Inhibits calcium-sensitive chloride currents (10 μM). Putative GPR35 agonist.
|Storage||Store at RT|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions
The following data is based on the product molecular weight 300.31. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||3.33 mL||16.65 mL||33.3 mL|
|5 mM||0.67 mL||3.33 mL||6.66 mL|
|10 mM||0.33 mL||1.66 mL||3.33 mL|
|50 mM||0.07 mL||0.33 mL||0.67 mL|
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.
References are publications that support the products' biological activity.
Keeling et al (1991) Effects of NPPB (5-nitro-1-(3-phenylpropylamino)benzoic acid on chloride transport in intestinal tissues and the T84 cell line. Biochim.Biophys.Acta 115 42 PMID: 1720331
Kirkup et al (1996) Investigation of the effects of 5-nitro-2-(3-phenylpropylamino)-benzoic acid (NPPB) on membrane currents in rat portal vein. Br.J.Pharmacol. 117 175 PMID: 8825360
Taniguchi et al (2008) 5-nitro-2-(3-phenylpropylamino)benzoic acid is a GPR35 agonist Pharmacology 82 245 PMID: 18818509
Wangemann et al (1986) Cl--channel blockers in the thick ascending limb of the loop of Henle. Structure-activity relationship. Pflugers Arch. 407 S128 PMID: 2434915
If you know of a relevant reference for NPPB, please let us know.
View Related Products by Product Action
Keywords: Chloride channel blockers Cl- Channels Ca2+-Activated Chloride Channels
5 Citations for NPPB
Citations are publications that use Tocris products. Selected citations for NPPB include:
Morethson (2015) Extracellular fluid flow and chloride content modulate H(+) transport by osteoclasts. Cell Death Dis 16 20 PMID: 26271334
Grant et al (2013) Ionic currents of human trabecular meshwork cells from control and glaucoma subjects. Biol Proced Online 246 167 PMID: 23135060
Harper and Poole (2013) Chloride channels are necessary for full platelet phosphatidylserine exposure and procoagulant activity. J Membr Biol 4 e969 PMID: 24357800
Silverman et al (2008) Probenecid, a gout remedy, inhibits pannexin 1 channels. Am J Physiol Cell Physiol 295 C761 PMID: 18596212
Li et al (2005) Hemolysis of erythrocytes by granulysin-derived peptides but not by granulysin. Antimicrob Agents Chemother 49 388 PMID: 15616319
Do you know of a great paper that uses NPPB from Tocris? If so please let us know.
Literature in this Area
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