DMPO

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Cat.No. 3415 - DMPO | C6H11NO | CAS No. 3317-61-1
Description: Nitric oxide spin trap; measure oxygen-centered free radicals
Chemical Name: 3,4-Dihydro-2,2-dimethyl-2H-pyrrole 1-oxide
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Biological Activity

Water soluble nitric oxide spin trap; allows the measurement of oxygen-centered free radicals in biological systems at room temperature using electron spin resonance (ESR). Has a high reaction rate constant for superoxide and hydroxyl radicals, and distinguishes simultaneously among a variety of important biologically generated free radicals.

Technical Data

M. Wt 113.16
Formula C6H11NO
Storage Store at -20°C
CAS Number 3317-61-1
PubChem ID 1774
InChI Key VCUVETGKTILCLC-UHFFFAOYSA-N
Smiles [O-][N+]1=CCCC(C)1C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 11.32 100
ethanol 11.32 100

Preparing Stock Solutions

The following data is based on the product molecular weight 113.16. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 8.84 mL 44.19 mL 88.37 mL
5 mM 1.77 mL 8.84 mL 17.67 mL
10 mM 0.88 mL 4.42 mL 8.84 mL
50 mM 0.18 mL 0.88 mL 1.77 mL

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References

References are publications that support the biological activity of the product.

Togashi et al (2000) Analysis of hepatic oxidative stress status by electron spin resonance spectroscopy and imaging. Free Radic.Biol.Med. 28 846 PMID: 10802214

Nohara et al (2009) The generation of lucigenin chemiluminescence from the reaction of guanidino compounds with phenylglyoxal under alkaline conditions and its application. Chem.Pharm.Bull. 57 700 PMID: 19571414

Saito et al (2009) Comparison of superoxide detection abilities of newly developed spin traps in the living cells. Free Radic.Res. 43 668 PMID: 19479584


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Keywords: DMPO, DMPO supplier, Nitric, oxide, spin, trap, measure, oxygen-centered, free, radicals, Reagents, Signaling, Signalling, Other, Oxide, Biochemicals, and, Molecular, Biology, 3415, Tocris Bioscience

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