FFN 200 dihydrochloride

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Description: Selective fluorescent VMAT2 substrate
Chemical Name: 4-(2-Aminoethyl)-7-amino-2H-1-benzopyran-2-one dihydrochloride
Purity: ≥98% (HPLC)
Citations (1)
Literature (1)

Biological Activity for FFN 200 dihydrochloride

FFN 200 dihydrochloride is a selective fluorescent VMAT2 substrate. Selectively traces exocytosis in dopaminergic neuronal cell culture and brain tissue. Excitation and emission maxima are 352 and 451 nm, respectively.

Licensing Information

Sold with the permission of Columbia University.

Applications in Cultured Dopamine Neurons for FFN 200 dihydrochloride

FFN200 in vesicle clusters in axons of cultured dopaminergic neurons, and exocytosis upon high potassium stimulation (time lapse acquisition at 12 images/min).

Technical Data for FFN 200 dihydrochloride

M. Wt 277.15
Formula C11H12N2O2.2HCl
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 2080306-27-8
PubChem ID 124201850
Smiles NC1=CC(OC(C=C2CCN)=O)=C2C=C1.Cl.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for FFN 200 dihydrochloride

Solvent Max Conc. mg/mL Max Conc. mM
water 27.71 100
DMSO 27.71 100

Preparing Stock Solutions for FFN 200 dihydrochloride

The following data is based on the product molecular weight 277.15. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.61 mL 18.04 mL 36.08 mL
5 mM 0.72 mL 3.61 mL 7.22 mL
10 mM 0.36 mL 1.8 mL 3.61 mL
50 mM 0.07 mL 0.36 mL 0.72 mL

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Product Datasheets for FFN 200 dihydrochloride

References for FFN 200 dihydrochloride

References are publications that support the biological activity of the product.

Pereira et al (2015) Fluorescent false neurotransmitter reveals functionally silent DA vesicle clusters in the striatum. Nat.Neurosci. 19 578 PMID: 26900925

Li (2017) Probes for monitoring regulated exocytosis. Cell Calcium 64 65 PMID: 28089267

If you know of a relevant reference for FFN 200 dihydrochloride, please let us know.

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1 Citation for FFN 200 dihydrochloride

Citations are publications that use Tocris products. Selected citations for FFN 200 dihydrochloride include:

Matthias et al (2022) Endogenously produced catecholamines improve the regulatory function of TLR9-activated B cells. PLoS Biol 20 e3001513 PMID: 35073310

Do you know of a great paper that uses FFN 200 dihydrochloride from Tocris? Please let us know.

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Literature in this Area

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Fluorescent Dyes and Probes Research Product Guide

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