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Janelia Fluor 526®, SE
Biological Activity for Janelia Fluor 526®, SE
Janelia Fluor 526®, SE is a janelia Fluor 526® is a fluorogenic yellow fluorescent dye; supplied as an NHS ester for coupling to primary amine groups. NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag® and SNAP-tag®. Suitable for confocal fluorescent imaging and super-resolution microscopy (SRM) techniques, such as dSTORM (live and fixed cells) and STED. Can be multiplexed for two color imaging with Janelia Fluor® 635 SE (Cat. No. 6419). Cell permeable. Excitation maximum = 526 nm; emission maximum = 550 nm; quantum yield = 0.87; Extinction coefficient = 118,000 M-1cm-1.
To measure the absorbance spectrum of this dye we recommend the following solvent: TFE plus 0.1% TFA.
Please see the protocol for further information on protein/antibody labeling and conjugation.
We also offer Janelia Fluor® conjugated antibodies and custom conjugation services with our sister company Novus Biologicals.
HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus
Optical Data for Janelia Fluor 526®, SE
|Extinction Coefficient (ε)||118,000 M-1cm-1|
|Quantum Yield (φ)||0.87|
|Closest Laser line||488 nm|
|Reactive Group||NHS ester|
|Application||Confocal Imaging, Super Resolution Microscopy (SRM), Two-color Lattice Light-sheet Microscopy|
|Alternative For||Alexa Fluor® 532, Alexa Fluor® 514, Atto 532, CF514, CF532|
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.Spectral Viewer
Technical Data for Janelia Fluor 526®, SE
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Janelia Fluor 526®, SE
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions for Janelia Fluor 526®, SE
The following data is based on the product molecular weight 659.5. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|0.2 mM||7.58 mL||37.91 mL||75.82 mL|
|1 mM||1.52 mL||7.58 mL||15.16 mL|
|2 mM||0.76 mL||3.79 mL||7.58 mL|
|10 mM||0.15 mL||0.76 mL||1.52 mL|
Product Datasheets for Janelia Fluor 526®, SE
References for Janelia Fluor 526®, SE
References are publications that support the biological activity of the product.
Zheng et al (2019) Rational design of fluorogenic and spontaneously blinking labels for super-resolution imaging. ACS Cent.Sci. 5 1602 PMID: 31572787
If you know of a relevant reference for Janelia Fluor 526®, SE, please let us know.
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Citations for Janelia Fluor 526®, SE
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Protocols for Janelia Fluor 526®, SE
The following protocol features additional information for the use of Janelia Fluor 526®, SE (Cat. No. 7316).
Literature in this Area
Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!
*Please note that Tocris will only send literature to established scientific business / institute addresses.
Fluorescent Dyes and Probes Research Product Guide
This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:
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