ISO 1

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Cat.No. 4288 - ISO 1 | C12H13NO4 | CAS No. 478336-92-4
Description: Macrophage migration inhibitory factor (MIF) inhibitor
Chemical Name: 4,5-Dihydro-3-(4-hydroxyphenyl)-5-isoxazoleacetic acid methyl ester
Purity: ≥98% (HPLC)
Datasheet
Citations (1)
Reviews
Literature

Biological Activity

Macrophage migration inhibitory factor (MIF) inhibitor (IC50 = 7 μM); inhibits MIF tautomerase activity in vitro and in vivo. Protective against mouse models of streptozotocin-induced diabetes mellitus; blocks anti-inflammatory response following LPS exposure and increases survival. Inhibits airway remodeling in a mouse model of chronic asthma. Cell permeable; orally bioavailable.

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Technical Data

M. Wt 235.24
Formula C12H13NO4
Storage Store at +4°C
Purity ≥98% (HPLC)
CAS Number 478336-92-4
PubChem ID 6098948
InChI Key CLVQGFPQFNASJH-UHFFFAOYSA-N
Smiles OC(C=C2)=CC=C2C1=NOC(CC(OC)=O)C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 23.52 100
ethanol 23.52 100

Preparing Stock Solutions

The following data is based on the product molecular weight 235.24. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 4.25 mL 21.25 mL 42.51 mL
5 mM 0.85 mL 4.25 mL 8.5 mL
10 mM 0.43 mL 2.13 mL 4.25 mL
50 mM 0.09 mL 0.43 mL 0.85 mL

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References

References are publications that support the biological activity of the product.

Al-Abed et al (2005) ISO-1 binding to the tautomerase active site of MIF inhibits its pro-inflammatory activity and increases survival in severe sepsis. J.Biol.Chem. 280 36541 PMID: 16115897

Cvetkovic et al (2005) Critical role of macrophage migration inhibitory factor activity in experimental autoimmune diabetes. Endocrinology 146 2942 PMID: 15790730

Chen et al (2010) ISO-1, a macrophage migration inhibitory factor antagonist, inhibits airway remodeling in a murine model of chronic asthma. Mol.Med. 16 400 PMID: 20485865

Lubetsky et al (2002) The tautomerase active site of macrophage migration inhibitory factor is a potential target for discovery of novel anti-inflammatory agents. J.Biol.Chem. 277 24976 PMID: 11997397


If you know of a relevant reference for ISO 1, please let us know.

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Keywords: ISO 1, ISO 1 supplier, ISO1, macrophage, migration, inhibitory, factor, antagonists, inhibits, inhibitors, tautomerase, MIF, Cytokines, 4288, Tocris Bioscience

1 Citation for ISO 1

Citations are publications that use Tocris products. Selected citations for ISO 1 include:

Graham et al (2015) The expression of microRNA-451 in human endometriotic lesions is inversely related to that of macrophage migration inhibitory factor (MIF) and regulates MIF expression and modulation of epithelial cell survival. Hum Reprod 30 642 PMID: 25637622


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