Janelia Fluor® 585, BromoCatch™ Ligand

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Description: Fluorogenic Janelia Fluor® 635 conjugated covalent ligand for BromoCatch™, enabling no-wash live-cell imaging
Chemical Name: 4-(((R)-14-((S)-4-(4-Acrylamidophenyl)-2,3,9-trimethyl-6H-thieno[3,2-f][1,2,4]triazolo[4,3-a][1,4]diazepin-6-yl)-13-oxo-3,6,9-trioxa-12-azahexadecyl)carbamoyl)-2-(3-(3,3-difluoroazetidin-1-ium-1-ylidene)-6-(3,3-difluoroazetidin-1-yl)-10,10-dimethyl-3,10-dihydroanthracen-9-yl)benzoate
Purity: ≥95% (HPLC)
Datasheet
Citations
Reviews
Protocols (1)

Biological Activity

Janelia Fluor® 585, BromoCatch™ Ligand is a fluorogenic BromoCatch™ ligand functionalized with Janelia Fluor® 585, designed for real-time, no-wash imaging of BromoCatch-tagged proteins in live cells. Upon covalent binding to the BromoCatch™ tag, this probe undergoes a fluorescence switch-on due to the environmental sensitivity of the Janelia Fluor® 585 dye, delivering bright signal with minimal background fluorescence. Compatible with super-resolution imaging and suitable for dynamic cellular studies, Janelia Fluor® 585, BromoCatch™ Ligand offers a powerful tool for live-cell tracking of BromoCatch-tagged proteins with excellent photostability and imaging contrast.

Please refer to this protocol for further information on how to use this product.

Janelia Fluor® is a registered trademark of Howard Hughes Medical Institute.

BromoCatch™ is a trademark of Bio-Techne Corp.

Licensing Information

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus and the University of Dundee

Technical Data

M. Wt 1172.31
Formula C62H65F4N9O8S
Storage Store at -20°C
Purity ≥95% (HPLC)
InChI Key UKSDBQWJSIWMBF-GFPQFKIWSA-N
Smiles CC1=C(C)SC2=C1C(C3=CC=C(C=C3)NC(C=C)=O)=N[C@@H]([C@H](C(NCCOCCOCCOCCNC(C4=CC=C(C(C5=C6C(C(C)(C)C7=CC(N8CC(F)(F)C8)=CC=C57)=C/C(C=C6)=[N+]9CC(F)(F)C/9)=C4)C([O-])=O)=O)=O)CC)C%10=NN=C(C)N2%10

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

References

References are publications that support the biological activity of the product.

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Citations for Janelia Fluor® 585, BromoCatch™ Ligand

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Protocols for Janelia Fluor® 585, BromoCatch™ Ligand

The following protocol features additional information for the use of Janelia Fluor® 585, BromoCatch™ Ligand (Cat. No. 8998).