Janelia Fluor® 646, free acid

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Cat.No. 6993 - Janelia Fluor 646, free acid | C29H28N2O4Si.CF3CO2H
Description: Red dye (Reactive group: free acid). Applications: super resolution microscopy e.g. dSTORM & STED, confocal, flow and live cell imaging
Chemical Name: 1-[7-(1-Azetidinyl)-10-(2,5-dicarboxyphenyl)-9,9-dimethyl-9-silaanthracen-2(9H)-ylidene]azetidinium inner salt trifluoroacetate
Purity: ≥95% (HPLC)
Datasheet
Citations
Reviews
Literature (1)

Biological Activity

Red fluorogenic fluorescent dye; supplied as a free acid. Used in protocol (2017 Grimm et al - see references below) for the synthesis of Janelia Fluor® HaloTag® and SNAP-Tag® ligands, for use in live cell imaging experiments. Suitable for confocal fluorescent imaging, super resolution microscopy (SRM) techniques such as dSTORM (live and fixed cells) and STED imaging. Also suitable for flow cytometry. Cell permeable. Excitation maximum = 646 nm; emission maximum = 664 nm; Quantum yield = 0.54; Extinction coefficient = 152,000 M-1cm-1.

To measure the absorbance spectrum of this dye we recommend the following solvent: ethanol or trifluoroethanol plus 0.1% TFA.

We also offer Janelia Fluor® conjugated antibodies and custom conjugation services with our sister company Novus Biologicals.

HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.

Licensing Information

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Optical Data for Janelia Fluor® 646, free acid

Janelia Fluor<sup>®</sup> 646, free acid Dye Spectra
Emission Color Red
Brightness
λabs 646 nm
λem 664 nm
Extinction Coefficient (ε) 152,000 M-1cm-1
Quantum Yield (φ) 0.54
Closest Laser line 640 nm
Reactive Group Free acid
Reactivity Primary amines
Cell Permeable Yes
Correction Factor 280 0.19
Application Confocal imaging, dSTORM, STED, Flow
Alternative For Alexa Fluor® 647, Atto 647, Atto 647N, CF640R, CF647, Cyanine 5

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.

Technical Data

M. Wt 610.65
Formula C29H28N2O4Si.CF3CO2H
Storage Store at -20°C
Purity ≥95% (HPLC)
InChI Key NVBSDKPJAFIPEW-UHFFFAOYSA-N
Smiles OC(C1=CC=C(C([O-])=O)C(C(C2=CC=C(N3CCC3)C=C2[Si]4(C)C)=C(C=C/5)C4=CC5=[N+]6CCC\6)=C1)=O.FC(F)(C(O)=O)F

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMF 12.21 20

Preparing Stock Solutions

The following data is based on the product molecular weight 610.65. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.2 mM 8.19 mL 40.94 mL 81.88 mL
1 mM 1.64 mL 8.19 mL 16.38 mL
2 mM 0.82 mL 4.09 mL 8.19 mL
10 mM 0.16 mL 0.82 mL 1.64 mL

Molarity Calculator

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

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References

References are publications that support the biological activity of the product.

Grimm et al (2017) Synthesis of Janelia Fluor HaloTag and SNAP-Tag ligands and their use in cellular imaging experiments. Methods Mol.Biol. 1663 179 PMID: 28924668

Grimm et al (2015) A general method to improve fluorophores for live-cell and single-molecule microscopy. Nat.Methods 12 244 PMID: 25599551


If you know of a relevant reference for Janelia Fluor® 646, free acid, please let us know.

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Citations for Janelia Fluor® 646, free acid

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