Eact

Pricing Availability Delivery Time Qty
Cat.No. 4876 - Eact | C22H24N2O5S | CAS No. 461000-66-8
Description: Activator of Ca2+-activated Cl- channel transmembrane protein 16A (TMEM16A)
Chemical Name: 3,4,5-Trimethoxy-N-(2-methoxyethyl)-N-(4-phenyl-2-thiazolyl)benzamide
Purity: ≥99% (HPLC)
Datasheet
Citations
Literature
Pathways

Biological Activity

TMEM16A (ANO1) calcium-activated chloride channel (CaCC) activator (EC50 = 3 μM). Also transient receptor potential vanilloid 1 (TRPV1) channel activator (EC50 = 11.6 μM). Ca2+-independent. Displays no effect on CFTR Cl- or ENaC Na+ conductance. Stimulates submucosal gland secretion in human bronchi.

Compound Libraries

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Technical Data

M. Wt 428.5
Formula C22H24N2O5S
Storage Store at +4°C
Purity ≥99% (HPLC)
CAS Number 461000-66-8
PubChem ID 3173542
InChI Key ZUXNHFFVQWADJL-UHFFFAOYSA-N
Smiles O=C(C3=CC(OC)=C(OC)C(OC)=C3)N(CCOC)C1=NC(C2=CC=CC=C2)=CS1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 42.85 100
ethanol 4.29 10

Preparing Stock Solutions

The following data is based on the product molecular weight 428.5. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.33 mL 11.67 mL 23.34 mL
5 mM 0.47 mL 2.33 mL 4.67 mL
10 mM 0.23 mL 1.17 mL 2.33 mL
50 mM 0.05 mL 0.23 mL 0.47 mL

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Product Datasheets

Safety Datasheet

References

References are publications that support the products' biological activity.

Namkung et al (2011) Small-molecule activators of TMEM16A, a calcium-activated chloride channel, stimulate epithelial chloride secretion and intestinal contraction. FASEB J. 25 4048 PMID: 21836025

Liu et al (2016) Eact, a small molecule activator of TMEM16A, activates TRPV1 and elicits pain- and itch-related behaviours. Br.J.Pharmacol. 173 1208 PMID: 26756551


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Citations for Eact

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Literature in this Area

Cardiovascular

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  • Hypertension
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Programmed Cell Death

Programmed Cell Death Poster

There are two currently recognized forms of programmed cell death: apoptosis and necroptosis. This poster summarizes the signaling pathways involved in apoptosis, necroptosis and cell survival following death receptor activation, and highlights the influence of the molecular switch, cFLIP, on cell fate.

Pathways for Eact

Apoptosis

Apoptosis Signaling Pathway

Apoptosis is a physiological process for cell death that is critical during aging and development. It may also be referred to as cell 'suicide'. Apoptosis can be triggered by events both inside and outside of the cell.

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