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Submit ReviewL-Ascorbic acid
Description: Enhances the generation of iPSCs; increases reprogramming efficiency
Alternative Names: L-Ascorbate, Vitamin C
Chemical Name: 3-Oxo-L-gulofuranolactone
Purity: ≥99% (HPLC)
Biological Activity for L-Ascorbic acid
L-Ascorbic acid is an inhibitor of Cav3.2 channels (IC50 = 6.5 μM); displays no effect on Cav3.1 or Cav3.3 channels heterologously expressed in HEK 293 cells. Also enhances the generation of induced pluripotent stem cells (iPSCs) from mouse and human somatic cells by increasing reprogramming efficiency. Commonly used antifade reagent in live cell microscopy. Naturally occurring antioxidant.
For more information about how L-Ascorbic acid may be used, see our protocol: Highly Efficient Generation of CiPSCs from MEFs
Technical Data for L-Ascorbic acid
| M. Wt | 176.12 |
| Formula | C6H8O6 |
| Storage | Store at RT |
| Purity | ≥99% (HPLC) |
| CAS Number | 50-81-7 |
| PubChem ID | 54670067 |
| InChI Key | CIWBSHSKHKDKBQ-JLAZNSOCSA-N |
| Smiles | OC1=C(O)[C@]([C@@H](O)CO)([H])OC1=O |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for L-Ascorbic acid
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| water | 88.06 | 500 | |
| DMSO | 17.61 | 100 |
Preparing Stock Solutions for L-Ascorbic acid
The following data is based on the product molecular weight 176.12. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 5 mM | 1.14 mL | 5.68 mL | 11.36 mL |
| 25 mM | 0.23 mL | 1.14 mL | 2.27 mL |
| 50 mM | 0.11 mL | 0.57 mL | 1.14 mL |
| 250 mM | 0.02 mL | 0.11 mL | 0.23 mL |
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Product Datasheets for L-Ascorbic acid
References for L-Ascorbic acid
References are publications that support the biological activity of the product.
Padayatty et al (2003) Vitamin C as an antioxidant: evaluation of its role in disease prevention. J.Am.Coll.Nutr. 22 18 PMID: 12569111
Nelson et al (2007) Molecular mechanisms of subtype-specific inhibition of neuronal T-type calcium channels by ascorbate. J.Neurosci. 27 12577 PMID: 18003836
Esteban et al (2010) Vitamin C enhances the generation of mouse and human induced pluripotent stem cells. Cell Stem Cell 6 71 PMID: 20036631
Cordes et al (2011) Mechanisms and advancement of antifading agents for fluorescence microscopy and single-molecule spectroscopy. Phys.Chem.Chem.Phys. 13 6699 PMID: 21311807
If you know of a relevant reference for L-Ascorbic acid, please let us know.
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6 Citations for L-Ascorbic acid
Citations are publications that use Tocris products. Selected citations for L-Ascorbic acid include:
Tristan et al (2021) Robotic high-throughput biomanufacturing and functional differentiation of human pluripotent stem cells. Stem Cell Rep. 16 3076 PMID: 34861164
Guindon et al (2014) Optimization of a cisp. model of chemotherapy-induced peripheral neuropathy in mice: use of vitamin C and sodium bicarbonate pretreatments to reduce nephrotoxicity and improve animal health status. Mol Pain 10 56 PMID: 25189223
Yeligar et al (2009) Ethanol-induced expression of ET-1 and ET-BR in liver sinusoidal endothelial cells and human endothelial cells involves hypoxia-inducible factor-1alpha and microrNA-199. J Immunol 183 5232 PMID: 19783678
Lu et al (2013) Generation of integration-free and region-specific neural progenitors from primate fibroblasts. BMC Dev Biol 3 1580 PMID: 23643533
Shahraz et al (2015) Anti-inflammatory activity of low molecular weight polysialic acid on human macrophages. Cell Rep 5 16800 PMID: 26582367
Wang et al (2018) The COPII cargo adapter SEC24C is essential for neuronal homeostasis. J Clin Invest 128 3319 PMID: 29939162
Do you know of a great paper that uses L-Ascorbic acid from Tocris? Please let us know.
Reviews for L-Ascorbic acid
Average Rating: 5 (Based on 2 Reviews.)
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The hiPSCs were maintained with Essential 8 Flex Medium with L-Ascorbic acid.
By
Anonymous on 02/08/2020
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: ipsc
0.2 mM
iPSC media.
By
Pinyuan Tian on 07/17/2018
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: iPSC
Homemade media for iPSC culture containing L-Ascorbic Acid at a final concentration of 50 ug/ml gives consistent results. Image shows Mapx-5 cell line, containing collagen IV mutations from an Alport Syndrome patient.
Protocols for L-Ascorbic acid
The following protocol features additional information for the use of L-Ascorbic acid (Cat. No. 4055).
Literature in this Area
Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!
*Please note that Tocris will only send literature to established scientific business / institute addresses.
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