You can now submit reviews for your favorite Tocris products. Your review will help other researchers decide on the best products for their research. Why not submit a review today?!Submit Review
Janelia Fluor® 549, free acid
Yellow fluorescent dye; supplied as a free acid. Used in protocol (2017 Grimm et al - see references below) for the synthesis of Janelia Fluor® HaloTag® and SNAP-Tag® ligands, for use in live cell imaging experiments. Excitation maximum = 549 nm; emission maximum = 571 nm; Quantum yield = 0.88; Extinction coefficient = 101,000 M-1cm-1.
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus
Optical Data for Janelia Fluor® 549, free acid
|Extinction Coefficient (ε)||101,000 M-1cm-1|
|Quantum Yield (φ)||0.88|
|Closest Laser line||532 nm|
|Reactive Group||Free acid|
|Correction Factor 280||0.169|
|Application||Confocal imaging, dSTORM, STED, Flow|
|Alternative For||Alexa Fluor® 546, Alexa Fluor® 555, BDY TMR-X, Atto 550, CF 555, TAMRA, Cyanine 3|
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.Spectral Viewer
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions
The following data is based on the product molecular weight 568.5. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||1.76 mL||8.8 mL||17.59 mL|
|5 mM||0.35 mL||1.76 mL||3.52 mL|
|10 mM||0.18 mL||0.88 mL||1.76 mL|
|50 mM||0.04 mL||0.18 mL||0.35 mL|
References are publications that support the biological activity of the product.
Grimm et al (2015) A general method to improve fluorophores for live-cell and single-molecule microscopy. Nat. Methods 12 244 PMID: 25599551
Grimm et al (2017) Synthesis of Janelia Fluor HaloTag and SNAP-Tag Ligands and Their Use in Cellular Imaging Experiments. Methods Mol.Biol. 1663 179 PMID: 28924668
If you know of a relevant reference for Janelia Fluor® 549, free acid, please let us know.
Keywords: Janelia Fluor 549, free acid, Janelia Fluor 549, free acid supplier, JF549, Janelia, Fluor, 549,, Fluorescent, dye, free, acid, microscopy, cell, permeable, super, resolution, SE, STED, halo, tag, snap, Dyes, 6503, Tocris Bioscience
Citations for Janelia Fluor® 549, free acid
Citations are publications that use Tocris products.
Currently there are no citations for Janelia Fluor® 549, free acid. Do you know of a great paper that uses Janelia Fluor® 549, free acid from Tocris? Please let us know.
Reviews for Janelia Fluor® 549, free acid
There are currently no reviews for this product. Be the first to review Janelia Fluor® 549, free acid and earn rewards!
Have you used Janelia Fluor® 549, free acid?
Submit a review and receive an Amazon gift card.
$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Literature in this Area
Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* or download your copy today!
*Please note that Tocris will only send literature to established scientific business / institute addresses.
New Product Guide [Spring/Summer 2019]New
Our new product guide highlights over 215 new products added to the Tocris Bioscience range during the first half of 2019.
- 7-TM Receptors
- Enzyme-Linked Receptors
- Ion Channels
- Nuclear Receptors
- Fluorescent Imaging
- Signal Transduction
- Stem Cells