Janelia Fluor® 549, free acid

Pricing Availability   Qty
Cat.No. 6503 - Janelia Fluor 549, free acid | C27H22N2O5.C2HF3O2
Description: Yellow fluorescent dye; supplied as a free acid
Chemical Name: 3,6-Di-1-azetidinyl-9-(2,5-dicarboxyphenyl)xanthylium, inner salt trifluoroacetate
Purity: ≥95% (HPLC)
Datasheet
Citations
Reviews
Literature

Biological Activity

Yellow fluorescent dye; supplied as a free acid. Used in protocol (2017 Grimm et al - see references below) for the synthesis of Janelia Fluor® HaloTag® and SNAP-Tag® ligands, for use in live cell imaging experiments. Excitation maximum = 549 nm; emission maximum = 571 nm; Quantum yield = 0.88; Extinction coefficient = 101,000 M-1cm-1.

Licensing Information

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Technical Data

M. Wt 568.5
Formula C27H22N2O5.C2HF3O2
Storage Store at -20°C
Purity ≥95% (HPLC)
Smiles OC(C1=CC=C(C(C(C2=CC=C(C=C2OC3=C/4)N5CCC5)=C3C=CC4=[N+]6CCC/6)=C1)C([O-])=O)=O.FC(F)(F)C(O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 45.45 100

Preparing Stock Solutions

The following data is based on the product molecular weight 568.5. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.76 mL 8.8 mL 17.59 mL
5 mM 0.35 mL 1.76 mL 3.52 mL
10 mM 0.18 mL 0.88 mL 1.76 mL
50 mM 0.04 mL 0.18 mL 0.35 mL

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References

References are publications that support the biological activity of the product.

Grimm et al (2015) A general method to improve fluorophores for live-cell and single-molecule microscopy. Nat. Methods 12 244 PMID: 25599551

Grimm et al (2017) Synthesis of Janelia Fluor HaloTag and SNAP-Tag Ligands and Their Use in Cellular Imaging Experiments. Methods Mol.Biol. 1663 179 PMID: 28924668


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