iCRT 14

Pricing Availability   Qty
Cat.No. 4299 - iCRT 14 | C21H17N3O2S | CAS No. 677331-12-3
Description: Inhibits β-catenin-responsive transcription (CRT)
Chemical Name: 5-[[2,5-Dimethyl-1-(3-pyridinyl)-1H-pyrrol-3-yl]methylene]-3-phenyl-2,4-thiazolidinedion
Purity: ≥99% (HPLC)
Datasheet
Citations (5)
Reviews (1)
Literature (1)
Pathways (1)

Biological Activity for iCRT 14

iCRT 14 is a potent inhibitor of β-catenin-responsive transcription (CRT) (IC50 = 40.3 nM in assays of Wnt pathway activity). Thought to directly influence the interaction between β-catenin and TCF4. Induces marked G0/G1 cell cycle arrest in HCT-116 and HT29 cell lines. Also acts as transfection enhancer; enhances transgene expression in PC-3 cells by 16 to 35-fold using nonviral systems.

Compound Libraries for iCRT 14

iCRT 14 is also offered as part of the Tocriscreen 2.0 Max. Find out more about compound libraries available from Tocris.

Technical Data for iCRT 14

M. Wt 375.44
Formula C21H17N3O2S
Storage Store at +4°C
Purity ≥99% (HPLC)
CAS Number 677331-12-3
PubChem ID 5967294
InChI Key NCSHZXNGQYSKLR-UNOMPAQXSA-N
Smiles CC(N(C4=CN=CC=C4)C(C)=C3)=C3/C=C(SC2=O)/C(N2C1=CC=CC=C1)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for iCRT 14

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 28.16 75

Preparing Stock Solutions for iCRT 14

The following data is based on the product molecular weight 375.44. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.75 mM 3.55 mL 17.76 mL 35.51 mL
3.75 mM 0.71 mL 3.55 mL 7.1 mL
7.5 mM 0.36 mL 1.78 mL 3.55 mL
37.5 mM 0.07 mL 0.36 mL 0.71 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.
=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Dilution Calculator

Calculate the dilution required to prepare a stock solution.
x
=
x

References for iCRT 14

References are publications that support the biological activity of the product.

Gonsalves et al (2011) An RNAi-based chemical genetic screen identifies three small-molecule inhibitors of the Wnt/wingless signaling pathway. Proc.Natl.Acad.Sci.USA 108 5954

Watanabe and Dai (2011) Winning WNT: race to Wnt signaling inhibitors. Proc.Natl.Acad.Sci.USA 108 5929

Spivack et al (2021) Enhancement of transgene expression by the β-catenin inhibitor iCRT14. Plasmid PMID: 33472046


If you know of a relevant reference for iCRT 14, please let us know.

Keywords: iCRT 14, iCRT 14 supplier, iCRT14, beta-catenin, b-catenin, β-catenin, catenin-responsive, transcription, crt, inhibitors, inhibits, wnt, signaling, signalling, TCF4, transfection, enhancers, enhances, transgene, expression, plasmid, DNA, polyplex, Beta-catenin, Biochemicals, and, Molecular, Biology, 4299, Tocris Bioscience

5 Citations for iCRT 14

Citations are publications that use Tocris products. Selected citations for iCRT 14 include:

Kennedy et al (2016) Sp5 and Sp8 recruit β-catenin and Tcf1-Lef1 to select enhancers to activate Wnt target gene transcription. Proc Natl Acad Sci U S A 113 3545 PMID: 26969725

Brown et al (2019) Aging differentially modulates the Wnt pro-survival signalling pathways in vascular smooth muscle cells. Aging Cell 18 e12844 PMID: 30548452

Volpini et al (2018) Trypanosoma cruzi Exploits Wnt Signaling Pathway to Promote Its Intracellular Replication in Macrophages. Front Immunol 9 859 PMID: 29743880

Gowda et al (2018) Mutant Isocitrate Dehydrogenase 1 Disrupts PKM2-β-Catenin-BRG1 Transcriptional Network-Driven CD47 Expression. Mol Cell Biol 38 PMID: 29463646


Do you know of a great paper that uses iCRT 14 from Tocris? Please let us know.

Reviews for iCRT 14

Average Rating: 5 (Based on 1 Review.)

5 Star
100%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used iCRT 14?

Submit a review and receive an Amazon gift card.

$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Useful Wnt inhibitor.
By Anonymous on 07/11/2020
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: HCT116

HCT116 cells were treated with different concentrations of iCRT-14 for 24 hours and the cells were used for transwell assays to investigate the effects of Wnt inhibitor on cell migration.

review image

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.


Cancer

Cancer Research Product Guide

A collection of over 750 products for cancer research, the guide includes research tools for the study of:

  • Cancer Metabolism
  • Epigenetics in Cancer
  • Receptor Signaling
  • Cell Cycle and DNA Damage Repair
  • Angiogenesis
  • Invasion and Metastasis

Pathways for iCRT 14

Wnt

Wnt Signaling Pathway

The Wnt pathway is involved in cellular differentiation and proliferation in adult tissues and also during embryogenesis. Disturbances within the pathway may lead to the formation of tumors and promote metastasis.