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Submit ReviewDFHBI 1T
Description: GFP fluorophore mimic for imaging RNA in living cells; activated by binding Spinach2 and Broccoli aptamers
Chemical Name: (5Z)-5-[(3,5-Difluoro-4-hydroxyphenyl)methylene]-3,5-dihydro-2-methyl-3-(2,2,2-trifluoroethyl)-4H-imidazol-4-one
Purity: ≥98% (HPLC)
Biological Activity for DFHBI 1T
DFHBI 1T is a mimic of green fluorescent protein (GFP) fluorophore for imaging RNA in living cells. DFHBI 1T fluorescence is activated by binding to Spinach2 or Broccoli aptamers, also binds to Squash aptamer (Kd = 45 nM). DFHBI 1T exhibits peak excitation maxima of 482 nm and peak fluorescence emission of 505 nm when bound to Spinach2, enabling imaging with GFP filter cubes. Exhibits higher specific fluorescence and lower background fluorescence when bound to Spinach2 compared with Spinach2-DFHBI.
Technical Data for DFHBI 1T
| M. Wt | 320.21 |
| Formula | C13H9F5N2O2 |
| Storage | Store at -20°C |
| Purity | ≥98% (HPLC) |
| CAS Number | 1539318-36-9 |
| PubChem ID | 101889712 |
| InChI Key | AWYCLBWNRONMQC-WMZJFQQLSA-N |
| Smiles | O=C1N(CC(F)(F)F)C(C)=N\C1=C/C2=CC(F)=C(O)C(F)=C2 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for DFHBI 1T
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 32.02 | 100 |
Preparing Stock Solutions for DFHBI 1T
The following data is based on the product molecular weight 320.21. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 3.12 mL | 15.61 mL | 31.23 mL |
| 5 mM | 0.62 mL | 3.12 mL | 6.25 mL |
| 10 mM | 0.31 mL | 1.56 mL | 3.12 mL |
| 50 mM | 0.06 mL | 0.31 mL | 0.62 mL |
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Product Datasheets for DFHBI 1T
References for DFHBI 1T
References are publications that support the biological activity of the product.
Song et al (2014) Plug-and-play fluorophores extend the spectral properties of Spinach. J.Am.Chem.Soc. 136 198 PMID: 24393009
Filonov et al (2015) In-gel imaging of RNA processing using broccoli reveals optimal aptamer expression strategies. Chem.Biol. 22 649 PMID: 26000751
Svensen and Jaffrey (2016) Fluorescent RNA Aptamers as a Tool to Study RNA-Modifying Enzymes. Cell Chem.Biol. 23 415 PMID: 26877022
Truong et al (2022) The fluorescent aptamer Squash extensively repurposes the adenine riboswitch fold. Nat.Chem.Biol. 18 191 PMID: 34937911
Dey et al (2022) Repurposing an adenine riboswitch into a fluorogenic imaging and sensing tag. Nat.Chem.Biol. 18 180 PMID: 34937909
If you know of a relevant reference for DFHBI 1T, please let us know.
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Keywords: DFHBI 1T, DFHBI 1T supplier, DFHBI1T, fluorescence, fluorophores, RNA, imaging, Spinach2, localization, green, fluorescent, protein, Broccoli, light, up, aptamers, FLAPs, Aptamer-based, Imaging, Fluorescent, Probes, 5610, Tocris Bioscience
4 Citations for DFHBI 1T
Citations are publications that use Tocris products. Selected citations for DFHBI 1T include:
Yim et al (2019) Multiplex transcriptional characterizations across diverse bacterial species using cell-free systems. Mol Syst Biol 15 e8875 PMID: 31464371
Paul S et al (2022) Specific Nucleic AcId Ligation for the detection of Schistosomes: SNAILS. PLoS Negl Trop Dis 16 e0010632 PMID: 35881651
Robert et al (2022) Quantitative RNA imaging in single live cells reveals age-dependent asymmetric inheritance. Cell Rep 41 111656 PMID: 36384120
Jeong Wook et al (2020) Cell-free biosensors for rapid detection of water contaminants. Nat Biotechnol 38 1451-1459 PMID: 32632301
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Literature in this Area
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Fluorescent Dyes and Probes Research Product GuideUpdated
This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:
- Fluorescent Dyes, including Janelia Fluor® Dyes
- Fluorescent Probes for Advanced Microscopy and Live Cell Imaging
- Fluorescent Probes to Support In Vivo, Deep Tissue, and Bioluminescence Imaging
- Fluorescent Probes and Reagents for Organoids and 3D Cell Culture Imaging
- Aptamer-based RNA Imaging Reagents
- Fluorescent Probes for Imaging Bacteria
- TSA VividTM Fluorophore Kits
- TSA Reagents for Enhancing IHC, ICC & FISH Signals
- Tissue Clearing Kits and Reagents