G418 disulfate salt
Biological Activity
Aminoglycosidic antibiotic. Inhibits protein synthesis in both prokaryotic and eukaryotic cells. Used for selection of eukaryotic cells transfected with the neo gene.
Technical Data
| M. Wt | 692.71 |
| Formula | C20H40N4O10.2H2SO4 |
| Storage | Store at +4°C |
| CAS Number | 108321-42-2 |
| PubChem ID | 206347 |
| InChI Key | UHEPSJJJMTWUCP-DHDYTCSHSA-N |
| Smiles | N[C@H]1[C@H](O[C@]2([H])[C@H](O)[C@@H](NC)[C@@](C)(O)CO2)[C@@H](O)[C@H](O[C@]3([H])[C@H](N)[C@@H](O)[C@H](O)[C@]([C@H](O)C)([H])O3)[C@@H](N)C1.OS(O)(=O)=O.OS(O)(=O)=O |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Solubility Data
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| water | 51.95 | 75 |
Preparing Stock Solutions
The following data is based on the product molecular weight 692.71. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.44 mL | 7.22 mL | 14.44 mL |
| 5 mM | 0.29 mL | 1.44 mL | 2.89 mL |
| 10 mM | 0.14 mL | 0.72 mL | 1.44 mL |
| 50 mM | 0.03 mL | 0.14 mL | 0.29 mL |
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References
References are publications that support the products' biological activity.
Wagman et al (1974) Antibiotic G-418, a new Micromonospora-produced aminoglycoside with activity against protozoa and helminths: fermentation, isolation and preliminary characterization. Antimicrob.Agents Chemother. 6 144 PMID: 15828184
Bar-Nun et al (1983) G-418, an elongation inhibitor of 80 S ribosomes. Biochim.Biophys.Acta. 741 123 PMID: 6193810
Burke and Mogg (1985) Suppression of a nonsense mutation in mammalian cells in vivo by the aminoglycoside antibiotics G-418 and paromomycin. Nucleic Acids Res. 13 6265 PMID: 2995924
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Keywords: antibiotics neomycin resistance cell culture aminoglycoside inhibitors inhibits protein synthesis eukaryotes eukaryotic prokaryotes prokaryotic DNA, RNA and Protein Synthesis
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