NHS-LC-Biotin

Cat. No. 7301 Submit a Review Datasheet / COA / SDS

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Description: Amine reactive biotinylation reagent, cell permeable

Chemical Name: 6-[[5-[(3aS,4S,6aR)-Hexahydro-2-oxo-1H-thieno[3,4-d]imidazol-4-yl]-1-oxopentyl]amino]hexanoic acid 2,5-dioxo-1-pyrrolidinyl ester

Purity: ≥90% (HPLC)

Datasheet

Citations

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Biological Activity Technical Data Solubility Calculators Datasheets References

Biological Activity for NHS-LC-Biotin

NHS-LC-Biotin is a membrane permeable amine reactive biotinylation reagent used to attach biotin to primary amines with a 6-atom chain (22.4 angstroms) linker. NHS-LC-Biotin is commonly used to label proteins and antibodies. Reactive in pH 7-9.

This product should be dissolved in DMSO (solubility = 100 mM) or DMF prior to further dilution in aqueous buffers.

Technical Data for NHS-LC-Biotin

M. Wt	454.54
Formula	C₂₀H₃₀N₄O₆S
Storage	Store at -20°C
Purity	≥90% (HPLC)
CAS Number	72040-63-2
PubChem ID	83874
InChI Key	UVGHPGOONBRLCX-NJSLBKSFSA-N
Smiles	C(CCCC(NCCCCCC(ON1C(=O)CCC1=O)=O)=O)[C@H]2[C@@]3([C@](CS2)(NC(=O)N3)[H])[H]

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for NHS-LC-Biotin

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	45.45	100
	DMF	45.45	100

Preparing Stock Solutions for NHS-LC-Biotin

The following data is based on the product molecular weight 454.54. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.2 mL	11 mL	22 mL
5 mM	0.44 mL	2.2 mL	4.4 mL
10 mM	0.22 mL	1.1 mL	2.2 mL
50 mM	0.04 mL	0.22 mL	0.44 mL

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

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Product Datasheets for NHS-LC-Biotin

Certificate of Analysis / Product Datasheet

Safety Datasheet

References for NHS-LC-Biotin

References are publications that support the biological activity of the product.

Kamperman et al (2019) Spatiotemporal material functionalization via competitive supramolecular complexation of avidin and biotin analogs. Nat.Commun. 10 4347 PMID: 31554812

Wang et al (2020) An array of 60,000 antibodies for proteome-scale antibody generation and target discovery. Sci.Adv. 6 eaax2271 PMID: 32195335

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Biochemicals and Molecular Biology

Keywords: NHS-LC-Biotin, NHS-LC-Biotin supplier, NHSLCBiotin, membrane, permeable, biotinylation, reagents, primary, amine, labels, NHS, Biotin, Biochemicals, and, Molecular, Biology, 7301, Tocris Bioscience

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