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Janelia Fluor® 549, free acid
Biological Activity for Janelia Fluor® 549, free acid
Janelia Fluor® 549, free acid is a yellow fluorescent dye; supplied as a free acid. Used in protocol (2017 Grimm et al - see references below) for the synthesis of Janelia Fluor® HaloTag® and SNAP-Tag® ligands, for use in live cell imaging experiments. Also suitable for flow cytometry. Excitation maximum = 549 nm; emission maximum = 571 nm; Quantum yield = 0.88; Extinction coefficient = 101,000 M-1cm-1.
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus
Optical Data for Janelia Fluor® 549, free acid
|Extinction Coefficient (ε)||101,000 M-1cm-1|
|Quantum Yield (φ)||0.88|
|Closest Laser line||532 nm|
|Reactive Group||Free acid|
|Correction Factor 280||0.169|
|Application||Confocal Imaging, dSTORM, STED, Flow Cytometry, Live-cell Imaging|
|Alternative For||Alexa Fluor® 546, Alexa Fluor® 555, BDY TMR-X, Atto 550, CF 555, TAMRA, Cyanine 3|
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.
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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.Spectral Viewer
Technical Data for Janelia Fluor® 549, free acid
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Janelia Fluor® 549, free acid
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions for Janelia Fluor® 549, free acid
The following data is based on the product molecular weight 568.5. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||1.76 mL||8.8 mL||17.59 mL|
|5 mM||0.35 mL||1.76 mL||3.52 mL|
|10 mM||0.18 mL||0.88 mL||1.76 mL|
|50 mM||0.04 mL||0.18 mL||0.35 mL|
Product Datasheets for Janelia Fluor® 549, free acid
References for Janelia Fluor® 549, free acid
References are publications that support the biological activity of the product.
Grimm et al (2015) A general method to improve fluorophores for live-cell and single-molecule microscopy. Nat. Methods 12 244 PMID: 25599551
Grimm et al (2017) Synthesis of Janelia Fluor HaloTag and SNAP-Tag Ligands and Their Use in Cellular Imaging Experiments. Methods Mol.Biol. 1663 179 PMID: 28924668
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Citations for Janelia Fluor® 549, free acid
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Fluorescent Dyes and Probes Research Product Guide
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