ML 297

Pricing Availability   Qty
Description: Selective Kir3.1/3.2 (GIRK1/2) channel activator
Chemical Name: N-(3,4-Difluorophenyl)-N'-(3-methyl-1-phenyl-1H-pyrazol-5-yl)urea
Purity: ≥99% (HPLC)
Reviews (1)

Biological Activity for ML 297

ML 297 is a selective Kir3.1/3.2 (GIRK1/2) channel activator (IC50 values are 160, 887 and 914 nM for GIRK1/2, GIRK1/4 and GIRK1/3 respectively). Exhibits no effect on GIRK2, GIRK2/3, Kir2.1 and Kv7.4 channels, and has minimal effect on a panel of other ion channels, receptors and transporters. Prevents PTZ-induced epileptic seizures in mice. Brain penetrant.

Licensing Information

Sold under license from Vanderbilt University

Compound Libraries for ML 297

ML 297 is also offered as part of the Tocriscreen 2.0 Max. Find out more about compound libraries available from Tocris.

Technical Data for ML 297

M. Wt 328.32
Formula C17H14F2N4O
Storage Store at -20°C
Purity ≥99% (HPLC)
CAS Number 1443246-62-5
PubChem ID 56642816
Smiles O=C(NC2=CC(C)=NN2C3=CC=CC=C3)NC1=CC(F)=C(F)C=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for ML 297

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 32.83 100
ethanol 16.42 50

Preparing Stock Solutions for ML 297

The following data is based on the product molecular weight 328.32. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.05 mL 15.23 mL 30.46 mL
5 mM 0.61 mL 3.05 mL 6.09 mL
10 mM 0.3 mL 1.52 mL 3.05 mL
50 mM 0.06 mL 0.3 mL 0.61 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Dilution Calculator

Calculate the dilution required to prepare a stock solution.

References for ML 297

References are publications that support the biological activity of the product.

Kaufmann et al (2013) ML297 (VU0456810), the first potent and selective activator of the GIRK potassium channel, displays antiepileptic properties in mice. ACS Chem.Neurosci. 4 1278 PMID: 23730969

Wen et al (2013) Discovery of 'molecular switches' within a GIRK activator scaffold that afford selective GIRK inhibitors. Bioorg.Med.Chem.Lett. 23 4562 PMID: 23838260

If you know of a relevant reference for ML 297, please let us know.

View Related Products by Product Action

View all Inward Rectifier Potassium (Kir) Channel Activators

Keywords: ML 297, ML 297 supplier, ML297, selective, Kir3.1, GIRK1, channels, activators, inward, rectifying, rectifiers, potassium, K+, epilepsy, Inward, rectifier, Potassium, Channels, 5380, Tocris Bioscience

Citations for ML 297

Citations are publications that use Tocris products.

Currently there are no citations for ML 297. Do you know of a great paper that uses ML 297 from Tocris? Please let us know.

Reviews for ML 297

Average Rating: 5 (Based on 1 Review.)

5 Star
4 Star
3 Star
2 Star
1 Star

Have you used ML 297?

Submit a review and receive an Amazon gift card.

$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:

ML 297 worked ideally and the data obtained with the product was consistent throughout the study.
By Anonymous on 10/31/2018
Assay Type: In Vitro
Species: Human

ML 297 and ML 277 was used on our University labs to understand potassium channel activation percentage at increasing concentrations. ML 297 worked ideally and the data obtained with the product was consistent throughout the experiment.

review image