L-Ascorbic acid

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Cat.No. 4055 - L-Ascorbic acid | C6H8O6 | CAS No. 50-81-7
Description: Enhances the generation of iPSCs; increases reprogramming efficiency
Alternative Names: L-Ascorbate, Vitamin C
Chemical Name: 3-Oxo-L-gulofuranolactone
Purity: ≥99% (HPLC)
Datasheet
Citations (4)
Reviews (1)
Literature

Biological Activity

Inhibitor of Cav3.2 channels (IC50 = 6.5 μM); displays no effect on Cav3.1 or Cav3.3 channels heterologously expressed in HEK 293 cells. Also enhances the generation of induced pluripotent stem cells (iPSCs) from mouse and human somatic cells by increasing reprogramming efficiency. Naturally occurring antioxidant.

Compound Libraries

L-Ascorbic acid is also offered as part of the Tocriscreen Stem Cell Toolbox. Find out more about compound libraries available from Tocris.

Technical Data

M. Wt 176.12
Formula C6H8O6
Storage Store at RT
Purity ≥99% (HPLC)
CAS Number 50-81-7
PubChem ID 54670067
InChI Key CIWBSHSKHKDKBQ-JLAZNSOCSA-N
Smiles OC1=C(O)[C@]([C@@H](O)CO)([H])OC1=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
water 88.06 500
DMSO 17.61 100

Preparing Stock Solutions

The following data is based on the product molecular weight 176.12. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 5.68 mL 28.39 mL 56.78 mL
5 mM 1.14 mL 5.68 mL 11.36 mL
10 mM 0.57 mL 2.84 mL 5.68 mL
50 mM 0.11 mL 0.57 mL 1.14 mL

Molarity Calculator

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Reconstitution Calculator

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Dilution Calculator

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Product Datasheets

Safety Datasheet

References

References are publications that support the biological activity of the product.

Padayatty et al (2003) Vitamin C as an antioxidant: evaluation of its role in disease prevention. J.Am.Coll.Nutr. 22 18 PMID: 12569111

Nelson et al (2007) Molecular mechanisms of subtype-specific inhibition of neuronal T-type calcium channels by ascorbate. J.Neurosci. 27 12577 PMID: 18003836

Esteban et al (2010) Vitamin C enhances the generation of mouse and human induced pluripotent stem cells. Cell Stem Cell 6 71 PMID: 20036631


If you know of a relevant reference for L-Ascorbic acid, please let us know.

Keywords: L-Ascorbic acid, L-Ascorbic acid supplier, L-Ascorbic, acid, Vc, vitamin, C, ascorbate, calcium, channels, t-type, CaV3.2, selective, Ca2+, channel, blockers, voltage-gated, voltage-dependent, iPSC, stem, cells, L-Ascorbate, Vitamin, Voltage-gated, Calcium, Channels, Reprogramming, Antioxidants, 4055, Tocris Bioscience

4 Citations for L-Ascorbic acid

Citations are publications that use Tocris products. Selected citations for L-Ascorbic acid include:

Guindon et al (2014) Optimization of a cisplatin model of chemotherapy-induced peripheral neuropathy in mice: use of vitamin C and sodium bicarbonate pretreatments to reduce nephrotoxicity and improve animal health status. Mol Pain 10 56 PMID: 25189223

Lu et al (2013) Generation of integration-free and region-specific neural progenitors from primate fibroblasts. BMC Dev Biol 3 1580 PMID: 23643533

Yeligar et al (2009) Ethanol-induced expression of ET-1 and ET-BR in liver sinusoidal endothelial cells and human endothelial cells involves hypoxia-inducible factor-1alpha and microrNA-199. J Immunol 183 5232 PMID: 19783678

Shahraz et al (2015) Anti-inflammatory activity of low molecular weight polysialic acid on human macrophages. Cell Rep 5 16800 PMID: 26582367


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Reviews for L-Ascorbic acid

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iPSC media
By Pinyuan Tian on 07/17/2018
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: iPSC

Homemade media for iPSC culture containing L-Ascorbic Acid at a final concentration of 50 ug/ml gives consistent results. Image shows Mapx-5 cell line, containing collagen IV mutations from an Alport Syndrome patient.

review image

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* or download your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.


Stem Cells

Stem Cells Scientific Review

Written by Kirsty E. Clarke, Victoria B. Christie, Andy Whiting and Stefan A. Przyborski, this review provides an overview of the use of small molecules in the control of stem cell growth and differentiation. Key signaling pathways are highlighted, and the regulation of ES cell self-renewal and somatic cell reprogramming is discussed. Compounds available from Tocris are listed.

Stem Cells

Stem Cells Poster

Written by Rebecca Quelch and Stefan Przyborski from Durham University (UK), this poster describes the isolation of pluripotent stem cells, their maintenance in culture, differentiation, and the generation and potential uses of organoids.