iCRT 14

Pricing Availability   Qty
Description: Inhibits β-catenin-responsive transcription (CRT)
Chemical Name: 5-[[2,5-Dimethyl-1-(3-pyridinyl)-1H-pyrrol-3-yl]methylene]-3-phenyl-2,4-thiazolidinedion
Purity: ≥99% (HPLC)
Datasheet
Citations (9)
Reviews (1)
Pathways (1)

Biological Activity for iCRT 14

iCRT 14 is a potent inhibitor of β-catenin-responsive transcription (CRT) (IC50 = 40.3 nM in assays of Wnt pathway activity). Thought to directly influence the interaction between β-catenin and TCF4. Induces marked G0/G1 cell cycle arrest in HCT-116 and HT29 cell lines. Also acts as transfection enhancer; enhances transgene expression in PC-3 cells by 16 to 35-fold using nonviral systems.

Compound Libraries for iCRT 14

iCRT 14 is also offered as part of the Tocriscreen 2.0 Max. Find out more about compound libraries available from Tocris.

Technical Data for iCRT 14

M. Wt 375.44
Formula C21H17N3O2S
Storage Store at +4°C
Purity ≥99% (HPLC)
CAS Number 677331-12-3
PubChem ID 5967294
InChI Key NCSHZXNGQYSKLR-UNOMPAQXSA-N
Smiles CC(N(C4=CN=CC=C4)C(C)=C3)=C3/C=C(SC2=O)/C(N2C1=CC=CC=C1)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for iCRT 14

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 28.16 75

Preparing Stock Solutions for iCRT 14

The following data is based on the product molecular weight 375.44. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.75 mM 3.55 mL 17.76 mL 35.51 mL
3.75 mM 0.71 mL 3.55 mL 7.1 mL
7.5 mM 0.36 mL 1.78 mL 3.55 mL
37.5 mM 0.07 mL 0.36 mL 0.71 mL

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References for iCRT 14

References are publications that support the biological activity of the product.

Gonsalves et al (2011) An RNAi-based chemical genetic screen identifies three small-molecule inhibitors of the Wnt/wingless signaling pathway. Proc.Natl.Acad.Sci.USA 108 5954 PMID: 21393571

Watanabe and Dai (2011) Winning WNT: race to Wnt signaling inhibitors. Proc.Natl.Acad.Sci.USA 108 5929 PMID: 21451134

Spivack et al (2021) Enhancement of transgene expression by the β-catenin inhibitor iCRT14. Plasmid PMID: 33472046


If you know of a relevant reference for iCRT 14, please let us know.

Keywords: iCRT 14, iCRT 14 supplier, iCRT14, beta-catenin, b-catenin, β-catenin, catenin-responsive, transcription, crt, inhibitors, inhibits, wnt, signaling, signalling, TCF4, transfection, enhancers, enhances, transgene, expression, plasmid, DNA, polyplex, Beta-catenin, Biochemicals, and, Molecular, Biology, 4299, Tocris Bioscience

9 Citations for iCRT 14

Citations are publications that use Tocris products. Selected citations for iCRT 14 include:

Kennedy et al (2016) Sp5 and Sp8 recruit β-catenin and Tcf1-Lef1 to select enhancers to activate Wnt target gene transcription. Proc Natl Acad Sci U S A 113 3545 PMID: 26969725

Sarah J et al (2023) Pro-inflammatory role of Wnt/β-catenin signaling in endothelial dysfunction. Front Cardiovasc Med 9 1059124 PMID: 36794234

Paula et al (2020) Developmentally Programmed Tankyrase Activity Upregulates β-Catenin and Licenses Progression of Embryonic Genome Activation. Dev Cell 53 545-560.e7 PMID: 32442396

Wu et al (2012) Differential modulation of the oligodendrocyte transcriptome by sonic hedgehog and bone morphogenetic protein 4 via opposing effects on histone acetylation. J Neurosci 32 6651 PMID: 22573687


Do you know of a great paper that uses iCRT 14 from Tocris? Please let us know.

Reviews for iCRT 14

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Useful Wnt inhibitor.
By Anonymous on 07/11/2020
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: HCT116

HCT116 cells were treated with different concentrations of iCRT-14 for 24 hours and the cells were used for transwell assays to investigate the effects of Wnt inhibitor on cell migration.

review image

Pathways for iCRT 14

Wnt Signaling Pathway

Wnt Signaling Pathway

The Wnt pathway is involved in cellular differentiation and proliferation in adult tissues and also during embryogenesis. Disturbances within the pathway may lead to the formation of tumors and promote metastasis.