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Cat.No. 7324 - Di 4 ANEPPS | C28H36N2O3S | CAS No. 90134-00-2
Description: Voltage-sensitive probe; used to detect changes in membrane potential in electrophysiology protocols
Chemical Name: 6-[2-(N,N-Dibutylamino)naphthyl]ethenyl-4'-pyridinium propanesulfonate
Purity: ≥90% (HPLC)
Literature (1)

Biological Activity for Di 4 ANEPPS

Di 4 ANEPPS is a voltage-sensitive dye, also known as an electrochromic probe, which fluoresces in response to change in electrical potential across a cellular membrane. Di 4 ANEPPS fluoresces only when membrane bound; change in electrical potential causes an intramolecular charge transfer and blue-shift in the excitation spectra. Di 4 ANEPPS is commonly used to detect transient changes in membrane potential in excitable cells such as neurons and cardiac cells. Excitation ratio measurements can be used to determine membrane potential.

In model phospholipid membranes Ex/Em maxima are 465/635 nm, however spectral properties are highly dependent on membrane environment.

Optical Data for Di 4 ANEPPS

λabs 465 nm
λem 635 nm
Application Electrophysiology

Technical Data for Di 4 ANEPPS

M. Wt 480.67
Formula C28H36N2O3S
Storage Store at -20°C
Purity ≥90% (HPLC)
CAS Number 90134-00-2
PubChem ID 6438327
Smiles [O-]S(CCC[N+](C=C1)=CC=C1/C=C/C(C=C2C=C3)=CC=C2C=C3N(CCCC)CCCC)(=O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Di 4 ANEPPS

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 4.81 10

Preparing Stock Solutions for Di 4 ANEPPS

The following data is based on the product molecular weight 480.67. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.1 mM 20.8 mL 104.02 mL 208.04 mL
0.5 mM 4.16 mL 20.8 mL 41.61 mL
1 mM 2.08 mL 10.4 mL 20.8 mL
5 mM 0.42 mL 2.08 mL 4.16 mL

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References for Di 4 ANEPPS

References are publications that support the biological activity of the product.

Loew et al (1992) A naphthyl analog of the aminostyryl pyridinium class of potentiometric membrane dyes shows consistent sensitivity in a variety of tissue, cell, and model membrane preparations. J.Membr.Biol. 130 1 PMID: 1469705

Tominga & Tominga (2013) A new nonscanning confocal microscopy module for functional voltage-sensitive dye and Ca2+ imaging of neuronal circuit activity. J.Neurophysiol. 110 553 PMID: 23615547

Asamoah et al (2003) A fluorometric approach to local electric field measurements in a voltage-gated ion channel. Neuron. 37 85 PMID: 12526775

If you know of a relevant reference for Di 4 ANEPPS, please let us know.

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