L-Azidohomoalanine hydrochloride

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Description: Unnatural amino acid for bio-orthogonal labeling of newly synthesized proteins
Chemical Name: (S)-2-Amino-4-azidobutanoic acid hydrochloride
Literature (1)

Biological Activity for L-Azidohomoalanine hydrochloride

L-Azidohomoalanine hydrochloride is an unnatural amino acid. Incorporated into proteins during de novo protein synthesis, with no effect on protein degradation. Requires methionine-free medium for efficient incorporation. Substrate for wild-type methionyl-tRNA synthetase (MetRS) in vivo. Can be used in BONCAT (bio-orthogonal non-canonical amino acid tagging) for the identification of newly synthesized proteins.

Technical Data for L-Azidohomoalanine hydrochloride

M. Wt 180.59
Formula C4H8N4O2.HCl
Storage Store at -20°C
CAS Number 942518-29-8
PubChem ID 147480
Smiles N[C@H](C(O)=O)CCN=[N+]=[N-].Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for L-Azidohomoalanine hydrochloride

Solvent Max Conc. mg/mL Max Conc. mM
water 18.06 100

Preparing Stock Solutions for L-Azidohomoalanine hydrochloride

The following data is based on the product molecular weight 180.59. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 5.54 mL 27.69 mL 55.37 mL
5 mM 1.11 mL 5.54 mL 11.07 mL
10 mM 0.55 mL 2.77 mL 5.54 mL
50 mM 0.11 mL 0.55 mL 1.11 mL

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Product Datasheets for L-Azidohomoalanine hydrochloride

Certificate of Analysis / Product Datasheet
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References for L-Azidohomoalanine hydrochloride

References are publications that support the biological activity of the product.

Dieterich et al (2006) Selective identification of newly synthesized proteins in mammalian cells using bioorthogonal noncanonical amino acid tagging (BONCAT). Proc.Natl.Acad.Sci.U.S.A. 103 9482 PMID: 16769897

Lang & Chin et al (2014) Cellular incorporation of unnatural amino acids and bioorthogonal labeling of proteins. Chem.Rev. 114 4764 PMID: 24655057

Wang et al (2017) Nonradioactive quantification of autophagic protein degradation with L-azidohomoalanine labeling. Nat.Protoc. 12 279 PMID: 28079880

If you know of a relevant reference for L-Azidohomoalanine hydrochloride, please let us know.

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Citations for L-Azidohomoalanine hydrochloride

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Fluorescent Dyes and Probes Research Product Guide

Fluorescent Dyes and Probes Research Product Guide

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