Janelia Fluor® 646, Tetrazine

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Cat.No. 7279 - Janelia Fluor 646, Tetrazine | C38H35N7O3Si | CAS No. 2042192-00-5
Description: Red dye (Reactive group: tetrazine). Applications: super-resolution microscopy e.g. dSTORM & STED, confocal and live cell imaging
Alternative Names: JF646
Chemical Name: 4-((4-(1,2,4,5-Tetrazin-3-yl)benzyl)carbamoyl)-2-(3-(azetidin-1-ium-1-ylidene)-7-(azetidin-1-yl)-5,5-dimethyl-3,5-dihydrodibenzo[b,e]silin-10-yl)benzoate
Purity: ≥90% (HPLC)
Datasheet
Citations
Reviews
Literature (1)

Biological Activity

Red fluorescent dye; supplied with a tetrazine reactive handle for copper-free click chemistry. Suitable for confocal fluorescent imaging, super-resolution microscopy (SRM) techniques such as dSTORM (live and fixed cells) and STED imaging. Can be multiplexed for two-color imaging with Janelia Fluor 549 Tetrazine (Cat. No. 6502). Also suitable for flow cytometry. Cell-permeable. Excitation maximum = 646 nm; emission maximum = 664 nm. Quantum yield = 0.54, Max. extinction coefficient = 152,000 M-1cm-1 (measured in ethanol plus 0.1% TFA); A280 correction factor is 0.19.

Licensing Information

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Optical Data for Janelia Fluor® 646, Tetrazine

Janelia Fluor<sup>®</sup> 646, Tetrazine Dye Spectra
Emission Color Red
Brightness
λabs 646 nm
λem 664 nm
Extinction Coefficient (ε) 152,000 M-1cm-1
Quantum Yield (φ) 0.54
Closest Laser line 647 nm
Reactive Group Tetrazine
Reactivity Copper-free click
Cell Permeable Yes
Correction Factor 260 0.19
Application Confocal imaging, SRM, dSTORM, STED, Flow Cytometry
Alternative For Alexa Fluor® 647, Atto 647, Atto 647N, CF640R, CF647, Cyanine 5

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.

Technical Data

M. Wt 665.83
Formula C38H35N7O3Si
Storage Store at -20°C
Purity ≥90% (HPLC)
CAS Number 2042192-00-5
InChI Key CPWVRCGDSYQFSV-UHFFFAOYSA-N
Smiles C[Si](C)(C1=C/2)C3=CC(N4CCC4)=CC=C3C(C5=CC(C(NCC6=CC=C(C=C6)C7=NN=CN=N7)=O)=CC=C5C([O-])=O)=C1C=CC2=[N+]8CCC/8

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 13.32 20

Preparing Stock Solutions

The following data is based on the product molecular weight 665.83. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.2 mM 7.51 mL 37.55 mL 75.09 mL
1 mM 1.5 mL 7.51 mL 15.02 mL
2 mM 0.75 mL 3.75 mL 7.51 mL
10 mM 0.15 mL 0.75 mL 1.5 mL

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References

References are publications that support the biological activity of the product.

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Keywords: Janelia Fluor 646, Tetrazine, Janelia Fluor 646, Tetrazine supplier, JF646, Janelia, Fluor, 646-SE, fluorescent, dyes, self-labeling, tag, systems, microscopy, cell, permeable, super, resolution, STED, flow, cytometry, SRM, Click, Reactive, Dyes, 7279, Tocris Bioscience

Citations for Janelia Fluor® 646, Tetrazine

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