Janelia Fluor 526®, SE

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Description: Fluorogenic yellow dye (Reactive group: NHS ester). Application: confocal microscopy, two-color lattice light-sheet microscopy, super resolution microscopy
Alternative Names: JF 526,NHS,JF526
Chemical Name: 2-(3-(3,3-Difluoroazetidin-1-ium-1-ylidene)-6-(3,3-difluoroazetidin-1-yl)-2,7-difluoro-3H-xanthen-9-yl)-4-(((2,5-dioxopyrrolidin-1-yl)oxy)carbonyl)benzoate
Protocols (1)
Literature (1)

Biological Activity for Janelia Fluor 526®, SE

Key Information: Janelia Fluor 526®, SE is a fluorogenic yellow fluorescent dye; supplied with an NHS ester reactive group for the labeling of primary amines.

Application: Suitable for confocal microscopy, two-color lattice light-sheet microscopy, super resolution microscopy (SRM) including dSTORM (in both live and fixed cells) and STED. Cell permeable.

Properties and Photophysical Data: NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag® and SNAP-tag®. Can be multiplexed for two color imaging with Janelia Fluor® 635 SE (Cat. No. 6419). Excitation and emission maxima (λ) are 526 nm and 550 nm, respectively; quantum yield = 0.87; extinction coefficient = 118,000 M-1cm-1; lactone-zwitterion equilibrium constant (KL-Z) = 0.005.

To measure the absorbance spectrum of this dye we recommend the following solvent: TFE plus 0.1% TFA.

Please see the protocol for further information on protein/antibody labeling and conjugation.

We also offer Janelia Fluor® conjugated antibodies and custom conjugation services with our sister company Novus Biologicals.

HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.

Licensing Information

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Optical Data for Janelia Fluor 526®, SE

Janelia Fluor 526<sup>®</sup>, SE Dye Spectra
Emission Color Yellow
λabs 526 nm
λem 550 nm
Extinction Coefficient (ε) 118,000 M-1cm-1
Quantum Yield (φ) 0.87
Closest Laser line 488 nm
Reactive Group NHS ester
Reactivity Primary amines
Cell Permeable Yes
Application Confocal Microscopy, Super Resolution Microscopy (SRM), Two-color Lattice Light-sheet Microscopy
Alternative For Alexa Fluor® 532, Alexa Fluor® 514, Atto 532, CF514, CF532

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectral Viewer

Spectral Viewer

Technical Data for Janelia Fluor 526®, SE

M. Wt 659.5
Formula C31H19F6N3O7
Storage Store at -20°C
Smiles O=C(ON1C(CCC1=O)=O)C2=CC=C(C([O-])=O)C(C(C3=CC(F)=C(N4CC(F)(F)C4)C=C3O5)=C(C=C/6F)C5=CC6=[N+]7CC(F)(F)C\7)=C2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Janelia Fluor 526®, SE

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 13.19 20

Preparing Stock Solutions for Janelia Fluor 526®, SE

The following data is based on the product molecular weight 659.5. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.2 mM 7.58 mL 37.91 mL 75.82 mL
1 mM 1.52 mL 7.58 mL 15.16 mL
2 mM 0.76 mL 3.79 mL 7.58 mL
10 mM 0.15 mL 0.76 mL 1.52 mL

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

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Product Datasheets for Janelia Fluor 526®, SE

References for Janelia Fluor 526®, SE

References are publications that support the biological activity of the product.

Zheng et al (2019) Rational design of fluorogenic and spontaneously blinking labels for super-resolution imaging. ACS Cent.Sci. 5 1602 PMID: 31572787

If you know of a relevant reference for Janelia Fluor 526®, SE, please let us know.

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Citations for Janelia Fluor 526®, SE

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Protocols for Janelia Fluor 526®, SE

The following protocol features additional information for the use of Janelia Fluor 526®, SE (Cat. No. 7316).

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

Fluorescent Dyes and Probes Research Product Guide

Fluorescent Dyes and Probes Research Product Guide

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