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Di 4 ANEPPS New
Biological Activity for Di 4 ANEPPS
Di 4 ANEPPS is a voltage-sensitive dye, also known as an electrochromic probe, which fluoresces in response to change in electrical potential across a cellular membrane. Di 4 ANEPPS fluoresces only when membrane bound; change in electrical potential causes an intramolecular charge transfer and blue-shift in the excitation spectra. Di 4 ANEPPS is commonly used to detect transient changes in membrane potential in excitable cells such as neurons and cardiac cells. Excitation ratio measurements can be used to determine membrane potential.
In model phospholipid membranes Ex/Em maxima are 465/635 nm, however spectral properties are highly dependent on membrane environment.
Optical Data for Di 4 ANEPPS
Technical Data for Di 4 ANEPPS
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Di 4 ANEPPS
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions for Di 4 ANEPPS
The following data is based on the product molecular weight 480.67. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|0.1 mM||20.8 mL||104.02 mL||208.04 mL|
|0.5 mM||4.16 mL||20.8 mL||41.61 mL|
|1 mM||2.08 mL||10.4 mL||20.8 mL|
|5 mM||0.42 mL||2.08 mL||4.16 mL|
References for Di 4 ANEPPS
References are publications that support the biological activity of the product.
Loew et al (1992) A naphthyl analog of the aminostyryl pyridinium class of potentiometric membrane dyes shows consistent sensitivity in a variety of tissue, cell, and model membrane preparations. J.Membr.Biol. 130 1 PMID: 1469705
Tominga & Tominga (2013) A new nonscanning confocal microscopy module for functional voltage-sensitive dye and Ca2+ imaging of neuronal circuit activity. J.Neurophysiol. 110 553 PMID: 23615547
Asamoah et al (2003) A fluorometric approach to local electric field measurements in a voltage-gated ion channel. Neuron. 37 85 PMID: 12526775
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Citations for Di 4 ANEPPS
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