HEPES Sodium salt

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Cat.No. 3174 - HEPES sodium salt | C8H17N2NaO4S | CAS No. 75277-39-3
Description: Multipurpose buffer
Chemical Name: 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt
Purity: ≥99.5%
Reviews (1)

Biological Activity

Multi purpose buffer used in biological research. Working pH range in aqueous solution: 6.8 - 8.2. Does not form complexes with metal ions. Used in cell culture media.

Technical Data

M. Wt 260.29
Formula C8H17N2NaO4S
Storage Store at RT
Purity ≥99.5%
CAS Number 75277-39-3
PubChem ID 2724248
Smiles [Na+].OCCN1CCN(CCS([O-])(=O)=O)CC1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Product Datasheets

Certificate of Analysis / Product Datasheet
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References are publications that support the biological activity of the product.

Good et al (1966) Hydrogen ion buffers for biological research. Biochemistry 5 467 PMID: 5942950

Medzon and Gedies (1971) Substitution of 4-(2-hydroxyethyl)-1-piperazineethane sulfonic acid (HEPES) for bicarbonate in protein-free animal cell culture medium: application to vaccinia virus quantitation and fluorogenic acetylesterase assay in living LM cells. Canadian J.Microbiol. 17 651

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Citations for HEPES Sodium salt

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HEPES Sodium salt used to make buffer for HEK 293 cell culture: Gives good buffer pH.
By Anonymous on 02/04/2019
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: HEK293

HEPES Sodium salt used to make buffer for HEK 293 cell culture. DMEM (Dulbecco’s Minimal Essential Media) with 10 % foetal calf serum (FCS) used as media.

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