iCRT 14

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Cat.No. 4299 - iCRT 14 | C21H17N3O2S | CAS No. 677331-12-3
Description: Inhibits β-catenin-responsive transcription (CRT)
Chemical Name: 5-[[2,5-Dimethyl-1-(3-pyridinyl)-1H-pyrrol-3-yl]methylene]-3-phenyl-2,4-thiazolidinedion
Purity: ≥99% (HPLC)
Datasheet
Citations (5)
Reviews
Literature (1)
Pathways (1)

Biological Activity

Potent inhibitor of β-catenin-responsive transcription (CRT) (IC50 = 40.3 nM in assays of Wnt pathway activity). Thought to directly influence the interaction between β-catenin and TCF4. Induces marked G0/G1 cell cycle arrest in HCT-116 and HT29 cell lines.

Compound Libraries

iCRT 14 is also offered as part of the Tocriscreen Plus. Find out more about compound libraries available from Tocris.

Technical Data

M. Wt 375.44
Formula C21H17N3O2S
Storage Store at +4°C
Purity ≥99% (HPLC)
CAS Number 677331-12-3
PubChem ID 5967294
InChI Key NCSHZXNGQYSKLR-UNOMPAQXSA-N
Smiles CC(N(C4=CN=CC=C4)C(C)=C3)=C3/C=C(SC2=O)/C(N2C1=CC=CC=C1)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 28.16 75

Preparing Stock Solutions

The following data is based on the product molecular weight 375.44. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.75 mM 3.55 mL 17.76 mL 35.51 mL
3.75 mM 0.71 mL 3.55 mL 7.1 mL
7.5 mM 0.36 mL 1.78 mL 3.55 mL
37.5 mM 0.07 mL 0.36 mL 0.71 mL

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References

References are publications that support the biological activity of the product.

Gonsalves et al (2011) An RNAi-based chemical genetic screen identifies three small-molecule inhibitors of the Wnt/wingless signaling pathway. Proc.Natl.Acad.Sci.USA 108 5954

Watanabe and Dai (2011) Winning WNT: race to Wnt signaling inhibitors. Proc.Natl.Acad.Sci.USA 108 5929


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View Related Products by Target

Keywords: iCRT 14, iCRT 14 supplier, iCRT14, beta-catenin, b-catenin, β-catenin, catenin-responsive, transcription, crt, inhibitors, inhibits, wnt, signaling, signalling, TCF4, Beta-catenin, 4299, Tocris Bioscience

5 Citations for iCRT 14

Citations are publications that use Tocris products. Selected citations for iCRT 14 include:

Brown et al (2019) Aging differentially modulates the Wnt pro-survival signalling pathways in vascular smooth muscle cells. Aging Cell 18 e12844 PMID: 30548452

Kennedy et al (2016) Sp5 and Sp8 recruit β-catenin and Tcf1-Lef1 to select enhancers to activate Wnt target gene transcription. Proc Natl Acad Sci U S A 113 3545 PMID: 26969725

Volpini et al (2018) Trypanosoma cruzi Exploits Wnt Signaling Pathway to Promote Its Intracellular Replication in Macrophages. Front Immunol 9 859 PMID: 29743880

Gowda et al (2018) Mutant Isocitrate Dehydrogenase 1 Disrupts PKM2-β-Catenin-BRG1 Transcriptional Network-Driven CD47 Expression. Mol Cell Biol 38 PMID: 29463646

Wu et al (2012) Differential modulation of the oligodendrocyte transcriptome by sonic hedgehog and bone morphogenetic protein 4 via opposing effects on histone acetylation. J Neurosci 32 6651 PMID: 22573687


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Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* or download your copy today!

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Cancer

Cancer Research Product Guide

A collection of over 750 products for cancer research, the guide includes research tools for the study of:

  • Cancer Metabolism
  • Epigenetics in Cancer
  • Receptor Signaling
  • Cell Cycle and DNA Damage Repair
  • Angiogenesis
  • Invasion and Metastasis

Pathways for iCRT 14

Wnt

Wnt Signaling Pathway

The Wnt pathway is involved in cellular differentiation and proliferation in adult tissues and also during embryogenesis. Disturbances within the pathway may lead to the formation of tumors and promote metastasis.