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Janelia Fluor® 646, Tetrazine
Red fluorescent dye; supplied with a tetrazine reactive handle for copper-free click chemistry. Suitable for confocal fluorescent imaging, super-resolution microscopy (SRM) techniques such as dSTORM (live and fixed cells) and STED imaging. Can be multiplexed for two-color imaging with Janelia Fluor;® 549 Tetrazine (Cat. No. 6502). Also suitable for flow cytometry. Cell-permeable. Excitation maximum = 646 nm; emission maximum = 664 nm. Quantum yield = 0.54, Max. extinction coefficient = 152,000 M-1cm-1 (measured in ethanol plus 0.1% TFA); A280 correction factor is 0.19.
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus
Optical Data for Janelia Fluor® 646, Tetrazine
|Extinction Coefficient (ε)||152,000 M-1cm-1|
|Quantum Yield (φ)||0.54|
|Closest Laser line||647 nm|
|Correction Factor 260||0.19|
|Application||Confocal imaging, SRM, dSTORM, STED, Flow Cytometry|
|Alternative For||Alexa Fluor® 647, Atto 647, Atto 647N, CF640R, CF647, Cyanine 5|
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions
The following data is based on the product molecular weight 665.83. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|0.2 mM||7.51 mL||37.55 mL||75.09 mL|
|1 mM||1.5 mL||7.51 mL||15.02 mL|
|2 mM||0.75 mL||3.75 mL||7.51 mL|
|10 mM||0.15 mL||0.75 mL||1.5 mL|
References are publications that support the biological activity of the product.
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