Janelia Fluor 526®, SE

Pricing Availability   Qty
Cat.No. 7316 - Janelia Fluor 526, SE | C31H19F6N3O7
Description: Fluorogenic yellow dye; for SMLSM; λabsλem 526/550 nm
Alternative Names: JF 526,NHS, JF526
Chemical Name: 2-(3-(3,3-Difluoroazetidin-1-ium-1-ylidene)-6-(3,3-difluoroazetidin-1-yl)-2,7-difluoro-3H-xanthen-9-yl)-4-(((2,5-dioxopyrrolidin-1-yl)oxy)carbonyl)benzoate
Datasheet
Citations
Reviews
Literature (1)

Biological Activity for Janelia Fluor 526®, SE

Janelia Fluor 526®, SE is a janelia Fluor 526® is a fluorogenic yellow fluorescent dye; supplied as an NHS ester for coupling to primary amine groups. NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag® and SNAP-tag®. Suitable for confocal fluorescent imaging and super-resolution microscopy (SRM) techniques, such as dSTORM (live and fixed cells) and STED. Can be multiplexed for two color imaging with Janelia Fluor® 635 SE (Cat. No. 6419). Cell permeable. Excitation maximum = 526 nm; emission maximum = 550 nm; quantum yield = 0.87; Extinction coefficient = 118,000 M-1cm-1.

To measure the absorbance spectrum of this dye we recommend the following solvent: TFE plus 0.1% TFA.

We also offer Janelia Fluor® conjugated antibodies and custom conjugation services with our sister company Novus Biologicals.

HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.

Licensing Information

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Optical Data for Janelia Fluor 526®, SE

Janelia Fluor 526<sup>®</sup>, SE Dye Spectra
Emission Color Yellow
Brightness
λabs 526 nm
λem 550 nm
Extinction Coefficient (ε) 118,000 M-1cm-1
Quantum Yield (φ) 0.87
Closest Laser line 488 nm
Reactive Group NHS ester
Reactivity Primary amines
Cell Permeable Yes
Application Confocal imaging, Super resolution microscopy, two-color lattice light-sheet microscopy
Alternative For Alexa Fluor® 532, Alexa Fluor® 514, Atto 532, CF514, CF532

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectral Viewer

Spectral Viewer

Technical Data for Janelia Fluor 526®, SE

M. Wt 659.5
Formula C31H19F6N3O7
Storage Store at -20°C
InChI Key BCNDQOPFHGWJEL-UHFFFAOYSA-N
Smiles O=C(ON1C(CCC1=O)=O)C2=CC=C(C([O-])=O)C(C(C3=CC(F)=C(N4CC(F)(F)C4)C=C3O5)=C(C=C/6F)C5=CC6=[N+]7CC(F)(F)C\7)=C2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Janelia Fluor 526®, SE

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 13.19 20

Preparing Stock Solutions for Janelia Fluor 526®, SE

The following data is based on the product molecular weight 659.5. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.2 mM 7.58 mL 37.91 mL 75.82 mL
1 mM 1.52 mL 7.58 mL 15.16 mL
2 mM 0.76 mL 3.79 mL 7.58 mL
10 mM 0.15 mL 0.76 mL 1.52 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.
=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Dilution Calculator

Calculate the dilution required to prepare a stock solution.
x
=
x

Product Datasheets for Janelia Fluor 526®, SE

References for Janelia Fluor 526®, SE

References are publications that support the biological activity of the product.

Zheng et al (2019) Rational design of fluorogenic and spontaneously blinking labels for super-resolution imaging. ACS Cent.Sci. 5 1602 PMID: 31572787


If you know of a relevant reference for Janelia Fluor 526®, SE, please let us know.

Keywords: Janelia Fluor 526, SE, Janelia Fluor 526, SE supplier, JF526, Janelia, Fluor, 526,, SE, fluorogenic, yellow, dye, Fluorescent, NHS, ester, self-labeling, tag, systems, super, resolution, microscopy, cell, permeable, JF, 526,NHS, Amine, Reactive, Dyes, 7316, Tocris Bioscience

Citations for Janelia Fluor 526®, SE

Citations are publications that use Tocris products.

Currently there are no citations for Janelia Fluor 526®, SE. Do you know of a great paper that uses Janelia Fluor 526®, SE from Tocris? Please let us know.

Reviews for Janelia Fluor 526®, SE

There are currently no reviews for this product. Be the first to review Janelia Fluor 526®, SE and earn rewards!

Have you used Janelia Fluor 526®, SE?

Submit a review and receive an Amazon gift card.

$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.


Fluorescent Probes and Dyes

Fluorescent Probes and Dyes Research Product Guide

This product guide provides a comprehensive list and background to the use of Fluorescent Probes and Dyes

  • Fluorescent Dyes
  • Dyes for Flow Cytometry
  • Fluorescent Probes
  • Anti-fade Reagents
  • Bioluminescent Substrates
  • Fluorogenic Dyes for Light-Up Aptamers
  • Fluorescent Probes for Imaging Bacteria
  • TSA Reagents for Enhancing IHC, ICC & FISH Signals