Hydroxystilbamidine bis(methanesulfonate)

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Cat.No. 6406 - Hydroxystilbamidine bis(methanesulfonate) | C16H16N4O.2CH4O3S | CAS No. 223769-64-0
Description: Displays different fluorescence emission wavelengths when bound to DNA compared to RNA
Chemical Name: 4-[2-[4-(Aminoiminomethyl)phenyl]ethenyl]-3-hydroxybenzenecarboximidamide bis(methanesulfonate)
Purity: ≥95% (HPLC)
Literature (1)

Biological Activity

Hydroxystilbamidine bis(methanesulfonate) is a fluorescent cationic dye commonly used as a neuronal tracer; exhibits retrograde axonal transport. Displays different fluorescence emission wavelengths when bound to DNA compared to RNA. Visualize with an ultraviolet excitation filter. Gold color is emitted when tissue is processed with a neutral pH buffer while blue/bright white is emitted when an acidic pH buffer is used.

Technical Data

M. Wt 472.54
Formula C16H16N4O.2CH4O3S
Storage Store at -20°C
Purity ≥95% (HPLC)
CAS Number 223769-64-0
PubChem ID 16212515
Smiles NC(C(C=C1)=CC=C1/C=C/C2=C(O)C=C(C(N)=N)C=C2)=N.CS(=O)(O)=O.CS(=O)(O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 23.63 50

Preparing Stock Solutions

The following data is based on the product molecular weight 472.54. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.5 mM 4.23 mL 21.16 mL 42.32 mL
2.5 mM 0.85 mL 4.23 mL 8.46 mL
5 mM 0.42 mL 2.12 mL 4.23 mL
25 mM 0.08 mL 0.42 mL 0.85 mL

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References are publications that support the biological activity of the product.

Chen et al (2012) Motor fiber organization in the extratemporal trunk of the facial nerve in rats: A retrograde Fluoro-Gold study. Exp.Ther.Med. 4 844 PMID: 23226737

Murgatroyd et al (1982) 2-hydroxystilbamidine isethionate: a new fluorochrome for use in general pathology. I. The selective staining of DNA, mucosubstances and elastic fibres. Histochemistry 74 107 PMID: 6177666

Buys et al (1995) (-)-Deprenyl increases the survival of rat retinal ganglion cells after optic nerve crush. Curr Eye Res. 14 119 PMID: 7768105

Day et al (2001) A beta-fiber intensity stimulation of chronically constricted median nerve induces c-fos expression in thalamic projection neurons of the cuneate nucleus in rats with behavioral signs of neuropathic pain. Brain Res. 895 194 PMID: 11259778

If you know of a relevant reference for Hydroxystilbamidine bis(methanesulfonate), please let us know.

Keywords: Hydroxystilbamidine bis(methanesulfonate), Hydroxystilbamidine bis(methanesulfonate) supplier, fluorescent, neuronal, dyes, retrograde, axonal, tracers, Fluoro, Gold, DNA, RNA, probe, Neuron, and, Astrocyte, Probes, Fluorescent, Stains, 6406, Tocris Bioscience

Citations for Hydroxystilbamidine bis(methanesulfonate)

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Literature in this Area

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Fluorescent Probes and Dyes Research Product Guide

Fluorescent Probes and Dyes Research Product Guide

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