FFN 200 dihydrochloride

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Cat.No. 5911 - FFN 200 dihydrochloride | C11H14Cl2N2O2
Description: Selective fluorescent VMAT2 substrate
Chemical Name: 4-(2-Aminoethyl)-7-amino-2H-1-benzopyran-2-one dihydrochloride
Purity: ≥98% (HPLC)
Datasheet
Citations
Literature

Biological Activity

Selective fluorescent VMAT2 substrate. Selectively traces exocytosis in dopaminergic neuronal cell culture and brain tissue. Excitation and emission maxima are 352 and 451 nm, respectively.

Licensing Information

Sold with the permission of Columbia University.

FFN200 in Cultured Dopamine Neurons

FFN200 in vesicle clusters in axons of cultured dopaminergic neurons, and exocytosis upon high potassium stimulation (time lapse acquisition at 12 images/min).

Technical Data

M. Wt 277.15
Formula C11H14Cl2N2O2
Storage Store at -20°C
Purity ≥98% (HPLC)
PubChem ID 124201850
InChI Key YNQXRSNAOWBGLD-UHFFFAOYSA-N
Smiles NC1=CC(OC(C=C2CCN)=O)=C2C=C1.Cl.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 27.71 100
water 27.71 100

Preparing Stock Solutions

The following data is based on the product molecular weight 277.15. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.61 mL 18.04 mL 36.08 mL
5 mM 0.72 mL 3.61 mL 7.22 mL
10 mM 0.36 mL 1.8 mL 3.61 mL
50 mM 0.07 mL 0.36 mL 0.72 mL

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Product Datasheets

Safety Datasheet

References

References are publications that support the products' biological activity.

Pereira et al (2015) Fluorescent false neurotransmitter reveals functionally silent dopamine vesicle clusters in the striatum. Nat.Neurosci. 19 578 PMID: 26900925

Li (2017) Probes for monitoring regulated exocytosis Cell Calcium PMID:


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Keywords: FFN200 dihydrochloride fluorescent false neurotransmitters VMAT2 vesicular monoamine transporters 2 dopaminergic substrates Fluorescent Probes

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New Product Guide

New Product Guide (Spring/Summer 2017)

Our new product guide highlights over 130 new products added to the Tocris Bioscience range during the first half of 2017.

  • 7-TM Receptors
  • Enzymes
  • Enzyme-Linked Receptors
  • Ion Channels
  • Nuclear Receptors
  • Transporters
  • Apoptosis
  • Cell Metabolism
  • Epigenetics
  • Fluorescent Imaging
  • Signal Transduction
  • Stem Cells

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