantitative reverse transcription PCR. Curr.Protoc.Mol.Biol. doi: 10.1002/0471142 PMID: 18265376

Lamb et al (2018) Rapid detection of Zika virus in urine samples and infected mosquitos by Reverse Transcription-Loop-Mediated Isothermal Amplification. Sci.Rep. 8 3803 PMID: 29491389

Lamb et al (2020) Rapid detection of novel coronavirus/Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) by reverse transcription-loop-mediated isothermal amplification PLoS One 15 e0234682 PMID: 32530929

Keywords: Nucleic Acid Dye Green I, Nucleic Acid Dye Green I supplier, High, affinity, double-stranded, DNA, (dsDNA), probe, RT-LAMP, Reverse, Transcription, Loop-Mediated, Isothermal, Amplification, SARS-CoV-2, Zika, RTPCR, RT-PCR, qPCR, q_PCR, Fluorescent, Stains, RNA, Probes, Loop-mediated, and, RT-qPCR, 7246, Tocris Bioscience

Citations for Nucleic Acid Dye Green I

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Currently there are no citations for Nucleic Acid Dye Green I.

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