NPPB

Pricing Availability Delivery Time Qty
Cat.No. 0593 - NPPB | C16H16N2O4 | CAS No. 107254-86-4
Description: Inhibits calcium-sensitive chloride currents
Chemical Name: 5-Nitro-2-(3-phenylpropylamino)benzoic acid
Purity: ≥99% (HPLC)
Datasheet
Citations (5)
Literature
Pathways

Biological Activity

Inhibits calcium-sensitive chloride currents (10 μM). Putative GPR35 agonist.

Compound Libraries

NPPB is also offered as part of the Tocriscreen Plus. Find out more about compound libraries available from Tocris.

Technical Data

M. Wt 300.31
Formula C16H16N2O4
Storage Store at RT
Purity ≥99% (HPLC)
CAS Number 107254-86-4
PubChem ID 4549
InChI Key WBSMIPAMAXNXFS-UHFFFAOYSA-N
Smiles OC(=O)C1=CC(=CC=C1NCCCC1=CC=CC=C1)[N+]([O-])=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 30.03 100
ethanol 6.01 20

Preparing Stock Solutions

The following data is based on the product molecular weight 300.31. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.33 mL 16.65 mL 33.3 mL
5 mM 0.67 mL 3.33 mL 6.66 mL
10 mM 0.33 mL 1.66 mL 3.33 mL
50 mM 0.07 mL 0.33 mL 0.67 mL

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Product Datasheets

Certificate of Analysis / Product Datasheet
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Safety Datasheet

References

References are publications that support the products' biological activity.

Keeling et al (1991) Effects of NPPB (5-nitro-1-(3-phenylpropylamino)benzoic acid on chloride transport in intestinal tissues and the T84 cell line. Biochim.Biophys.Acta 115 42 PMID: 1720331

Kirkup et al (1996) Investigation of the effects of 5-nitro-2-(3-phenylpropylamino)-benzoic acid (NPPB) on membrane currents in rat portal vein. Br.J.Pharmacol. 117 175 PMID: 8825360

Taniguchi et al (2008) 5-nitro-2-(3-phenylpropylamino)benzoic acid is a GPR35 agonist Pharmacology 82 245 PMID: 18818509

Wangemann et al (1986) Cl--channel blockers in the thick ascending limb of the loop of Henle. Structure-activity relationship. Pflugers Arch. 407 S128 PMID: 2434915


If you know of a relevant reference for NPPB, please let us know.

View Related Products by Product Action

View all Calcium-activated Chloride Channel Blockers

Keywords: Chloride channel blockers Cl- Channels Ca2+-Activated Chloride Channels

5 Citations for NPPB

Citations are publications that use Tocris products. Selected citations for NPPB include:

Morethson (2015) Extracellular fluid flow and chloride content modulate H(+) transport by osteoclasts. Cell Death Dis 16 20 PMID: 26271334

Grant et al (2013) Ionic currents of human trabecular meshwork cells from control and glaucoma subjects. Biol Proced Online 246 167 PMID: 23135060

Harper and Poole (2013) Chloride channels are necessary for full platelet phosphatidylserine exposure and procoagulant activity. J Membr Biol 4 e969 PMID: 24357800

Silverman et al (2008) Probenecid, a gout remedy, inhibits pannexin 1 channels. Am J Physiol Cell Physiol 295 C761 PMID: 18596212

Li et al (2005) Hemolysis of erythrocytes by granulysin-derived peptides but not by granulysin. Antimicrob Agents Chemother 49 388 PMID: 15616319


Do you know of a great paper that uses NPPB from Tocris? If so please let us know.

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Literature in this Area

Cardiovascular

Cardiovascular Research Product Guide

A collection of over 250 products for cardiovascular research, the guide includes research tools for the study of:

  • Hypertension
  • Thrombosis and Hemostasis
  • Atherosclerosis
  • Myocardial Infarction
  • Ischemia/Reperfusion Injury
  • Arrhythmias
  • Heart Failure
Programmed Cell Death

Programmed Cell Death Poster

There are two currently recognized forms of programmed cell death: apoptosis and necroptosis. This poster summarizes the signaling pathways involved in apoptosis, necroptosis and cell survival following death receptor activation, and highlights the influence of the molecular switch, cFLIP, on cell fate.

Pathways for NPPB

Apoptosis

Apoptosis Signaling Pathway

Apoptosis is a physiological process for cell death that is critical during aging and development. It may also be referred to as cell 'suicide'. Apoptosis can be triggered by events both inside and outside of the cell.

Protocols

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