DFHBI 1T

Pricing Availability Delivery Time Qty
Cat.No. 5610 - DFHBI 1T | C13H9F5N2O2 | CAS No. 1539318-36-9
Description: Mimic of GFP fluorophore for imaging RNA in living cells
Chemical Name: (5Z)-5-[(3,5-Difluoro-4-hydroxyphenyl)methylene]-3,5-dihydro-2-methyl-3-(2,2,2-trifluoroethyl)-4H-imidazol-4-one
Purity: ≥98% (HPLC)
Datasheet
Citations
Literature

Biological Activity

Mimic of green fluorescent protein (GFP) fluorophore for imaging RNA in living cells. Fluorescence activated by binding to Spinach2 or Broccoli aptamers. Exhibits peak excitation maxima of 482 nm and peak fluorescence emission of 505 nm when bound to Spinach2, enabling imaging with GFP filter cubes. Exhibits higher specific fluorescence and lower background fluorescence when bound to Spinach2 compared with Spinach2-DFHBI.

Technical Data

M. Wt 320.21
Formula C13H9F5N2O2
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 1539318-36-9
PubChem ID 101889712
InChI Key AWYCLBWNRONMQC-WMZJFQQLSA-N
Smiles O=C1N(CC(F)(F)F)C(C)=N\C1=C/C2=CC(F)=C(O)C(F)=C2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 32.02 100
ethanol 16.01 50

Preparing Stock Solutions

The following data is based on the product molecular weight 320.21. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.12 mL 15.61 mL 31.23 mL
5 mM 0.62 mL 3.12 mL 6.25 mL
10 mM 0.31 mL 1.56 mL 3.12 mL
50 mM 0.06 mL 0.31 mL 0.62 mL

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Product Datasheets

Certificate of Analysis / Product Datasheet
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Safety Datasheet

References

References are publications that support the products' biological activity.

Song et al (2014) Plug-and-play fluorophores extend the spectral properties of Spinach. J.Am.Chem.Soc. 136 198 PMID: 24393009

Filonov et al (2015) In-gel imaging of RNA processing using broccoli reveals optimal aptamer expression strategies. Chem.Biol. 22 649 PMID: 26000751

Svensen and Jaffrey (2016) Fluorescent RNA Aptamers as a Tool to Study RNA-Modifying Enzymes. Cell Chem.Biol. 23 415 PMID: 26877022


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New Product Guide

New Product Guide (Spring/Summer 2017)

Our new product guide highlights over 130 new products added to the Tocris Bioscience range during the first half of 2017.

  • 7-TM Receptors
  • Enzymes
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  • Apoptosis
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  • Epigenetics
  • Fluorescent Imaging
  • Signal Transduction
  • Stem Cells

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