Pricing Availability   Qty
Description: Potent and high affinity Smo receptor agonist
Chemical Name: 3-Chloro-4,7-difluoro-N-[trans-4-(methylamino)cyclohexyl]-N-[[3-(4-pyridinyl)phenyl]methyl]benzo[b]thiophene-2-carboxamide
Purity: ≥98% (HPLC)
Literature (1)

Biological Activity for Hh-Ag1.5

Hh-Ag1.5 is a potent and high affinity Smoothened (Smo) receptor agonist (EC50 = 1 nM; Ki values are 0.5 and 2.3 nM respectively, in a [3H]SAG-1.3 and [3H]Cyclopamine binding assay). It is used in protocols for the chemical reprogramming of mouse embryonic fibroblasts into neural stem cells. Also induces differentiation of hiPSCs into skin-derived precursor cells, spinal motor neurons and spinal sensory neurons.

Technical Data for Hh-Ag1.5

M. Wt 526.04
Formula C28H26ClF2N3OS
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 612542-14-0
PubChem ID 44195701
Smiles O=C(N(CC1=CC(C2=CC=NC=C2)=CC=C1)[C@H]3CC[C@H](NC)CC3)C(SC4=C(C=CC(F)=C54)F)=C5Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Hh-Ag1.5

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 52.6 100
ethanol 26.3 50

Preparing Stock Solutions for Hh-Ag1.5

The following data is based on the product molecular weight 526.04. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.9 mL 9.5 mL 19.01 mL
5 mM 0.38 mL 1.9 mL 3.8 mL
10 mM 0.19 mL 0.95 mL 1.9 mL
50 mM 0.04 mL 0.19 mL 0.38 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Dilution Calculator

Calculate the dilution required to prepare a stock solution.

References for Hh-Ag1.5

References are publications that support the biological activity of the product.

Frank-Kamenetsky et al (2002) Small-molecule modulators of Hedgehog signaling: identification and characterization of Smoothened agonists and antagonists. J.Biol. 1 10 PMID: 12437772

Rominger et al (2009) Evidence for allosteric interactions of antagonist binding to the smoothened receptor. J.Pharmacol.Exp.Ther. 329 995 PMID: 19304771

Takeda et al (2018) Chemical compound-based direct reprogramming for future clinical applications. Biosci.Rep. 38 BSR20171650 PMID: 29739872

Sugiyama-Nakagiri et al (2016) Induction of skin-derived precursor cells from human induced pluripotent stem cells. PLoS One 11 e0168451 PMID: 27992514

If you know of a relevant reference for Hh-Ag1.5, please let us know.

View Related Products by Product Action

View all Smoothened Receptor Agonists

Keywords: Hh-Ag1.5, Hh-Ag1.5 supplier, Potent, Smoothened, Smo, receptors, agonists, activates, Hedgehog, signaling, pathway, activator, enhancers, neuronal, differentiation, hiPSCs, induced, pluripotent, stem, cells, neurons, neurones, reprogramming, high, affinity, agonism, Receptors, Neural, Stem, Cells, Cell, Reprogramming, Other, Differentiation, Products, Signaling, 7807, Tocris Bioscience

Citations for Hh-Ag1.5

Citations are publications that use Tocris products.

Currently there are no citations for Hh-Ag1.5. Do you know of a great paper that uses Hh-Ag1.5 from Tocris? Please let us know.

Reviews for Hh-Ag1.5

There are currently no reviews for this product. Be the first to review Hh-Ag1.5 and earn rewards!

Have you used Hh-Ag1.5?

Submit a review and receive an Amazon gift card.

$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

Stem Cell Research Product Guide

Stem Cell Research Product Guide

This product guide provides a background to the use of small molecules in stem cell research and lists over 200 products for use in:

  • Self-renewal and Maintenance
  • Differentiation
  • Reprogramming
  • Organoid Generation
  • GMP and Ancillary Material Grade Products