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Submit ReviewG418 disulfate salt
Description: Aminoglycoside antibiotic; used in cell culture
Biological Activity for G418 disulfate salt
G418 disulfate salt is an aminoglycosidic antibiotic. Inhibits protein synthesis in both prokaryotic and eukaryotic cells. Used for selection of eukaryotic cells transfected with the neo gene.
This product has been cell culture tested.
Technical Data for G418 disulfate salt
| M. Wt | 692.71 |
| Formula | C20H40N4O10.2H2SO4 |
| Storage | Store at +4°C |
| CAS Number | 108321-42-2 |
| PubChem ID | 206347 |
| InChI Key | UHEPSJJJMTWUCP-DHDYTCSHSA-N |
| Smiles | N[C@H]1[C@H](O[C@]2([H])[C@H](O)[C@@H](NC)[C@@](C)(O)CO2)[C@@H](O)[C@H](O[C@]3([H])[C@H](N)[C@@H](O)[C@H](O)[C@]([C@H](O)C)([H])O3)[C@@H](N)C1.OS(O)(=O)=O.OS(O)(=O)=O |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for G418 disulfate salt
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| water | 51.95 | 75 |
Preparing Stock Solutions for G418 disulfate salt
The following data is based on the product molecular weight 692.71. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.75 mM | 1.92 mL | 9.62 mL | 19.25 mL |
| 3.75 mM | 0.38 mL | 1.92 mL | 3.85 mL |
| 7.5 mM | 0.19 mL | 0.96 mL | 1.92 mL |
| 37.5 mM | 0.04 mL | 0.19 mL | 0.38 mL |
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Product Datasheets for G418 disulfate salt
References for G418 disulfate salt
References are publications that support the biological activity of the product.
Wagman et al (1974) Antibiotic G-418, a new Micromonospora-produced aminoglycoside with activity against protozoa and helminths: fermentation, isolation and preliminary characterization. Antimicrob.Agents Chemother. 6 144 PMID: 15828184
Bar-Nun et al (1983) G-418, an elongation inhibitor of 80 S ribosomes. Biochim.Biophys.Acta. 741 123 PMID: 6193810
Burke and Mogg (1985) Suppression of a nonsense mutation in mammalian cells in vivo by the aminoglycoside antibiotics G-418 and paromo. Nucleic Acids Res. 13 6265 PMID: 2995924
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Keywords: G418 disulfate salt, G418 disulfate salt supplier, antibiotics, neomycin, resistance, cell, culture, aminoglycoside, inhibitors, inhibits, protein, synthesis, eukaryotes, eukaryotic, prokaryotes, prokaryotic, DNA,, RNA, and, Protein, Synthesis, Antibiotics, 4131, Tocris Bioscience
Citations for G418 disulfate salt
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Reviews for G418 disulfate salt
Average Rating: 4 (Based on 1 Review.)
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G418 maintains stable expression of recombinant hRyR2 in HEK293 cells..
By
Niroj Shrestha on 01/26/2019
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: HEK293
G418 was reconstituted in milli-Q water to get a stock solution of 50 mg/ml. The stock was diluted to 250 mcg/ml in DMEM containing 10% FBS, 4 mM glutamine, 10 mM HEPES and puromycin (5 mcg/ml) and used to culture HEK293 cell line, stably expressing hRyR2-IRES-GFP.
Stock solutions should be stored in aliquots at -20°C.
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