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Submit ReviewJanelia Fluor® 549, Tetrazine
Description: Yellow dye (Reactive group: tetrazine). Applications: super resolution microscopy e.g. dSTORM & STED, confocal and live cell imaging
Chemical Name: 3,6-Di-1-azetidinyl-9-[[4-[(1,2,4,5-tetrazin-3-yl)benzyl]carbamoyl]-2-carboxyphenyl]xanthylium, inner salt
Biological Activity for Janelia Fluor® 549, Tetrazine
Janelia Fluor® 549, Tetrazine is a yellow fluorescent dye; supplied with a tetrazine reactive handle for copper-free click chemistry. Janelia Fluor® 549 can be coupled directly to the protein of interest with labeling unnatural amino acids by biorthogonal click chemistry in living primary neurons. Suitable for confocal fluorescent imaging and super resolution microscopy (SRM) techniques, such as dSTORM (live and fixed cells) and STED. Cell permeable. Suitable for live-cell imaging. Excitation maximum = 549 nm; emission maximum = 571 nm; Quantum yield = 0.88; Extinction coefficient = 101,000 M-1cm-1; A280 correction factor is 0.169.
Licensing Information
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus
Optical Data for Janelia Fluor® 549, Tetrazine
| Emission Color | Yellow |
|---|---|
| Brightness | |
| λabs | 549 nm |
| λem | 571 nm |
| Extinction Coefficient (ε) | 101,000 M-1cm-1 |
| Quantum Yield (φ) | 0.88 |
| Closest Laser line | 532 nm |
| Reactive Group | Tetrazine |
| Reactivity | Copper-free click |
| Cell Permeable | Yes |
| Correction Factor 280 | 0.169 |
| Application | Confocal imaging, dSTORM, STED |
| Alternative For | Alexa Fluor® 546, Alexa Fluor® 555, BDY TMR-X, Atto 550, CF 555, TAMRA, Cyanine 3 |
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.
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Technical Data for Janelia Fluor® 549, Tetrazine
| M. Wt | 623.66 |
| Formula | C36H29N7O4 |
| Storage | Store at -20°C |
| Smiles | O=C(NCC1=CC=C(C2=NN=CN=N2)C=C1)C3=CC=C(C([O-])=O)C(C(C4=CC=C(N5CCC5)C=C4O6)=C(C=C/7)C6=CC7=[N+]8CCC\8)=C3 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Janelia Fluor® 549, Tetrazine
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 6.24 | 10 |
Preparing Stock Solutions for Janelia Fluor® 549, Tetrazine
The following data is based on the product molecular weight 623.66. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 16.03 mL | 80.17 mL | 160.34 mL |
| 0.5 mM | 3.21 mL | 16.03 mL | 32.07 mL |
| 1 mM | 1.6 mL | 8.02 mL | 16.03 mL |
| 5 mM | 0.32 mL | 1.6 mL | 3.21 mL |
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Product Datasheets for Janelia Fluor® 549, Tetrazine
References for Janelia Fluor® 549, Tetrazine
References are publications that support the biological activity of the product.
Arsic et al (2022) Minimal genetically encoded tags for fluorescent protein labeling in living neurons. Nat.Commun. 13 PMID: 35031604
If you know of a relevant reference for Janelia Fluor® 549, Tetrazine, please let us know.
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1 Citation for Janelia Fluor® 549, Tetrazine
Citations are publications that use Tocris products. Selected citations for Janelia Fluor® 549, Tetrazine include:
Arsic et al (2022) Minimal genetically encoded tags for fluorescent protein labeling in living neurons. Nat.Commun. 13 PMID: 35031604
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Fluorescent Dyes and Probes Research Product GuideUpdated
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