Home / Product Type / Fluorescent Imaging / Fluorescent Dyes / Janelia Fluor® Dyes / Janelia Fluor® 635, SE

Janelia Fluor® 635, SE

Cat. No. 6419 Submit a Review Datasheet / COA / SDS
Pricing Availability   Qty
Cat.No. 6419 - Janelia Fluor 635, SE | C33H29F2N3O6Si
Description: Cell permeable red fluorescent dye; supplied as NHS ester
Alternative Names: JF 635, NHS
Chemical Name: 1-[10-[2-Carboxy-5-[[(2,5-dioxo-1-pyrrolidinyl)oxy]carbonyl]phenyl]-9,9-dimethyl-7-[1-(3-fluoroazetidinyl)]-9-silaanthracen-2(9H)-ylidene]-3-fluoroazetidinium, inner salt
Datasheet
Citations
Reviews
Protocols (1)
Literature
Biological Activity Technical Data Solubility Calculators Datasheets References

Biological Activity

Red fluorescent dye; supplied as an NHS ester for coupling to primary amine groups. NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag® and SNAP-tag®. Suitable for confocal fluorescent imaging and super resolution microscopy (SRM) techniques, such as dSTORM (live and fixed cells). Cell permeable. Excitation maximum = 635 nm; emission maximum = 652 nm; Quantum yield = 0.56; Max extinction coefficient = 167,000 M-1cm-1 (measured in Ethanol or TFE plus 0.1% TFA); Correction factor = 0.0524. Exhibits low nonspecific background staining.

To measure the absorbance spectrum of this dye we recommend the following solvent: ethanol or trifluoroethanol plus 0.1% TFA.

We also offer Janelia Fluo® conjugated antibodies and custom conjugation services with our sister company Novus Biologicals.

HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.

Licensing Information

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Application of Janelia Fluor® 635, SE dye on Mitochondria

Janelia Fluor® 635, SE Dye-labeled Mitochondria Janelia Fluor® 635, SE Dye-labeled Mitochondria

Application of Janelia Fluor® 635, SE dye on mitochondria; Left: Mitochondria were labeled with anti-TOM20 primary antibodies. The secondary antibodies were conjugated to JF 635 dye Left: Maximum intensity projection of confocal z-stack, recorded on an Olympus FV1200 confocal microscope. Right: Enlarged portion of left-hand image.

Images kindly provided by Dr Kirstin Elgass, Monash University

Janelia Fluor® 635, SE Dye Spectra

Spectra charts

Technical Data

M. Wt 629.69
Formula C33H29F2N3O6Si
Storage Store at -20°C
Smiles O=C(ON1C(CCC1=O)=O)C2=CC=C(C([O-])=O)C(C(C3=CC=C(N4CC(F)C4)C=C3[Si]5(C)C)=C(C=C/6)C5=CC6=[N+]7CC(F)C\7)=C2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 62.97 100

Preparing Stock Solutions

The following data is based on the product molecular weight 629.69. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.59 mL 7.94 mL 15.88 mL
5 mM 0.32 mL 1.59 mL 3.18 mL
10 mM 0.16 mL 0.79 mL 1.59 mL
50 mM 0.03 mL 0.16 mL 0.32 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.
=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Dilution Calculator

Calculate the dilution required to prepare a stock solution.
x
=
x

References

References are publications that support the biological activity of the product.

Grimm et al (2017) A general method to fine-tune fluorophores for live-cell and in vivo imaging. Nat.Methods 14 987 PMID: 28869757

If you know of a relevant reference for Janelia Fluor® 635, SE, please let us know.

View Related Products by Target

Keywords: Janelia Fluor 635, SE, Janelia Fluor 635, SE supplier, JF635, Janelia, Fluor, 635-SE, fluorescent, dyes, NHS, ester, self-labeling, tag, systems, microscopy, cell, permeable, super, resolution, JF, 635,, Dyes, 6419, Tocris Bioscience

Citations for Janelia Fluor® 635, SE

Citations are publications that use Tocris products.

Currently there are no citations for Janelia Fluor® 635, SE. Do you know of a great paper that uses Janelia Fluor® 635, SE from Tocris? Please let us know.

Reviews for Janelia Fluor® 635, SE

There are currently no reviews for this product. Be the first to review Janelia Fluor® 635, SE and earn rewards!

Have you used Janelia Fluor® 635, SE?

Submit a review and receive an Amazon gift card.

$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Protocols for Janelia Fluor® 635, SE

The following protocol features additional information for the use of Janelia Fluor® 635, SE (Cat. No. 6419).

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* or download your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

New Product Guide

New Product Guide (Spring/Summer 2018)

Our new product guide highlights over 200 new products added to the Tocris Bioscience range during the second half of 2017.

  • 7-TM Receptors
  • Enzymes
  • Enzyme-Linked Receptors
  • Ion Channels
  • Nuclear Receptors
  • Transporters
  • Aptamers
  • Cell Metabolism
  • Epigenetics
  • Fluorescent Imaging
  • Signal Transduction
  • Stem Cells