Caged fluorescent pH indicator. Rapidly releases the fluorophore HPTS (pyranine) (pKa 7.25) upon two-photon excitation (>3000 s-1). Routinely used for photolysis calibration. Suitable for use in small-compartment diffusion studies.
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions
The following data is based on the product molecular weight 673.53. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||1.48 mL||7.42 mL||14.85 mL|
|5 mM||0.3 mL||1.48 mL||2.97 mL|
|10 mM||0.15 mL||0.74 mL||1.48 mL|
|50 mM||0.03 mL||0.15 mL||0.3 mL|
References are publications that support the biological activity of the product.
Willoughby et al (1998) Comparisons of simultaneous pH measurements made with 8-hydroxypyrene-1,3,6-trisulphonic acid (HPTS) and pH-sensitive microelectrodes in snail neurones. Pflugers Arch. 436 615 PMID: 9683736
Jasuja et al (1999) Chemotactic responses of Escherichia coli to small jumps of photoreleased L-aspartate. Biophys.J. 76 1706 PMID: 10049350
Kiskin et al (2002) The efficiency of two-photon photolysis of a "caged " fluorophore, o-1-(2-nitrophenyl)ethylpyranine, in relation to photodamage of synaptic terminals. Eur.Biophys.J. 30 588 PMID: 11908850
Bloodgood and Sabatini (2005) Neuronal activity regulates diffusion across the neck of dendritic spines. Science 310 866 PMID: 16272125
Trigo et al (2009) Laser photolysis of caged compounds at 405 nm: photochemical advantages, localisation, phototoxicity and methods for calibration. J.Neurosci.Methods 180 9 PMID: 19427524
Canepari et al (2001) Photochemical and pharmacological evaluation of 7-nitroindolinyl-and 4-methoxy-7-nitroindolinyl-amino acids as novel, fast caged neurotransmitters. J.Neurosci.Methods. 112 29 PMID: 11640955
Soler-Llavina et al (2006) Synapse-specific plasticity and compartmentalized signaling in cerebellar stellate cells. Nat.Neurosci. 9 798 PMID: 16680164
If you know of a relevant reference for NPE-caged-HPTS, please let us know.
View Related Products by Target
Keywords: NPE-caged-HPTS, NPE-caged-HPTS supplier, Caged, fluorescent, pH, indicator, Fluorescent, Probes, Compounds, Ion, Indicators, Other, 2919, Tocris Bioscience
Citations for NPE-caged-HPTS
Citations are publications that use Tocris products.
Currently there are no citations for NPE-caged-HPTS. Do you know of a great paper that uses NPE-caged-HPTS from Tocris? Please let us know.
Reviews for NPE-caged-HPTS
There are currently no reviews for this product. Be the first to review NPE-caged-HPTS and earn rewards!
Have you used NPE-caged-HPTS?
Submit a review and receive an Amazon gift card.
$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Literature in this Area
Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* or download your copy today!
*Please note that Tocris will only send literature to established scientific business / institute addresses.
New Product Guide (Spring/Summer 2018)
Our new product guide highlights over 200 new products added to the Tocris Bioscience range during the second half of 2017.
- 7-TM Receptors
- Enzyme-Linked Receptors
- Ion Channels
- Nuclear Receptors
- Cell Metabolism
- Fluorescent Imaging
- Signal Transduction
- Stem Cells