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Biological Activity for iCRT 14
iCRT 14 is a potent inhibitor of β-catenin-responsive transcription (CRT) (IC50 = 40.3 nM in assays of Wnt pathway activity). Thought to directly influence the interaction between β-catenin and TCF4. Induces marked G0/G1 cell cycle arrest in HCT-116 and HT29 cell lines. Also acts as transfection enhancer; enhances transgene expression in PC-3 cells by 16 to 35-fold using nonviral systems.
Compound Libraries for iCRT 14
Technical Data for iCRT 14
|Storage||Store at +4°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for iCRT 14
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions for iCRT 14
The following data is based on the product molecular weight 375.44. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|0.75 mM||3.55 mL||17.76 mL||35.51 mL|
|3.75 mM||0.71 mL||3.55 mL||7.1 mL|
|7.5 mM||0.36 mL||1.78 mL||3.55 mL|
|37.5 mM||0.07 mL||0.36 mL||0.71 mL|
References for iCRT 14
References are publications that support the biological activity of the product.
Gonsalves et al (2011) An RNAi-based chemical genetic screen identifies three small-molecule inhibitors of the Wnt/wingless signaling pathway. Proc.Natl.Acad.Sci.USA 108 5954 PMID: 21393571
Watanabe and Dai (2011) Winning WNT: race to Wnt signaling inhibitors. Proc.Natl.Acad.Sci.USA 108 5929 PMID: 21451134
Spivack et al (2021) Enhancement of transgene expression by the β-catenin inhibitor iCRT14. Plasmid PMID: 33472046
If you know of a relevant reference for iCRT 14, please let us know.
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9 Citations for iCRT 14
Citations are publications that use Tocris products. Selected citations for iCRT 14 include:
Kennedy et al (2016) Sp5 and Sp8 recruit β-catenin and Tcf1-Lef1 to select enhancers to activate Wnt target gene transcription. Proc Natl Acad Sci U S A 113 3545 PMID: 26969725
Sarah J et al (2023) Pro-inflammatory role of Wnt/β-catenin signaling in endothelial dysfunction. Front Cardiovasc Med 9 1059124 PMID: 36794234
Paula et al (2020) Developmentally Programmed Tankyrase Activity Upregulates β-Catenin and Licenses Progression of Embryonic Genome Activation. Dev Cell 53 545-560.e7 PMID: 32442396
Wu et al (2012) Differential modulation of the oligodendrocyte transcriptome by sonic hedgehog and bone morphogenetic protein 4 via opposing effects on histone acetylation. J Neurosci 32 6651 PMID: 22573687
Volpini et al (2018) Trypanosoma cruzi Exploits Wnt Signaling Pathway to Promote Its Intracellular Replication in Macrophages. Front Immunol 9 859 PMID: 29743880
Gowda et al (2018) Mutant Isocitrate Dehydrogenase 1 Disrupts PKM2-β-Catenin-BRG1 Transcriptional Network-Driven CD47 Expression. Mol Cell Biol 38 PMID: 29463646
Ling et al (2021) Commitment and oncogene-induced plasticity of human stem cell-derived pancreatic acinar and ductal organoids. Cell Stem Cell 28 1090-1104.e6 PMID: 33915081
Alex M et al (2021) Heparanase-Induced Activation of AKT Stabilizes β-Catenin and Modulates Wnt/β-Catenin Signaling during Herpes Simplex Virus 1 Infection. MBio 12 e0279221 PMID: 34749529
Brown et al (2019) Aging differentially modulates the Wnt pro-survival signalling pathways in vascular smooth muscle cells. Aging Cell 18 e12844 PMID: 30548452
Do you know of a great paper that uses iCRT 14 from Tocris? Please let us know.
Reviews for iCRT 14
Average Rating: 5 (Based on 1 Review.)
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Literature in this Area
Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!
*Please note that Tocris will only send literature to established scientific business / institute addresses.