DBHDA

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Cat.No. 6175 - DBHDA | C6H10Br2N2O2 | CAS No. 99584-96-0
Description: Reagent for synthetic biology; converts cysteine to dehydroalanine
Chemical Name: 2,5-Dibromohexanediamide
Datasheet
Citations
Literature

Biological Activity

Reagent for synthetic biology; converts cysteine to dehydroalanine (Dha).

Technical Data

M. Wt 301.96
Formula C6H10Br2N2O2
Storage Store at +4°C
CAS Number 99584-96-0
PubChem ID 277410
InChI Key PLSXNAQEJOGNKQ-UHFFFAOYSA-N
Smiles NC(C(CCC(C(N)=O)Br)Br)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 30.2 100

Preparing Stock Solutions

The following data is based on the product molecular weight 301.96. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.31 mL 16.56 mL 33.12 mL
5 mM 0.66 mL 3.31 mL 6.62 mL
10 mM 0.33 mL 1.66 mL 3.31 mL
50 mM 0.07 mL 0.33 mL 0.66 mL

Molarity Calculator

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

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Dilution Calculator

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Product Datasheets

Safety Datasheet

References

References are publications that support the products' biological activity.

Chalker et al (2011) Methods for converting cysteine to dehydroalanine on peptides and proteins. Chem. Sci. 2 1666 PMID:

Wright et al (2016) Posttranslational mutagenesis: a chemical strategy for exploring protein side-chain diversity. Science 354 1465 PMID: 27708059

Mulder et al (2016) A cascading activity-based probe sequentially targets E1-E2-E3 ubiquitin enzymes. Nat.Chem.Biol. 12 523 PMID: 27182664

Lercher et al (2015) Generation of a synthetic GlcNAcylated nucleosome reveals regulation of stability by H2A-Thr101 GlcNAcylation. Nat.Commun. 25 7978 PMID: 26305776


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