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Cat.No. 6882 - DiFMUP | C10H7F2O6P | CAS No. 214491-43-7
Description: Fluorescent phosphatase substrate
Chemical Name: 6,8-Difluoro-4-methyl-7-(phosphonooxy)-2H-1-benzopyran-2-one
Purity: ≥95% (HPLC)
Literature (1)

Biological Activity

Fluorescent phophatase substrate; widely used for the detection of phosphatase activity. Excitation/emission maxima are 358/455 nm.

Technical Data

M. Wt 292.13
Formula C10H7F2O6P
Storage Store at -20°C
Purity ≥95% (HPLC)
CAS Number 214491-43-7
PubChem ID 2786976
Smiles CC1=CC(=O)OC2=C(F)C(O[P](O)(O)=O)=C(F)C=C12

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 29.21 100

Preparing Stock Solutions

The following data is based on the product molecular weight 292.13. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.42 mL 17.12 mL 34.23 mL
5 mM 0.68 mL 3.42 mL 6.85 mL
10 mM 0.34 mL 1.71 mL 3.42 mL
50 mM 0.07 mL 0.34 mL 0.68 mL

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References are publications that support the biological activity of the product.

Welte et al (2005) 6,8-Difluoro-4-methylumbiliferyl phosphate: a fluorogenic substrate for protein tyrosine phosphatases. Anal.Biochem. 338 32 PMID: 15707933

Gee et al (1999) Fluorogenic substrates based on fluorinated umbelliferones for continuous assays of phosphatases and beta-galactosidases. Anal.Biochem. 273 41 PMID: 10452797

If you know of a relevant reference for DiFMUP, please let us know.

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Citations for DiFMUP

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Literature in this Area

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