ML 7 hydrochloride

Pricing Availability   Qty
Description: Selective inhibitor of myosin light chain kinase
Chemical Name: Hexahydro-1-[(5-iodo-1-naphthalenyl)sulfonyl]-1H-1,4-diazepine hydrochloride
Purity: ≥98% (HPLC)
Citations (11)
Reviews (2)

Biological Activity for ML 7 hydrochloride

ML 7 hydrochloride is a selective MLCK inhibitor (Ki = 0.3 μM). Exhibits more potent inhibition than ML 9 hydrochloride (Cat. No. 0431) Displays reversible, ATP-competitive inhibition of Ca2+-calmodulin-dependent and -independent smooth muscle MLCKs. Inhibits proplatelet formation and stabilization.

Technical Data for ML 7 hydrochloride

M. Wt 452.74
Formula C15H17IN2O2S.HCl
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 110448-33-4
PubChem ID 9803932
Smiles IC1=C(C=CC=C2S(N3CCCNCC3)(=O)=O)C2=CC=C1.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for ML 7 hydrochloride

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 22.64 50

Preparing Stock Solutions for ML 7 hydrochloride

The following data is based on the product molecular weight 452.74. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.5 mM 4.42 mL 22.09 mL 44.18 mL
2.5 mM 0.88 mL 4.42 mL 8.84 mL
5 mM 0.44 mL 2.21 mL 4.42 mL
25 mM 0.09 mL 0.44 mL 0.88 mL

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Product Datasheets for ML 7 hydrochloride

Certificate of Analysis / Product Datasheet
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References for ML 7 hydrochloride

References are publications that support the biological activity of the product.

Saitoh et al (1987) Selective inhibition of catalytic activity of smooth muscle myosin light chain kinase. J.Biol.Chem. 262 7796 PMID: 3108259

Meinders et al (2015) Sp1/Sp3 transcription factors regulate hallmarks of megakaryocyte maturation and platelet formation and function. Blood 125 1957 PMID: 25538045

If you know of a relevant reference for ML 7 hydrochloride, please let us know.

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11 Citations for ML 7 hydrochloride

Citations are publications that use Tocris products. Selected citations for ML 7 hydrochloride include:

Lee et al (2019) Volume expansion and TRPV4 activation regulate stem cell fate in three-dimensional microenvironments. Nat Commun 10 529 PMID: 30705265

Chaudhuri et al (2015) Substrate stress relaxation regulates cell spreading. J Neurosci 6 6364 PMID: 25695512

Bharadwaj (2017) αV-class integrins exert dual roles on α5β1 integrins to strengthen adhesion to fibronectin. Nat Commun 8 14348 PMID: 28128308

Leoni et al (2015) Annexin A1-containing extracellular vesicles and polymeric nanoparticles promote epithelial wound repair. J Clin Invest 125 1215 PMID: 25664854

Do you know of a great paper that uses ML 7 hydrochloride from Tocris? Please let us know.

Reviews for ML 7 hydrochloride

Average Rating: 4.5 (Based on 2 Reviews.)

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Works well.
By Wen Ding on 08/04/2020
Assay Type: In Vivo
Species: Mouse
Cell Line/Tissue: aorta

Mouse from all two groups were housed in condition of a 12 -h shift of light–dark cycle. Mouse of the control group were fed a normal diet, the ML7 group was fed orally treated with ML7 (1 mg/kg daily)

review image

ML7 is good for polarization.
By Anonymous on 09/12/2019
Assay Type: In Vitro
Species: Mouse
Cell Line/Tissue: primary MEO

Bright-field (top) images for tetON-Snai1 MEOs treated with doxycycline and 10 μM ML7 for four days.

review image