E 64
Biological Activity
Potent and irreversible cysteine protease inhibitor (IC50 values are 1.4, 2.5 and 4.1 nM for cathepsin K, L and S respectively. Also inhibits papain, calpain, cathepsin B and cathepsin H. Has no effect on serine proteases. Reduces HSC-3 tongue cancer cell invasion and induces oxidative stress and apoptosis in filarial parasites. Also inhibits autophagy-associated lysosomal degradation in vitro.
Technical Data
| M. Wt | 357.41 |
| Formula | C15H27N5O5 |
| Storage | Store at -20°C |
| Purity | ≥98% (HPLC) |
| CAS Number | 66701-25-5 |
| PubChem ID | 123985 |
| InChI Key | LTLYEAJONXGNFG-DCAQKATOSA-N |
| Smiles | O=[C@@](N[C@H]([C@](NCCCCNC(N)=N)=O)CC(C)C)[C@@H]1[C@@H](C(O)=O)O1 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Solubility Data
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| water | 7.15 | 20 |
Preparing Stock Solutions
The following data is based on the product molecular weight 357.41. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.2 mM | 13.99 mL | 69.95 mL | 139.9 mL |
| 1 mM | 2.8 mL | 13.99 mL | 27.98 mL |
| 2 mM | 1.4 mL | 6.99 mL | 13.99 mL |
| 10 mM | 0.28 mL | 1.4 mL | 2.8 mL |
Molarity Calculator
Reconstitution Calculator
Dilution Calculator
Product Datasheets
References
References are publications that support the biological activity of the product.
Susa et al (2004) Human primary osteoclasts: in vitro generation and applications as pharmacological and clinical assay. J.Transl.Med. 2 6 PMID: 15025786
Bitu et al (2013) Cathepsin K is present in invasive oral tongue squamous cell carcinoma in vivo and in vitro. PLoS ONE 8 e70925 PMID: 23951042
Wadhawan et al (2014) Inhibition of cathepsin B by E-64 induces oxidative stress and apoptosis in filarial parasite. PLoS ONE 9 e93161 PMID: 24667798
Zhao et al (2010) Cytosolic FoxO1 is essential for the induction of autophagy and tumour suppressor activity. Nat.Cell.Biol. 12 665 PMID: 20543840
If you know of a relevant reference for E 64, please let us know.
View Related Products by Target
View Related Products by Product Action
Keywords: E 64, E 64 supplier, E64, potent, irreversible, natural, cysteine, protease, inhibitors, inhibits, cathepsin, K, L, S, B, H, calpain, papain, Cathepsin, Calpains, Calcium-Sensitive, Proteases, 5208, Tocris Bioscience
1 Citation for E 64
Citations are publications that use Tocris products. Selected citations for E 64 include:
Lee et al (2015) Tricyclic Antidepressants Amitriptyline and Desipramine Induced Neurotoxicity Associated with Parkinson's Disease. PLoS One 38 734 PMID: 26242194
Do you know of a great paper that uses E 64 from Tocris? Please let us know.
Reviews for E 64
There are currently no reviews for this product. Be the first to review E 64 and earn rewards!
Have you used E 64?
Submit a review and receive an Amazon gift card.
$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Literature in this Area
Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* or download your copy today!
*Please note that Tocris will only send literature to established scientific business / institute addresses.
New Product Guide (Spring/Summer 2018)
Our new product guide highlights over 200 new products added to the Tocris Bioscience range during the second half of 2017.
- 7-TM Receptors
- Enzymes
- Enzyme-Linked Receptors
- Ion Channels
- Nuclear Receptors
- Transporters
- Aptamers
- Cell Metabolism
- Epigenetics
- Fluorescent Imaging
- Signal Transduction
- Stem Cells
