Janelia Fluor® 549, Azide

Discontinued Product

Janelia Fluor® 549, Azide (Cat. No. 6501) has been withdrawn from sale for commercial reasons.
Description: Yellow dye (Reactive group: azide). Applications: super resolution microscopy e.g. dSTORM & STED, confocal, flow and live cell imaging
Chemical Name: 3,6-Di-1-azetidinyl-9-[5-[[2-[2-[2-[2-azidoethoxy]ethoxy]ethoxy]ethyl]carbamoyl]-2-carboxyphenyl]xanthylium, inner salt
Purity: ≥90% (HPLC)
Literature (1)

Biological Activity for Janelia Fluor® 549, Azide

Janelia Fluor® 549, Azide is a fluorescent yellow dye; supplied as an azide for click chemisty. Suitable for confocal fluorescent imaging and super resolution microscopy (SRM) techniques, such as dSTORM (live and fixed cells) and STED. Also suitable for flow cytometry. Cell permeable. Excitation maximum = 549 nm; emission maximum = 571 nm; Quantum yield = 0.88; Extinction coefficient = 101,000 M-1cm-1; A280 correction factor is 0.169.

Licensing Information

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Optical Data for Janelia Fluor® 549, Azide

Janelia Fluor<sup>®</sup> 549, Azide Dye Spectra
Emission Color Yellow
λabs 549 nm
λem 571 nm
Extinction Coefficient (ε) 101,000 M-1cm-1
Quantum Yield (φ) 0.88
Closest Laser line 532 nm
Reactive Group Azide
Reactivity Click
Cell Permeable Yes
Correction Factor 280 0.169
Application Confocal imaging, dSTORM, STED, Flow
Alternative For Alexa Fluor® 546, Alexa Fluor® 555, BDY TMR-X, Atto 550, CF 555, TAMRA, Cyanine 3

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.

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Technical Data for Janelia Fluor® 549, Azide

M. Wt 654.71
Formula C35H38N6O7
Storage Store at -20°C
Purity ≥90% (HPLC)
PubChem ID 137919860
Smiles O=C(NCCOCCOCCOCCN=[N+]=[N-])C1=CC=C(C([O-])=O)C(C(C2=CC=C(N3CCC3)C=C2O4)=C(C=C/5)C4=CC5=[N+]6CCC\6)=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Product Datasheets for Janelia Fluor® 549, Azide

References for Janelia Fluor® 549, Azide

References are publications that support the biological activity of the product.

Grimm et al (2015) A general method to improve fluorophores for live-cell and single-molecule microscopy. Nat.Methods 12 244 PMID: 25599551

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Citations for Janelia Fluor® 549, Azide

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Currently there are no citations for Janelia Fluor® 549, Azide.

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