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Description: Inhibits calcium-sensitive chloride currents
Chemical Name: 5-Nitro-2-(3-phenylpropylamino)benzoic acid
Purity: ≥99% (HPLC)
Biological Activity for NPPB
Inhibits calcium-sensitive chloride currents (10 μM). Putative GPR35 agonist.
Compound Libraries for NPPB
NPPB is also offered as part of the Tocriscreen 2.0 Max. Find out more about compound libraries available from Tocris.
Technical Data for NPPB
| M. Wt | 300.31 |
| Formula | C16H16N2O4 |
| Storage | Store at RT |
| Purity | ≥99% (HPLC) |
| CAS Number | 107254-86-4 |
| PubChem ID | 4549 |
| InChI Key | WBSMIPAMAXNXFS-UHFFFAOYSA-N |
| Smiles | OC(=O)C1=CC(=CC=C1NCCCC1=CC=CC=C1)[N+]([O-])=O |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for NPPB
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 30.03 | 100 | |
| ethanol | 6.01 | 20 |
Preparing Stock Solutions for NPPB
The following data is based on the product molecular weight 300.31. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 3.33 mL | 16.65 mL | 33.3 mL |
| 5 mM | 0.67 mL | 3.33 mL | 6.66 mL |
| 10 mM | 0.33 mL | 1.66 mL | 3.33 mL |
| 50 mM | 0.07 mL | 0.33 mL | 0.67 mL |
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Product Datasheets for NPPB
References for NPPB
References are publications that support the biological activity of the product.
Keeling et al (1991) Effects of NPPB (5-nitro-1-(3-phenylpropylamino)benzoic acid on chloride transport in intestinal tissues and the T84 cell line. Biochim.Biophys.Acta 115 42 PMID: 1720331
Kirkup et al (1996) Investigation of the effects of 5-nitro-2-(3-phenylpropylamino)-benzoic acid (NPPB) on membrane currents in rat portal vein. Br.J.Pharmacol. 117 175 PMID: 8825360
Taniguchi et al (2008) 5-nitro-2-(3-phenylpropylamino)benzoic acid is a GPR35 agonist Pharmacology 82 245 PMID: 18818509
Wangemann et al (1986) Cl--channel blockers in the thick ascending limb of the loop of Henle. Structure-activity relationship. Pflugers Arch. 407 S128 PMID: 2434915
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Keywords: NPPB, NPPB supplier, Chloride, channel, blockers, Cl-, Channels, GPR35, Ca2+-Activated, 0593, Tocris Bioscience
10 Citations for NPPB
Citations are publications that use Tocris products. Selected citations for NPPB include:
Sagheddu et al (2010) Calcium concentration jumps reveal dynamic ion selectivity of calcium-activated chloride currents in mouse olfactory sensory neurons and TMEM16b-transfected HEK 293T cells. J Physiol 588 4189 PMID: 20837642
Fei et al (2009) Stimulation of mucosal secretion by lubipro. (SPI-0211) in guinea pig small intestine and colon. Am J Physiol Gastrointest Liver Physiol 296 G823 PMID: 19179625
Rastogi et al (2021) Mycobacterium tuberculosis inhibits the NLRP3 inflammasome activation via its phosphokinase PknF PLoS Pathog 17 e1009712 PMID: 34324582
Harper and Poole (2013) Chloride channels are necessary for full platelet phosphatidylserine exposure and procoagulant activity. J Membr Biol 4 e969 PMID: 24357800
Grant et al (2013) Ionic currents of human trabecular meshwork cells from control and glaucoma subjects. Biol Proced Online 246 167 PMID: 23135060
Silverman et al (2008) Probenecid, a gout remedy, inhibits pannexin 1 channels. Am J Physiol Cell Physiol 295 C761 PMID: 18596212
Morethson (2015) Extracellular fluid flow and chloride content modulate H(+) transport by osteoclasts. Cell Death Dis 16 20 PMID: 26271334
Sun et al (2011) Lubiprostone reverses the inhibitory action of mor. on mucosal secretion in human small intestine. Dig Dis Sci 56 330 PMID: 21181441
Fioretti et al (2005) Intermediate-conductance Ca2+-activated K+ channel is expressed in C2C12 myoblasts and is downregulated during myogenesis. Am J Physiol Cell Physiol 289 C89 PMID: 15743891
Li et al (2005) Hemolysis of erythrocytes by granulysin-derived peptides but not by granulysin. Antimicrob Agents Chemother 49 388 PMID: 15616319
Do you know of a great paper that uses NPPB from Tocris? Please let us know.
Reviews for NPPB
Average Rating: 5 (Based on 1 Review.)
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A potent chloride channel blocker.
By
Anonymous on 02/19/2018
Assay Type: Ex Vivo
Species: Rat
It blocks calcium-activated chloride currents. We used NPPB at 50-100 microM to block chloride channels. It dissolves well in DMSO
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