FFN 200 dihydrochloride
Selective fluorescent VMAT2 substrate. Selectively traces exocytosis in dopaminergic neuronal cell culture and brain tissue. Excitation and emission maxima are 352 and 451 nm, respectively.
Sold with the permission of Columbia University.
FFN200 in Cultured Dopamine Neurons
FFN200 in vesicle clusters in axons of cultured dopaminergic neurons, and exocytosis upon high potassium stimulation (time lapse acquisition at 12 images/min).
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions
The following data is based on the product molecular weight 277.15. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||3.61 mL||18.04 mL||36.08 mL|
|5 mM||0.72 mL||3.61 mL||7.22 mL|
|10 mM||0.36 mL||1.8 mL||3.61 mL|
|50 mM||0.07 mL||0.36 mL||0.72 mL|
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.
References are publications that support the products' biological activity.
Pereira et al (2015) Fluorescent false neurotransmitter reveals functionally silent dopamine vesicle clusters in the striatum. Nat.Neurosci. 19 578 PMID: 26900925
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Keywords: FFN200 dihydrochloride fluorescent false neurotransmitters VMAT2 vesicular monoamine transporters 2 dopaminergic substrates Fluorescent Probes
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