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Biological Activity for DBHDA
DBHDA is a reagent for synthetic biology; converts cysteine to dehydroalanine (Dha).
Technical Data for DBHDA
|Storage||Store at +4°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for DBHDA
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions for DBHDA
The following data is based on the product molecular weight 301.96. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||3.31 mL||16.56 mL||33.12 mL|
|5 mM||0.66 mL||3.31 mL||6.62 mL|
|10 mM||0.33 mL||1.66 mL||3.31 mL|
|50 mM||0.07 mL||0.33 mL||0.66 mL|
References for DBHDA
References are publications that support the biological activity of the product.
Chalker et al (2011) Methods for converting cysteine to dehydroalanine on peptides and proteins. Chem. Sci. 2 1666
Wright et al (2016) Posttranslational mutagenesis: a chemical strategy for exploring protein side-chain diversity. Science 354 1465 PMID: 27708059
Mulder et al (2016) A cascading activity-based probe sequentially targets E1-E2-E3 ubiquitin enzymes. Nat.Chem.Biol. 12 523 PMID: 27182664
Lercher et al (2015) Generation of a synthetic GlcNAcylated nucleosome reveals regulation of stability by H2A-Thr101 GlcNAcylation. Nat.Commun. 25 7978 PMID: 26305776
If you know of a relevant reference for DBHDA, please let us know.
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Citations for DBHDA
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