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DOPE is a liposome/lipoplex transfection reagent. Enhances liposome and lipoplex transfection efficiency in combination with DA, DOTAP or DC-cholesterol (Cat. No. 7176).
Please note: exact ratio for use and transfection efficiency is dependent on cell line used.
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
The following data is based on the product molecular weight 744.05. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||1.34 mL||6.72 mL||13.44 mL|
|5 mM||0.27 mL||1.34 mL||2.69 mL|
|10 mM||0.13 mL||0.67 mL||1.34 mL|
|50 mM||0.03 mL||0.13 mL||0.27 mL|
References are publications that support the biological activity of the product.
Mochizuki et al (2013) The role of the helper lipid dioleoylphosphatidylethanolamine (DOPE) for DNA transfection cooperating with a cationic lipid bearing ethylenediamine. Biochim.Biophys.Acta. 1828 412 PMID: 23092705
Kim et al (2015) DOTAP/DOPE ratio and cell type determine transfection efficiency with DOTAP-liposomes. Biochim.Biophys.Acta. 1848 1996 PMID: 26112463
If you know of a relevant reference for DOPE, please let us know.
Keywords: DOPE, DOPE supplier, non-viral, lipoplex, liposome, transfection, reagents, efficicency, enhancers, enhances, Biochemicals, and, Molecular, Biology, 7175, Tocris Bioscience
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