Caged fluorescent pH indicator. Rapidly releases the fluorophore HPTS (pyranine) (pKa 7.25) upon two-photon excitation (>3000 s-1). Routinely used for photolysis calibration. Suitable for use in small-compartment diffusion studies.
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions
The following data is based on the product molecular weight 673.53. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||1.48 mL||7.42 mL||14.85 mL|
|5 mM||0.3 mL||1.48 mL||2.97 mL|
|10 mM||0.15 mL||0.74 mL||1.48 mL|
|50 mM||0.03 mL||0.15 mL||0.3 mL|
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.
References are publications that support the products' biological activity.
Willoughby et al (1998) Comparisons of simultaneous pH measurements made with 8-hydroxypyrene-1,3,6-trisulphonic acid (HPTS) and pH-sensitive microelectrodes in snail neurones. Pflugers Arch. 436 615 PMID: 9683736
Jasuja et al (1999) Chemotactic responses of Escherichia coli to small jumps of photoreleased L-aspartate. Biophys.J. 76 1706 PMID: 10049350
Kiskin et al (2002) The efficiency of two-photon photolysis of a "caged " fluorophore, o-1-(2-nitrophenyl)ethylpyranine, in relation to photodamage of synaptic terminals. Eur.Biophys.J. 30 588 PMID: 11908850
Bloodgood and Sabatini (2005) Neuronal activity regulates diffusion across the neck of dendritic spines. Science 310 866 PMID: 16272125
Trigo et al (2009) Laser photolysis of caged compounds at 405 nm: photochemical advantages, localisation, phototoxicity and methods for calibration. J.Neurosci.Methods 180 9 PMID: 19427524
Canepari et al (2001) Photochemical and pharmacological evaluation of 7-nitroindolinyl-and 4-methoxy-7-nitroindolinyl-amino acids as novel, fast caged neurotransmitters. J.Neurosci.Methods. 112 29 PMID: 11640955
Soler-Llavina et al (2006) Synapse-specific plasticity and compartmentalized signaling in cerebellar stellate cells. Nat.Neurosci. 9 798 PMID: 16680164
If you know of a relevant reference for NPE-caged-HPTS, please let us know.
Keywords: Caged fluorescent pH indicator Fluorescent Probes Compounds Fluorescent Probes
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