NH 125

Pricing Availability   Qty
Description: CaM kinase III (eEF-2 kinase) inhibitor
Chemical Name: 1-Hexadecyl-2-methyl-3-(phenylmethyl)-1H-imidazolium iodide
Citations (5)

Biological Activity for NH 125

NH 125 is an eukaryotic elongation factor 2 (eEF-2) kinase (CaMK III) inhibitor (IC50 = 60 nM) that displays 125-fold, > 1300-fold and > 1500-fold selectivity over PKC, PKA and CaMK II respectively. Decreases the viability of a variety of cancer cell lines (IC50 values are 0.7 - 4.8 μM) and blocks G1/S cell cycle progression. Shown to induce eEF-2 phosphorylation in cancer cells. Also an effective antibacterial agent; inhibits histidine protein kinase in vitro (IC50 = 6.6 μM) and in vivo.

Technical Data for NH 125

M. Wt 524.56
Formula C27H45IN2
Storage Desiccate at -20°C
CAS Number 278603-08-0
PubChem ID 10436839

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for NH 125

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 52.46 100
ethanol 39.34 75

Preparing Stock Solutions for NH 125

The following data is based on the product molecular weight 524.56. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.91 mL 9.53 mL 19.06 mL
5 mM 0.38 mL 1.91 mL 3.81 mL
10 mM 0.19 mL 0.95 mL 1.91 mL
50 mM 0.04 mL 0.19 mL 0.38 mL

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References for NH 125

References are publications that support the biological activity of the product.

Yamamoto et al (2001) Antibacterial agents that inhibit histidine protein kinase YycG of Bacillus subtilis. Biosci.Biotechnol.Biochem. 65 2306 PMID: 11758928

Arora et al (2003) Identification and characterization of an inhibitor of eukaryotic elongation factor 2 kinase against human cancer cell lines. Cancer Res. 63 6894 PMID: 14583488

Arora et al (2004) P-glycoprotein mediates resistance to histidine kinase inhibitors. Mol.Pharmacol. 66 460 PMID: 15322237

Chen (2011) 1-Benzyl-3-cetyl-2-methylimidazolium iodide (NH125) induces phosphorylation of eukaryotic elongation factor-2 (eEF2): a cautionary note on the anticancer mechanism of an eEF2 kinase inhibitor. J.Biol.Chem. 286 43951 PMID: 22020937

If you know of a relevant reference for NH 125, please let us know.

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Keywords: NH 125, NH 125 supplier, CaM, kinase, III, CAMK, eEF-2, inhibitors, inhibits, Histidine, protein, kinases, Calmodulin-Activated, calmodulin-dependent, Protein, NH125, Kinase, Other, Kinases, 3439, Tocris Bioscience

5 Citations for NH 125

Citations are publications that use Tocris products. Selected citations for NH 125 include:

Yi et al (2013) Inhibition of elongation factor-2 kinase augments the antitumor activity of Temozolomide against glioma. PLoS One 8 e81345 PMID: 24303044

Kim et al (2015) Identification of an Antimicrobial Agent Effective against Methicillin-Resistant Staphylococcus aureus Persisters Using a Fluorescence-Based Screening Strategy. PLoS One 10 e0127640 PMID: 26039584

Xu et al (2017) Neurons secrete miR-132-containing exosomes to regulate brain vascular integrity. Cell Res 27 882 PMID: 28429770

Francis et al (2013) Mechanistic insight into inhibition of two-component system signaling. Medchemcomm 4 269 PMID: 23336064

Do you know of a great paper that uses NH 125 from Tocris? Please let us know.

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