You can now submit reviews for your favorite Tocris products. Your review will help other researchers decide on the best products for their research. Why not submit a review today?!Submit Review
Janelia Fluor® 549, Azide
Fluorescent dye; supplied as an azide for click chemisty. Suitable for confocal fluorescent imaging and super resolution microscopy (SRM) techniques, such as dSTORM (live and fixed cells) and STED. Cell permeable. Excitation maximum = 549 nm; emission maximum = 571 nm; Quantum yield = 0.88; Extinction coefficient = 101,000 M-1cm-1; A280 correction factor is 0.169.
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus
Janelia Fluor® 549, Azide Dye Spectra
|Extinction coefficient (ε)||101,000 M-1cm-1|
|Quantum yield (φ)||0.88|
|Closest Laser line||532 nm|
|Alternative for||Alexa Fluor® 546, Alexa Fluor® 555, Atto 550, BDY TMR-X, CF555, TAMRA, Cyanine 3|
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.Spectra Viewer
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions
The following data is based on the product molecular weight 654.71. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||1.53 mL||7.64 mL||15.27 mL|
|5 mM||0.31 mL||1.53 mL||3.05 mL|
|10 mM||0.15 mL||0.76 mL||1.53 mL|
|50 mM||0.03 mL||0.15 mL||0.31 mL|
References are publications that support the biological activity of the product.
Grimm et al (2015) A general method to improve fluorophores for live-cell and single-molecule microscopy. Nat.Methods 12 244 PMID: 25599551
If you know of a relevant reference for Janelia Fluor® 549, Azide, please let us know.
Keywords: Janelia Fluor 549, Azide, Janelia Fluor 549, Azide supplier, JF549, Janelia, Fluor, 549,, Fluorescent, dye, azide, microscopy, cell, permeable, super, resolution, SE, STED, Dyes, 6501, Tocris Bioscience
Citations for Janelia Fluor® 549, Azide
Citations are publications that use Tocris products.
Currently there are no citations for Janelia Fluor® 549, Azide. Do you know of a great paper that uses Janelia Fluor® 549, Azide from Tocris? Please let us know.
Reviews for Janelia Fluor® 549, Azide
There are currently no reviews for this product. Be the first to review Janelia Fluor® 549, Azide and earn rewards!
Have you used Janelia Fluor® 549, Azide?
Submit a review and receive an Amazon gift card.
$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Literature in this Area
Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* or download your copy today!
*Please note that Tocris will only send literature to established scientific business / institute addresses.
New Product Guide (Fall/Winter 2018/2019)New
Our new product guide highlights over 190 new products added to the Tocris Bioscience range during the second half of 2018.
- 7-TM Receptors
- Enzyme-Linked Receptors
- Ion Channels
- Nuclear Receptors
- Fluorescent Imaging
- Signal Transduction
- Stem Cells