(-)-Bicuculline methobromide

Pricing Availability Delivery Time Qty
Cat.No. 0109 - (-)-Bicuculline methobromide | C21H20BrNO6 | CAS No. 73604-30-5
Description: Water-soluble GABAA antagonist
Chemical Name: [R-(R*,S*)]-5-(6,8-Dihydro-8-oxofuro[3,4-e]-1,3-benzodioxol-6-yl)-5,6,7,8-tetrahydro-6,6-dimethyl-1,3-dioxolo[4,5-g]isoquinolinium bromide
Purity: ≥98% (HPLC)
Datasheet
Citations (8)
Literature

Biological Activity

Methobromide salt of (+)-bicuculline (Cat. No. 0130). Water soluble and more stable than bicuculline. Non-GABA receptor-mediated actions reported.

Technical Data

M. Wt 462.3
Formula C21H20BrNO6
Storage Store at RT
Purity ≥98% (HPLC)
CAS Number 73604-30-5
PubChem ID 6852386
InChI Key BWXCECYGGMGBHD-GRTNUQQKSA-M
Smiles [H][C@](C(C=CC6=C5OCO6)=C5C(O4)=O)4[C@]2([H])C1=CC3=C(OCO3)C=C1CC[N+](C)2C.[Br-]

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
water 23.11 50
phosphate buffered saline 46.23 100

Preparing Stock Solutions

The following data is based on the product molecular weight 462.3. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.16 mL 10.82 mL 21.63 mL
5 mM 0.43 mL 2.16 mL 4.33 mL
10 mM 0.22 mL 1.08 mL 2.16 mL
50 mM 0.04 mL 0.22 mL 0.43 mL

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Product Datasheets

Certificate of Analysis / Product Datasheet
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Safety Datasheet

References

References are publications that support the products' biological activity.

Bowery et al (1983) Characteristics of GABAB receptor binding sites on rat whole brain synaptic membranes. Br.J.Pharmacol. 78 191 PMID: 6297646

Seutin and Johnson (1999) Recent advances in the pharmacology of quaternary salts of bicuculline. TiPS 20 268 PMID: 10390643

Sperber et al (1987) Nigral infusions of muscimol or bicuculline facilitate seizures in developing rats. Brain Res. 465 243 PMID: 3440204


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Keywords: Water-soluble GABAA antagonists Receptors GABAA Receptors

8 Citations for (-)-Bicuculline methobromide

Citations are publications that use Tocris products. Selected citations for (-)-Bicuculline methobromide include:

Maccione et al (2014) Following the ontogeny of retinal waves: pan-retinal recordings of population dynamics in the neonatal mouse. J Physiol 592 1545 PMID: 24366261

Maguire et al (2014) Extrasynaptic glycine receptors of rodent dorsal raphe serotonergic neurons: a sensitive target for ethanol. Neuropsychopharmacology 39 1232 PMID: 24264816

Johnson et al (2011) Activation of group II metabotropic glutamate receptors induces long-term depression of excitatory synaptic transmission in the substantia nigra pars reticulata. Neurosci Lett 504 102 PMID: 21945652

Peng et al (2010) Postsynaptic spiking homeostatically induces cell-autonomous regulation of inhibitory inputs via retrograde signaling. J Neurosci 30 16220 PMID: 21123568

Galván et al (2010) Critical involvement of postsynaptic protein kinase activation in long-term potentiation at hippocampal mossy fiber synapses on CA3 interneurons. J Neurosci 30 2844 PMID: 20181582

Sipilä et al (2009) Compensatory enhancement of intrinsic spiking upon NKCC1 disruption in neonatal hippocampus. J Neurosci 29 6982 PMID: 19474325

Krook-Magnuson et al (2008) Tonically active inhibition selectively controls feedforward circuits in mouse barrel cortex. J Neurophysiol 100 932 PMID: 18509076

Huang and Hessler Social modulation during songbird courtship potentiates midbrain dopaminergic neurons. PLoS One 3 e3281 PMID: 18827927


Do you know of a great paper that uses (-)-Bicuculline methobromide from Tocris? If so please let us know.

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