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Submit ReviewDFHBI 1T
Description: Mimic of GFP fluorophore for imaging RNA in living cells
Chemical Name: (5Z)-5-[(3,5-Difluoro-4-hydroxyphenyl)methylene]-3,5-dihydro-2-methyl-3-(2,2,2-trifluoroethyl)-4H-imidazol-4-one
Purity: ≥98% (HPLC)
Biological Activity
Mimic of green fluorescent protein (GFP) fluorophore for imaging RNA in living cells. Fluorescence activated by binding to Spinach2 or Broccoli aptamers. Exhibits peak excitation maxima of 482 nm and peak fluorescence emission of 505 nm when bound to Spinach2, enabling imaging with GFP filter cubes. Exhibits higher specific fluorescence and lower background fluorescence when bound to Spinach2 compared with Spinach2-DFHBI.
Technical Data
| M. Wt | 320.21 |
| Formula | C13H9F5N2O2 |
| Storage | Store at -20°C |
| Purity | ≥98% (HPLC) |
| CAS Number | 1539318-36-9 |
| PubChem ID | 101889712 |
| InChI Key | AWYCLBWNRONMQC-WMZJFQQLSA-N |
| Smiles | O=C1N(CC(F)(F)F)C(C)=N\C1=C/C2=CC(F)=C(O)C(F)=C2 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 32.02 | 100 | |
| ethanol | 16.01 | 50 |
Preparing Stock Solutions
The following data is based on the product molecular weight 320.21. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 3.12 mL | 15.61 mL | 31.23 mL |
| 5 mM | 0.62 mL | 3.12 mL | 6.25 mL |
| 10 mM | 0.31 mL | 1.56 mL | 3.12 mL |
| 50 mM | 0.06 mL | 0.31 mL | 0.62 mL |
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Product Datasheets
References
References are publications that support the biological activity of the product.
Song et al (2014) Plug-and-play fluorophores extend the spectral properties of Spinach. J.Am.Chem.Soc. 136 198 PMID: 24393009
Filonov et al (2015) In-gel imaging of RNA processing using broccoli reveals optimal aptamer expression strategies. Chem.Biol. 22 649 PMID: 26000751
Svensen and Jaffrey (2016) Fluorescent RNA Aptamers as a Tool to Study RNA-Modifying Enzymes. Cell Chem.Biol. 23 415 PMID: 26877022
If you know of a relevant reference for DFHBI 1T, please let us know.
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Keywords: DFHBI 1T, DFHBI 1T supplier, DFHBI1T, fluorescence, fluorophores, RNA, imaging, Spinach2, localization, green, fluorescent, protein, Broccoli, Other, Fluorescent, Probes, Aptamers, 5610, Tocris Bioscience
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Anonymous on 10/10/2018
Species: Human
While doing research
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