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Eact

Cat. No. 4876 Submit a Review Datasheet / COA / SDS
Pricing Availability   Qty
Description: Activator of Ca2+-activated Cl- channel transmembrane protein 16A (TMEM16A)
Chemical Name: 3,4,5-Trimethoxy-N-(2-methoxyethyl)-N-(4-phenyl-2-thiazolyl)benzamide
Purity: ≥98% (HPLC)
Datasheet
Citations
Reviews
Literature (4)
Pathways (1)
Biological Activity Technical Data Solubility Calculators Datasheets References

Biological Activity for Eact

Eact is a TMEM16A (ANO1) calcium-activated chloride channel (CaCC) activator (EC50 = 3 μM). Also transient receptor potential vanilloid 1 (TRPV1) channel activator (EC50 = 11.6 μM). Ca2+-independent. Displays no effect on CFTR Cl- or ENaC Na+ conductance. Stimulates submucosal gland secretion in human bronchi.

Compound Libraries for Eact

Eact is also offered as part of the Tocriscreen 2.0 Max. Find out more about compound libraries available from Tocris.

Technical Data for Eact

M. Wt 428.5
Formula C22H24N2O5S
Storage Store at +4°C
Purity ≥98% (HPLC)
CAS Number 461000-66-8
PubChem ID 3173542
InChI Key ZUXNHFFVQWADJL-UHFFFAOYSA-N
Smiles O=C(C3=CC(OC)=C(OC)C(OC)=C3)N(CCOC)C1=NC(C2=CC=CC=C2)=CS1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Eact

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 42.85 100

Preparing Stock Solutions for Eact

The following data is based on the product molecular weight 428.5. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.33 mL 11.67 mL 23.34 mL
5 mM 0.47 mL 2.33 mL 4.67 mL
10 mM 0.23 mL 1.17 mL 2.33 mL
50 mM 0.05 mL 0.23 mL 0.47 mL

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References for Eact

References are publications that support the biological activity of the product.

Namkung et al (2011) Small-molecule activators of TMEM16A, a calcium-activated chloride channel, stimulate epithelial chloride secretion and intestinal contraction. FASEB J. 25 4048 PMID: 21836025

Liu et al (2016) Eact, a small molecule activator of TMEM16A, activates TRPV1 and elicits pain- and itch-related behaviours. Br.J.Pharmacol. 173 1208 PMID: 26756551

If you know of a relevant reference for Eact, please let us know.

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Keywords: Eact, Eact supplier, TMEM16A, ANO1, calcium-activated, chloride, channels, CaCC, activators, activates, anoctamin-1, submucosal, secretion, Ca2+-Activated, Chloride, Channels, TRPV, 4876, Tocris Bioscience

Citations for Eact

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