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Janelia Fluor® 646, free acid New
Red fluorogenic fluorescent dye; supplied as a free acid. Used in protocol (2017 Grimm et al - see references below) for the synthesis of Janelia Fluor® HaloTag® and SNAP-Tag® ligands, for use in live cell imaging experiments. Suitable for confocal fluorescent imaging, super resolution microscopy (SRM) techniques such as dSTORM (live and fixed cells) and STED imaging. Also suitable for flow cytometry. Cell permeable. Excitation maximum = 646 nm; emission maximum = 664 nm; Quantum yield = 0.54; Extinction coefficient = 152,000 M-1cm-1.
To measure the absorbance spectrum of this dye we recommend the following solvent: ethanol or trifluoroethanol plus 0.1% TFA.
We also offer Janelia Fluor® conjugated antibodies and custom conjugation services with our sister company Novus Biologicals.
HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus
Optical Data for Janelia Fluor® 646, free acid
|Extinction Coefficient (ε)||152,000 M-1cm-1|
|Quantum Yield (φ)||0.54|
|Closest Laser line||640 nm|
|Reactive Group||Free acid|
|Correction Factor 280||0.19|
|Application||Confocal imaging, dSTORM, STED, Flow|
|Alternative For||Alexa Fluor® 647, Atto 647, Atto 647N, CF640R, CF647, Cyanine 5|
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions
The following data is based on the product molecular weight 610.65. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|0.2 mM||8.19 mL||40.94 mL||81.88 mL|
|1 mM||1.64 mL||8.19 mL||16.38 mL|
|2 mM||0.82 mL||4.09 mL||8.19 mL|
|10 mM||0.16 mL||0.82 mL||1.64 mL|
References are publications that support the biological activity of the product.
Grimm et al (2017) Synthesis of Janelia Fluor HaloTag and SNAP-Tag ligands and their use in cellular imaging experiments. Methods Mol.Biol. 1663 179 PMID: 28924668
Grimm et al (2015) A general method to improve fluorophores for live-cell and single-molecule microscopy. Nat.Methods 12 244 PMID: 25599551
If you know of a relevant reference for Janelia Fluor® 646, free acid, please let us know.
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