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Submit ReviewDFHBI 1T
Description: GFP fluorophore mimic for imaging RNA in living cells; activated by binding Spinach2 and Broccoli aptamers
Chemical Name: (5Z)-5-[(3,5-Difluoro-4-hydroxyphenyl)methylene]-3,5-dihydro-2-methyl-3-(2,2,2-trifluoroethyl)-4H-imidazol-4-one
Purity: ≥98% (HPLC)
Biological Activity for DFHBI 1T
DFHBI 1T is a mimic of green fluorescent protein (GFP) fluorophore for imaging RNA in living cells. Fluorescence activated by binding to Spinach2 or Broccoli aptamers. Exhibits peak excitation maxima of 482 nm and peak fluorescence emission of 505 nm when bound to Spinach2, enabling imaging with GFP filter cubes. Exhibits higher specific fluorescence and lower background fluorescence when bound to Spinach2 compared with Spinach2-DFHBI.
Technical Data for DFHBI 1T
| M. Wt | 320.21 |
| Formula | C13H9F5N2O2 |
| Storage | Store at -20°C |
| Purity | ≥98% (HPLC) |
| CAS Number | 1539318-36-9 |
| PubChem ID | 101889712 |
| InChI Key | AWYCLBWNRONMQC-WMZJFQQLSA-N |
| Smiles | O=C1N(CC(F)(F)F)C(C)=N\C1=C/C2=CC(F)=C(O)C(F)=C2 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for DFHBI 1T
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 32.02 | 100 | |
| ethanol | 16.01 | 50 |
Preparing Stock Solutions for DFHBI 1T
The following data is based on the product molecular weight 320.21. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 3.12 mL | 15.61 mL | 31.23 mL |
| 5 mM | 0.62 mL | 3.12 mL | 6.25 mL |
| 10 mM | 0.31 mL | 1.56 mL | 3.12 mL |
| 50 mM | 0.06 mL | 0.31 mL | 0.62 mL |
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Product Datasheets for DFHBI 1T
References for DFHBI 1T
References are publications that support the biological activity of the product.
Song et al (2014) Plug-and-play fluorophores extend the spectral properties of Spinach. J.Am.Chem.Soc. 136 198 PMID: 24393009
Filonov et al (2015) In-gel imaging of RNA processing using broccoli reveals optimal aptamer expression strategies. Chem.Biol. 22 649 PMID: 26000751
Svensen and Jaffrey (2016) Fluorescent RNA Aptamers as a Tool to Study RNA-Modifying Enzymes. Cell Chem.Biol. 23 415 PMID: 26877022
If you know of a relevant reference for DFHBI 1T, please let us know.
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Keywords: DFHBI 1T, DFHBI 1T supplier, DFHBI1T, fluorescence, fluorophores, RNA, imaging, Spinach2, localization, green, fluorescent, protein, Broccoli, light, up, aptamers, FLAPs, Aptamers, Aptamer-based, Imaging, Fluorescent, Probes, 5610, Tocris Bioscience
1 Citation for DFHBI 1T
Citations are publications that use Tocris products. Selected citations for DFHBI 1T include:
Yim et al (2019) Multiplex transcriptional characterizations across diverse bacterial species using cell-free systems. Mol Syst Biol 15 e8875 PMID: 31464371
Do you know of a great paper that uses DFHBI 1T from Tocris? Please let us know.
Reviews for DFHBI 1T
Average Rating: 4 (Based on 1 Review.)
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Anonymous on 10/10/2018
Species: Human
While doing research
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Fluorescent Probes and Dyes Research Product Guide
This product guide provides a comprehensive list and background to the use of Fluorescent Probes and Dyes
- Fluorescent Dyes
- Dyes for Flow Cytometry
- Fluorescent Probes
- Anti-fade Reagents
- Bioluminescent Substrates
- Fluorogenic Dyes for Light-Up Aptamers
- Fluorescent Probes for Imaging Bacteria
- TSA Reagents for Enhancing IHC, ICC & FISH Signals