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DBCO-PEG4-Biotin is a biotinylation reagent for labeling azide-containing biomolecules via Cu(I)-free Strain-Promoted Alkyne-Azide Click Chemistry (SPAAC) reaction. The hydrophilic PEG4 linker reduces or eliminates aggregation and precipitation during the labeling process by increasing the hydrophilicity of the target molecule. The PEG linker in DBCO-PEG4-Biotin also enhances the accessibility of the biotin moiety, improving the detection efficiency of the biotinylated molecule via fluorescent or HRP-labeled streptavidin or its affinity purification via streptavidin agarose.
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
The following data is based on the product molecular weight 749.92. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||1.33 mL||6.67 mL||13.33 mL|
|5 mM||0.27 mL||1.33 mL||2.67 mL|
|10 mM||0.13 mL||0.67 mL||1.33 mL|
|50 mM||0.03 mL||0.13 mL||0.27 mL|
References are publications that support the biological activity of the product.
Agard et al (2006) A comparative study of bioorthogonal reactions with azides. ACS Chem.Biol. 1 644 PMID: 17175580
Bernardim et al (2020) Precise installation of diazo-tagged side-chains on proteins to enable in vitro and in-cell site-specific labeling. Bioconjug.Chem. 31 1604 PMID: 32375474
Darabedian et al (2020) O-Acetylated chemical reporters of glycosylation can display metabolism-dependent background labeling of proteins but are generally reliable tools for the identification of glycoproteins. Front.Chem. 8 318 PMID: 32411667
Sawant et al (2016) A versatile toolbox for posttranscriptional chemical labeling and imaging of RNA. Nucleic Acids Res. 44 e16 PMID: 26384420
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Keywords: DBCO PEG4 Biotin, DBCO PEG4 Biotin supplier, Cu(I)-free, Strain-Promoted, Alkyne-Azide, Click, Chemistry, SPAAC, copper-free, biotinylation, labeling, labelling, HRP, horseradish, peroxidase, streptavidin, fluorescent, fluorescence, imaging, Biochemicals, and, Molecular, Biology, 7480, Tocris Bioscience
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