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FFN 200 dihydrochloride
Selective fluorescent VMAT2 substrate. Selectively traces exocytosis in dopaminergic neuronal cell culture and brain tissue. Excitation and emission maxima are 352 and 451 nm, respectively.
Sold with the permission of Columbia University.
FFN200 in Cultured Dopamine Neurons
FFN200 in vesicle clusters in axons of cultured dopaminergic neurons, and exocytosis upon high potassium stimulation (time lapse acquisition at 12 images/min).
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions
The following data is based on the product molecular weight 277.15. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||3.61 mL||18.04 mL||36.08 mL|
|5 mM||0.72 mL||3.61 mL||7.22 mL|
|10 mM||0.36 mL||1.8 mL||3.61 mL|
|50 mM||0.07 mL||0.36 mL||0.72 mL|
References are publications that support the biological activity of the product.
Pereira et al (2015) Fluorescent false neurotransmitter reveals functionally silent DA vesicle clusters in the striatum. Nat.Neurosci. 19 578 PMID: 26900925
Li (2017) Probes for monitoring regulated exocytosis. Cell Calcium 64 65 PMID: 28089267
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