Submit a Review & Earn an Amazon Gift Card
You can now submit reviews for your favorite Tocris products. Your review will help other researchers decide on the best products for their research. Why not submit a review today?!
Submit ReviewG418 disulfate salt
Description: Aminoglycoside antibiotic; used in cell culture
Biological Activity for G418 disulfate salt
G418 disulfate salt is an aminoglycosidic antibiotic. Inhibits protein synthesis in both prokaryotic and eukaryotic cells. Used for selection of eukaryotic cells transfected with the neo gene.
This product has been cell culture tested.
Technical Data for G418 disulfate salt
| M. Wt | 692.71 |
| Formula | C20H40N4O10.2H2SO4 |
| Storage | Store at +4°C |
| CAS Number | 108321-42-2 |
| PubChem ID | 206347 |
| InChI Key | UHEPSJJJMTWUCP-DHDYTCSHSA-N |
| Smiles | N[C@H]1[C@H](O[C@]2([H])[C@H](O)[C@@H](NC)[C@@](C)(O)CO2)[C@@H](O)[C@H](O[C@]3([H])[C@H](N)[C@@H](O)[C@H](O)[C@]([C@H](O)C)([H])O3)[C@@H](N)C1.OS(O)(=O)=O.OS(O)(=O)=O |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Product Datasheets for G418 disulfate salt
References for G418 disulfate salt
References are publications that support the biological activity of the product.
Wagman et al (1974) Antibiotic G-418, a new Micromonospora-produced aminoglycoside with activity against protozoa and helminths: fermentation, isolation and preliminary characterization. Antimicrob.Agents Chemother. 6 144 PMID: 15828184
Bar-Nun et al (1983) G-418, an elongation inhibitor of 80 S ribosomes. Biochim.Biophys.Acta. 741 123 PMID: 6193810
Burke and Mogg (1985) Suppression of a nonsense mutation in mammalian cells in vivo by the aminoglycoside antibiotics G-418 and paromo. Nucleic Acids Res. 13 6265 PMID: 2995924
View Related Products by Target
View Related Products by Product Action
Keywords: G418 disulfate salt, G418 disulfate salt supplier, antibiotics, neomycin, resistance, cell, culture, aminoglycoside, inhibitors, inhibits, protein, synthesis, eukaryotes, eukaryotic, prokaryotes, prokaryotic, DNA,, RNA, and, Protein, Synthesis, Antibiotics, 4131, Tocris Bioscience
1 Citation for G418 disulfate salt
Citations are publications that use Tocris products. Selected citations for G418 disulfate salt include:
Yuan-Soon et al (2021) Curcumin-induced antitumor effects on triple-negative breast cancer patient-derived xenograft tumor mice through inhibiting salt-induced kinase-3 protein. J Food Drug Anal 29 622-637 PMID: 35649138
Reviews for G418 disulfate salt
Average Rating: 4 (Based on 1 Review.)
Have you used G418 disulfate salt?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Filter by:
G418 maintains stable expression of recombinant hRyR2 in HEK293 cells..
By
Niroj Shrestha on 01/26/2019
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: HEK293
G418 was reconstituted in milli-Q water to get a stock solution of 50 mg/ml. The stock was diluted to 250 mcg/ml in DMEM containing 10% FBS, 4 mM glutamine, 10 mM HEPES and puromycin (5 mcg/ml) and used to culture HEK293 cell line, stably expressing hRyR2-IRES-GFP.
Stock solutions should be stored in aliquots at -20°C.
Literature in this Area
Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!
*Please note that Tocris will only send literature to established scientific business / institute addresses.
Cell Cycle and DNA Damage Research Product Guide
This product guide provides a review of the cell cycle and DNA damage research area and lists over 150 products, including research tools for:
- Cell Cycle and Mitosis
- DNA Damage Repair
- Targeted Protein Degradation
- Ubiquitin Proteasome Pathway
- Chemotherapy Targets
Huntington's Disease Research Product Guide
This product guide provides a background to Huntington's disease research and lists around 100 products for the study of:
- Somatic Instability
- Proteolysis and Inclusion Bodies
- Transcriptional Dysregulation
- Mitochondrial Dysfunction
- Nuclear-Cytoplasmic Transport Interference
- Excitotoxicity
- Stem Cells