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Description: Nitric oxide spin trap; measure oxygen-centered free radicals
Chemical Name: 3,4-Dihydro-2,2-dimethyl-2H-pyrrole 1-oxide
Biological Activity
Water soluble nitric oxide spin trap; allows the measurement of oxygen-centered free radicals in biological systems at room temperature using electron spin resonance (ESR). Has a high reaction rate constant for superoxide and hydroxyl radicals, and distinguishes simultaneously among a variety of important biologically generated free radicals.
Technical Data
| M. Wt | 113.16 |
| Formula | C6H11NO |
| Storage | Store at -20°C |
| CAS Number | 3317-61-1 |
| PubChem ID | 1774 |
| InChI Key | VCUVETGKTILCLC-UHFFFAOYSA-N |
| Smiles | [O-][N+]1=CCCC(C)1C |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 11.32 | 100 | |
| ethanol | 11.32 | 100 |
Preparing Stock Solutions
The following data is based on the product molecular weight 113.16. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 8.84 mL | 44.19 mL | 88.37 mL |
| 5 mM | 1.77 mL | 8.84 mL | 17.67 mL |
| 10 mM | 0.88 mL | 4.42 mL | 8.84 mL |
| 50 mM | 0.18 mL | 0.88 mL | 1.77 mL |
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Product Datasheets
References
References are publications that support the biological activity of the product.
Togashi et al (2000) Analysis of hepatic oxidative stress status by electron spin resonance spectroscopy and imaging. Free Radic.Biol.Med. 28 846 PMID: 10802214
Nohara et al (2009) The generation of lucigenin chemiluminescence from the reaction of guanidino compounds with phenylglyoxal under alkaline conditions and its application. Chem.Pharm.Bull. 57 700 PMID: 19571414
Saito et al (2009) Comparison of superoxide detection abilities of newly developed spin traps in the living cells. Free Radic.Res. 43 668 PMID: 19479584
If you know of a relevant reference for DMPO, please let us know.
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Keywords: DMPO, DMPO supplier, Nitric, oxide, spin, trap, measure, oxygen-centered, free, radicals, Reagents, Signaling, Signalling, Other, Oxide, Biochemicals, and, Molecular, Biology, 3415, Tocris Bioscience
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