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Unnatural amino acid. Incorporated into proteins during de novo protein synthesis, with no effect on protein degradation. Requires methionine-free medium for efficient incorporation. Substrate for wild-type methionyl-tRNA synthetase (MetRS) in vivo. Can be used in BONCAT (bio-orthogonal non-canonical amino acid tagging) for the identification of newly synthesized proteins.
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions
The following data is based on the product molecular weight 180.59. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||5.54 mL||27.69 mL||55.37 mL|
|5 mM||1.11 mL||5.54 mL||11.07 mL|
|10 mM||0.55 mL||2.77 mL||5.54 mL|
|50 mM||0.11 mL||0.55 mL||1.11 mL|
References are publications that support the biological activity of the product.
Dieterich et al (2006) Selective identification of newly synthesized proteins in mammalian cells using bioorthogonal noncanonical amino acid tagging (BONCAT). Proc.Natl.Acad.Sci.U.S.A. 103 9482 PMID: 16769897
Lang & Chin et al (2014) Cellular incorporation of unnatural amino acids and bioorthogonal labeling of proteins. Chem.Rev. 114 4764 PMID: 24655057
Wang et al (2017) Nonradioactive quantification of autophagic protein degradation with L-azidohomoalanine labeling. Nat.Protoc. 12 279 PMID: 28079880
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