Home / Product Type / Fluorescence Imaging / Aptamer-based RNA Imaging / DFHBI 1T

DFHBI 1T

Cat. No. 5610 4 Citations Submit a Review Datasheet / COA / SDS
Pricing Availability   Qty
Description: GFP fluorophore mimic for imaging RNA in living cells; activated by binding Spinach2 and Broccoli aptamers
Chemical Name: (5Z)-5-[(3,5-Difluoro-4-hydroxyphenyl)methylene]-3,5-dihydro-2-methyl-3-(2,2,2-trifluoroethyl)-4H-imidazol-4-one
Purity: ≥98% (HPLC)
Datasheet
Citations (4)
Reviews
Literature (1)
Biological Activity Technical Data Solubility Calculators Datasheets References

Biological Activity for DFHBI 1T

DFHBI 1T is a mimic of green fluorescent protein (GFP) fluorophore for imaging RNA in living cells. DFHBI 1T fluorescence is activated by binding to Spinach2 or Broccoli aptamers, also binds to Squash aptamer (Kd = 45 nM). DFHBI 1T exhibits peak excitation maxima of 482 nm and peak fluorescence emission of 505 nm when bound to Spinach2, enabling imaging with GFP filter cubes. Exhibits higher specific fluorescence and lower background fluorescence when bound to Spinach2 compared with Spinach2-DFHBI.

Technical Data for DFHBI 1T

M. Wt 320.21
Formula C13H9F5N2O2
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 1539318-36-9
PubChem ID 101889712
InChI Key AWYCLBWNRONMQC-WMZJFQQLSA-N
Smiles O=C1N(CC(F)(F)F)C(C)=N\C1=C/C2=CC(F)=C(O)C(F)=C2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for DFHBI 1T

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 32.02 100
ethanol 16.01 50

Preparing Stock Solutions for DFHBI 1T

The following data is based on the product molecular weight 320.21. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.12 mL 15.61 mL 31.23 mL
5 mM 0.62 mL 3.12 mL 6.25 mL
10 mM 0.31 mL 1.56 mL 3.12 mL
50 mM 0.06 mL 0.31 mL 0.62 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.
=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Dilution Calculator

Calculate the dilution required to prepare a stock solution.
x
=
x

Product Datasheets for DFHBI 1T

Certificate of Analysis / Product Datasheet
Select another batch:
Safety Datasheet

References for DFHBI 1T

References are publications that support the biological activity of the product.

Song et al (2014) Plug-and-play fluorophores extend the spectral properties of Spinach. J.Am.Chem.Soc. 136 198 PMID: 24393009

Filonov et al (2015) In-gel imaging of RNA processing using broccoli reveals optimal aptamer expression strategies. Chem.Biol. 22 649 PMID: 26000751

Svensen and Jaffrey (2016) Fluorescent RNA Aptamers as a Tool to Study RNA-Modifying Enzymes. Cell Chem.Biol. 23 415 PMID: 26877022

Truong et al (2022) The fluorescent aptamer Squash extensively repurposes the adenine riboswitch fold. Nat.Chem.Biol. 18 191 PMID: 34937911

Dey et al (2022) Repurposing an adenine riboswitch into a fluorogenic imaging and sensing tag. Nat.Chem.Biol. 18 180 PMID: 34937909

If you know of a relevant reference for DFHBI 1T, please let us know.

View Related Products by Target

Keywords: DFHBI 1T, DFHBI 1T supplier, DFHBI1T, fluorescence, fluorophores, RNA, imaging, Spinach2, localization, green, fluorescent, protein, Broccoli, light, up, aptamers, FLAPs, Aptamers, Aptamer-based, Imaging, Fluorescent, Probes, 5610, Tocris Bioscience

4 Citations for DFHBI 1T

Citations are publications that use Tocris products. Selected citations for DFHBI 1T include:

Yim et al (2019) Multiplex transcriptional characterizations across diverse bacterial species using cell-free systems. Mol Syst Biol 15 e8875 PMID: 31464371

Paul S et al (2022) Specific Nucleic AcId Ligation for the detection of Schistosomes: SNAILS. PLoS Negl Trop Dis 16 e0010632 PMID: 35881651

Robert et al (2022) Quantitative RNA imaging in single live cells reveals age-dependent asymmetric inheritance. Cell Rep 41 111656 PMID: 36384120

Jeong Wook et al (2020) Cell-free biosensors for rapid detection of water contaminants. Nat Biotechnol 38 1451-1459 PMID: 32632301

Do you know of a great paper that uses DFHBI 1T from Tocris? Please let us know.

Reviews for DFHBI 1T

There are currently no reviews for this product. Be the first to review DFHBI 1T and earn rewards!

Have you used DFHBI 1T?

Submit a review and receive an Amazon gift card.

$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

Fluorescent Dyes and Probes Research Product Guide

Fluorescent Dyes and Probes Research Product Guide

This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:

  • Fluorescent Dyes, including dyes for flow cytometry
  • Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
  • Tissue Clearing Kits and Reagents
  • Aptamer-based RNA Imaging Reagents
  • Fluorescent Probes for Imaging Bacteria
  • TSA VividTM Fluorophore Kits
  • TSA Reagents for Enhancing IHC, ICC & FISH Signals