NPPB

Pricing Availability   Qty
Description: Inhibits calcium-sensitive chloride currents
Chemical Name: 5-Nitro-2-(3-phenylpropylamino)benzoic acid
Purity: ≥99% (HPLC)
Datasheet
Citations (10)
Reviews (1)
Literature (4)
Pathways (1)

Biological Activity for NPPB

Inhibits calcium-sensitive chloride currents (10 μM). Putative GPR35 agonist.

Compound Libraries for NPPB

NPPB is also offered as part of the Tocriscreen 2.0 Max. Find out more about compound libraries available from Tocris.

Technical Data for NPPB

M. Wt 300.31
Formula C16H16N2O4
Storage Store at RT
Purity ≥99% (HPLC)
CAS Number 107254-86-4
PubChem ID 4549
InChI Key WBSMIPAMAXNXFS-UHFFFAOYSA-N
Smiles OC(=O)C1=CC(=CC=C1NCCCC1=CC=CC=C1)[N+]([O-])=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for NPPB

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 30.03 100
ethanol 6.01 20

Preparing Stock Solutions for NPPB

The following data is based on the product molecular weight 300.31. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.33 mL 16.65 mL 33.3 mL
5 mM 0.67 mL 3.33 mL 6.66 mL
10 mM 0.33 mL 1.66 mL 3.33 mL
50 mM 0.07 mL 0.33 mL 0.67 mL

Molarity Calculator

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Reconstitution Calculator

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Product Datasheets for NPPB

Certificate of Analysis / Product Datasheet
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References for NPPB

References are publications that support the biological activity of the product.

Keeling et al (1991) Effects of NPPB (5-nitro-1-(3-phenylpropylamino)benzoic acid on chloride transport in intestinal tissues and the T84 cell line. Biochim.Biophys.Acta 115 42 PMID: 1720331

Kirkup et al (1996) Investigation of the effects of 5-nitro-2-(3-phenylpropylamino)-benzoic acid (NPPB) on membrane currents in rat portal vein. Br.J.Pharmacol. 117 175 PMID: 8825360

Taniguchi et al (2008) 5-nitro-2-(3-phenylpropylamino)benzoic acid is a GPR35 agonist Pharmacology 82 245 PMID: 18818509

Wangemann et al (1986) Cl--channel blockers in the thick ascending limb of the loop of Henle. Structure-activity relationship. Pflugers Arch. 407 S128 PMID: 2434915


If you know of a relevant reference for NPPB, please let us know.

View Related Products by Product Action

View all Calcium-activated Chloride Channel Blockers

Keywords: NPPB, NPPB supplier, Chloride, channel, blockers, Cl-, Channels, GPR35, Ca2+-Activated, 0593, Tocris Bioscience

10 Citations for NPPB

Citations are publications that use Tocris products. Selected citations for NPPB include:

Silverman et al (2008) Probenecid, a gout remedy, inhibits pannexin 1 channels. Am J Physiol Cell Physiol 295 C761 PMID: 18596212

Grant et al (2013) Ionic currents of human trabecular meshwork cells from control and glaucoma subjects. Biol Proced Online 246 167 PMID: 23135060

Fioretti et al (2005) Intermediate-conductance Ca2+-activated K+ channel is expressed in C2C12 myoblasts and is downregulated during myogenesis. Am J Physiol Cell Physiol 289 C89 PMID: 15743891

Li et al (2005) Hemolysis of erythrocytes by granulysin-derived peptides but not by granulysin. Antimicrob Agents Chemother 49 388 PMID: 15616319


Do you know of a great paper that uses NPPB from Tocris? Please let us know.

Reviews for NPPB

Average Rating: 5 (Based on 1 Review.)

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A potent chloride channel blocker.
By Anonymous on 02/19/2018
Assay Type: Ex Vivo
Species: Rat

It blocks calcium-activated chloride currents. We used NPPB at 50-100 microM to block chloride channels. It dissolves well in DMSO


Literature in this Area

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