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Cat.No. 4502 - DIM | C17H14N2 | CAS No. 1968-05-4
Description: Inhibits EGFR activation; also induces apoptosis
Alternative Names: 3,3-Diindolylmethane
Chemical Name: 3,3'-Methylenediindole
Purity: ≥99% (HPLC)
Reviews (1)
Literature (4)

Biological Activity

Activator of Chk2 that causes G2/M cell cycle arrest in various cancer cell lines. Antiproliferative and inducer of apoptosis; promotes proteasomal degradation of Cdc25C and Cdk1. Inhibits phosphorylation of EGFR and downstream activation of ERK.

Technical Data

M. Wt 246.31
Formula C17H14N2
Storage Store at +4°C
Purity ≥99% (HPLC)
CAS Number 1968-05-4
PubChem ID 10900930
Smiles C12=CC=CC=C1C(CC3=CC(C=CC=C4)=C4N3)=CN2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 24.63 100
ethanol 12.32 50

Preparing Stock Solutions

The following data is based on the product molecular weight 246.31. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 4.06 mL 20.3 mL 40.6 mL
5 mM 0.81 mL 4.06 mL 8.12 mL
10 mM 0.41 mL 2.03 mL 4.06 mL
50 mM 0.08 mL 0.41 mL 0.81 mL

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References are publications that support the biological activity of the product.

Kandala et al (2012) Blocking epidermal growth factor receptor activation by 3,3'-diindolylmethane suppresses ovarian tumor growth in vitro and in vivo. J.Pharmacol.Exp.Ther. 341 24 PMID: 22205686

Kandala & Srivastava (2010) Activation of checkpoint kinase 2 by 3,3'-diindolylmethane is required for causing G2/M cell cycle arrest in human ovarian cancer cells. Mol.Pharmacol. 78 297 PMID: 20444961

Shorey et al (2012) 3,3'-diindolylmethane induces G1 arrest and apoptosis in human acute T-cell lymphoblastic leukemia cells. PLoS One. 7 e34975 PMID: 22514694

If you know of a relevant reference for DIM, please let us know.

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Citations for DIM

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Determination of DIM suppression of EGFR.
By Anonymous on 12/14/2019
Species: Human

To determine whether DIM suppressed the activation of EGFR by activating phosphorylation, cells were treated with epidermal growth factor.

review image

Literature in this Area

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Cancer Research Product Guide

A collection of over 750 products for cancer research, the guide includes research tools for the study of:

  • Cancer Metabolism
  • Epigenetics in Cancer
  • Receptor Signaling
  • Cell Cycle and DNA Damage Repair
  • Angiogenesis
  • Invasion and Metastasis

Kinases Product Listing

A collection of over 400 products for kinase research, the listing includes inhibitors of:

  • Receptor Tyrosine Kinases
  • Protein Kinases A, C, D and G
  • PI-3 Kinase, Akt and mTOR
  • MAPK Signaling
  • Receptor Serine/Threonine Kinases
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