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Janelia Fluor® 549, Azide
Biological Activity for Janelia Fluor® 549, Azide
Janelia Fluor® 549, Azide is a fluorescent yellow dye; supplied as an azide for click chemisty. Suitable for confocal fluorescent imaging and super resolution microscopy (SRM) techniques, such as dSTORM (live and fixed cells) and STED. Also suitable for flow cytometry. Cell permeable. Excitation maximum = 549 nm; emission maximum = 571 nm; Quantum yield = 0.88; Extinction coefficient = 101,000 M-1cm-1; A280 correction factor is 0.169.
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus
Optical Data for Janelia Fluor® 549, Azide
|Extinction Coefficient (ε)||101,000 M-1cm-1|
|Quantum Yield (φ)||0.88|
|Closest Laser line||532 nm|
|Correction Factor 280||0.169|
|Application||Confocal imaging, dSTORM, STED, Flow|
|Alternative For||Alexa Fluor® 546, Alexa Fluor® 555, BDY TMR-X, Atto 550, CF 555, TAMRA, Cyanine 3|
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.
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Technical Data for Janelia Fluor® 549, Azide
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Janelia Fluor® 549, Azide
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions for Janelia Fluor® 549, Azide
The following data is based on the product molecular weight 654.71. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||1.53 mL||7.64 mL||15.27 mL|
|5 mM||0.31 mL||1.53 mL||3.05 mL|
|10 mM||0.15 mL||0.76 mL||1.53 mL|
|50 mM||0.03 mL||0.15 mL||0.31 mL|
Product Datasheets for Janelia Fluor® 549, Azide
References for Janelia Fluor® 549, Azide
References are publications that support the biological activity of the product.
Grimm et al (2015) A general method to improve fluorophores for live-cell and single-molecule microscopy. Nat.Methods 12 244 PMID: 25599551
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Fluorescent Probes and Dyes Research Product Guide
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