Janelia Fluor® 549, Tetrazine

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Description: Yellow dye (Reactive group: tetrazine). Application: confocal microscopy, super resolution microscopy including dSTORM & STED. Suitable for live cell imaging
Chemical Name: 3,6-Di-1-azetidinyl-9-[[4-[(1,2,4,5-tetrazin-3-yl)benzyl]carbamoyl]-2-carboxyphenyl]xanthylium, inner salt
Citations (2)
Literature (1)

Biological Activity for Janelia Fluor® 549, Tetrazine

Key Information: Janelia Fluor® 549, Tetrazine is a yellow fluorescent dye; supplied with a tetrazine reactive handle for copper-free click chemistry. Suitable for live-cell imaging.

Application: Suitable for confocal microscopy and super resolution microscopy (SRM) techniques including dSTORM (in both live and fixed cells) and STED. Janelia Fluor® 549, tetrazine is cell permeable. It can be coupled directly to a protein of interest using bioorthogonal chemistry and unnatural amino acid technology and has been used in this way to label living primary neurons.

Properties and Photophysical Data: Excitation and emission maxima (λ) are 549 nm and 571 nm, respectively; quantum yield = 0.88; extinction coefficient = 101,000 M-1cm-1; A280 correction factor = 0.169.

Licensing Information

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Optical Data for Janelia Fluor® 549, Tetrazine

Janelia Fluor<sup>®</sup> 549, Tetrazine Dye Spectra
Emission Color Yellow
λabs 549 nm
λem 571 nm
Extinction Coefficient (ε) 101,000 M-1cm-1
Quantum Yield (φ) 0.88
Closest Laser line 532 nm
Reactive Group Tetrazine
Reactivity Copper-free click
Cell Permeable Yes
Correction Factor 280 0.169
Application Confocal Microscopy, dSTORM, STED, Live-cell Imaging
Alternative For Alexa Fluor® 546, Alexa Fluor® 555, BDY TMR-X, Atto 550, CF 555, TAMRA, Cyanine 3

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.

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Technical Data for Janelia Fluor® 549, Tetrazine

M. Wt 623.66
Formula C36H29N7O4
Storage Store at -20°C
Smiles O=C(NCC1=CC=C(C2=NN=CN=N2)C=C1)C3=CC=C(C([O-])=O)C(C(C4=CC=C(N5CCC5)C=C4O6)=C(C=C/7)C6=CC7=[N+]8CCC\8)=C3

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Janelia Fluor® 549, Tetrazine

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 6.24 10

Preparing Stock Solutions for Janelia Fluor® 549, Tetrazine

The following data is based on the product molecular weight 623.66. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.1 mM 16.03 mL 80.17 mL 160.34 mL
0.5 mM 3.21 mL 16.03 mL 32.07 mL
1 mM 1.6 mL 8.02 mL 16.03 mL
5 mM 0.32 mL 1.6 mL 3.21 mL

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Product Datasheets for Janelia Fluor® 549, Tetrazine

References for Janelia Fluor® 549, Tetrazine

References are publications that support the biological activity of the product.

Arsic et al (2022) Minimal genetically encoded tags for fluorescent protein labeling in living neurons. Nat.Commun. 13 PMID: 35031604

If you know of a relevant reference for Janelia Fluor® 549, Tetrazine, please let us know.

Keywords: Janelia Fluor 549, Tetrazine, Janelia Fluor 549, Tetrazine supplier, JF549, Janelia, Fluor, 549,, Fluorescent, dye, tetrazine, microscopy, cell, permeable, super, resolution, SE, STED, Click, Reactive, Dyes, Click-Reactive, Flow, Cytometry, 6502, Tocris Bioscience

2 Citations for Janelia Fluor® 549, Tetrazine

Citations are publications that use Tocris products. Selected citations for Janelia Fluor® 549, Tetrazine include:

Yu et al (2023) Visualizing the disordered nuclear transport machinery in situ. Nature 617 162 PMID: 37100914

Arsic et al (2022) Minimal genetically encoded tags for fluorescent protein labeling in living neurons. Nat.Commun. 13 PMID: 35031604

Do you know of a great paper that uses Janelia Fluor® 549, Tetrazine from Tocris? Please let us know.

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Literature in this Area

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Fluorescent Dyes and Probes Research Product Guide

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