Janelia Fluor® 646, Tetrazine

Pricing Availability   Qty
Description: Red dye (Reactive group: tetrazine). Application: flow cytometry, confocal microscopy, super-resolution microscopy (SRM) including dSTORM & STED. Suitable for live cell imaging
Alternative Names: JF646
Chemical Name: 4-((4-(1,2,4,5-Tetrazin-3-yl)benzyl)carbamoyl)-2-(3-(azetidin-1-ium-1-ylidene)-7-(azetidin-1-yl)-5,5-dimethyl-3,5-dihydrodibenzo[b,e]silin-10-yl)benzoate
Purity: ≥90% (HPLC)
Citations (1)
Literature (1)

Biological Activity for Janelia Fluor® 646, Tetrazine

Key Information: Janelia Fluor® 646, Tetrazine is a red fluorescent dye; supplied with a tetrazine reactive handle for copper-free click chemistry. Suitable for live cell imaging.

Application: Suitable for flow cytometry, confocal microscopy, super-resolution microscopy (SRM) techniques including dSTORM (in both live and fixed cells) and STED. Can be multiplexed for two-color imaging with Janelia Fluor 549 Tetrazine (Cat. No. 6502). Cell-permeable.

Properties and Photophysical Data: Excitation and emission maxima (λ) are 646 nm and 664 nm, respectively; quantum yield = 0.54; extinction coefficient = 152,000 M-1cm-1 (measured in ethanol plus 0.1% TFA); A280 correction factor = 0.19; lactone-zwitterion equilibrium constant (KL-Z) = 0.0012.

Licensing Information

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Optical Data for Janelia Fluor® 646, Tetrazine

Janelia Fluor<sup>®</sup> 646, Tetrazine Dye Spectra
Emission Color Red
λabs 646 nm
λem 664 nm
Extinction Coefficient (ε) 152,000 M-1cm-1
Quantum Yield (φ) 0.54
Closest Laser line 647 nm
Reactive Group Tetrazine
Reactivity Copper-free click
Cell Permeable Yes
Correction Factor 280 0.19
Application Confocal Microscopy, SRM, dSTORM, STED, Flow Cytometry
Alternative For Alexa Fluor® 647, Atto 647, Atto 647N, CF640R, CF647, Cyanine 5

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.

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Technical Data for Janelia Fluor® 646, Tetrazine

M. Wt 665.83
Formula C38H35N7O3Si
Storage Store at -20°C
Purity ≥90% (HPLC)
CAS Number 2042192-00-5
Smiles C[Si](C)(C1=C/2)C3=CC(N4CCC4)=CC=C3C(C5=CC(C(NCC6=CC=C(C=C6)C7=NN=CN=N7)=O)=CC=C5C([O-])=O)=C1C=CC2=[N+]8CCC/8

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Janelia Fluor® 646, Tetrazine

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 13.32 20

Preparing Stock Solutions for Janelia Fluor® 646, Tetrazine

The following data is based on the product molecular weight 665.83. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.2 mM 7.51 mL 37.55 mL 75.09 mL
1 mM 1.5 mL 7.51 mL 15.02 mL
2 mM 0.75 mL 3.75 mL 7.51 mL
10 mM 0.15 mL 0.75 mL 1.5 mL

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Product Datasheets for Janelia Fluor® 646, Tetrazine

References for Janelia Fluor® 646, Tetrazine

References are publications that support the biological activity of the product.

Zheng et al (2019) Rational design of fluorogenic and spontaneously blinking labels for super-resolution imaging. ACS Cent.Sci. 5 1602 PMID: 31572787

If you know of a relevant reference for Janelia Fluor® 646, Tetrazine, please let us know.

Keywords: Janelia Fluor 646, Tetrazine, Janelia Fluor 646, Tetrazine supplier, JF646, Janelia, Fluor, 646-SE, fluorescent, dyes, self-labeling, tag, systems, microscopy, cell, permeable, super, resolution, STED, flow, cytometry, SRM, Click, Reactive, Dyes, Click-Reactive, Fluorescent, 7279, Tocris Bioscience

1 Citation for Janelia Fluor® 646, Tetrazine

Citations are publications that use Tocris products. Selected citations for Janelia Fluor® 646, Tetrazine include:

Yu et al (2023) Visualizing the disordered nuclear transport machinery in situ. Nature 617 162 PMID: 37100914

Do you know of a great paper that uses Janelia Fluor® 646, Tetrazine from Tocris? Please let us know.

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Fluorescent Dyes and Probes Research Product Guide

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