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D-Luciferin sodium salt
Firefly luciferase substrate (Km = ~2 μM). Used in many standard bioluminescence imaging (BLI) techniques, including monitoring tumour growth, and investigating intracellular signaling activity in vitro and in vivo. Cell permeable. Emission maximum = 562 nm.
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions
The following data is based on the product molecular weight 302.3. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||3.31 mL||16.54 mL||33.08 mL|
|5 mM||0.66 mL||3.31 mL||6.62 mL|
|10 mM||0.33 mL||1.65 mL||3.31 mL|
|50 mM||0.07 mL||0.33 mL||0.66 mL|
References are publications that support the biological activity of the product.
Keyaerts et al (2008) Dynamic bioluminescence imaging for quantitative tumour burden assessment using IV or IP administration of D: -luciferin: effect on intensity, time kinetics and repeatability of photon emission. Eur.J.Nucl.Med.Mol.Imaging. 35 999 PMID: 18180921
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