Pricing Availability   Qty
Description: Amine reactive biotinylation reagent, cell permeable
Chemical Name: 6-[[5-[(3aS,4S,6aR)-Hexahydro-2-oxo-1H-thieno[3,4-d]imidazol-4-yl]-1-oxopentyl]amino]hexanoic acid 2,5-dioxo-1-pyrrolidinyl ester
Purity: ≥90% (HPLC)
Citations (1)

Biological Activity for NHS-LC-Biotin

NHS-LC-Biotin is a membrane permeable amine reactive biotinylation reagent used to attach biotin to primary amines with a 6-atom chain (22.4 angstroms) linker. NHS-LC-Biotin is commonly used to label proteins and antibodies. Reactive in pH 7-9.

This product should be dissolved in DMSO (solubility = 100 mM) or DMF prior to further dilution in aqueous buffers.

Technical Data for NHS-LC-Biotin

M. Wt 454.54
Formula C20H30N4O6S
Storage Store at -20°C
Purity ≥90% (HPLC)
CAS Number 72040-63-2
PubChem ID 83874
Smiles C(CCCC(NCCCCCC(ON1C(=O)CCC1=O)=O)=O)[C@H]2[C@@]3([C@](CS2)(NC(=O)N3)[H])[H]

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for NHS-LC-Biotin

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 45.45 100
DMF 45.45 100

Preparing Stock Solutions for NHS-LC-Biotin

The following data is based on the product molecular weight 454.54. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.2 mL 11 mL 22 mL
5 mM 0.44 mL 2.2 mL 4.4 mL
10 mM 0.22 mL 1.1 mL 2.2 mL
50 mM 0.04 mL 0.22 mL 0.44 mL

Molarity Calculator

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Reconstitution Calculator

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Dilution Calculator

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Product Datasheets for NHS-LC-Biotin

References for NHS-LC-Biotin

References are publications that support the biological activity of the product.

Kamperman et al (2019) Spatiotemporal material functionalization via competitive supramolecular complexation of avidin and biotin analogs. Nat.Commun. 10 4347 PMID: 31554812

Wang et al (2020) An array of 60,000 antibodies for proteome-scale antibody generation and target discovery. Sci.Adv. 6 eaax2271 PMID: 32195335

If you know of a relevant reference for NHS-LC-Biotin, please let us know.

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Keywords: NHS-LC-Biotin, NHS-LC-Biotin supplier, NHSLCBiotin, membrane, permeable, biotinylation, reagents, primary, amine, labels, NHS, Biotin, Biochemicals, and, Molecular, Biology, 7301, Tocris Bioscience

1 Citation for NHS-LC-Biotin

Citations are publications that use Tocris products. Selected citations for NHS-LC-Biotin include:

Michael F et al (2012) High throughput functional assays for P2X receptors. Curr Protoc Pharmacol Chapter 9 Unit 9.15. PMID: 22684723

Do you know of a great paper that uses NHS-LC-Biotin from Tocris? Please let us know.

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