Compound 401

Pricing Availability Delivery Time Qty
Cat.No. 3271 - Compound 401 | C16H15N3O2 | CAS No. 168425-64-7
Description: Selective DNA-PK and mTOR inhibitor
Chemical Name: 2-(4-Morpholinyl)-4H-pyrimido[2,1-a]isoquinolin-4-one
Purity: ≥99% (HPLC)
Datasheet
Citations
Literature

Biological Activity

Reversible and selective inhibitor of DNA-dependent protein kinase (DNA-PK) and mammalian target of rapamycin (mTOR) (IC50 values are 0.28 and 5.3 μM respectively). Displays little affinity for other commonly studied kinases including PI 3-K, ATM and ATR (IC50 values are all > 100 μM). Induces apoptosis in vitro.

Technical Data

M. Wt 281.31
Formula C16H15N3O2
Storage Store at RT
Purity ≥99% (HPLC)
CAS Number 168425-64-7
PubChem ID 10039361
InChI Key BVRDQVRQVGRNHG-UHFFFAOYSA-N
Smiles O=C3N2C=CC1=CC=CC=C1C2=NC(N4CCOCC4)=C3

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 2.81 10
ethanol 1.41 5

Preparing Stock Solutions

The following data is based on the product molecular weight 281.31. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.55 mL 17.77 mL 35.55 mL
5 mM 0.71 mL 3.55 mL 7.11 mL
10 mM 0.36 mL 1.78 mL 3.55 mL
50 mM 0.07 mL 0.36 mL 0.71 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.
=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Dilution Calculator

Calculate the dilution required to prepare a stock solution.
x
=
x

Product Datasheets

Safety Datasheet

References

References are publications that support the products' biological activity.

Griffen et al (2005) Selective benzopyranone and pyrimido[2,1-α]isoquinolin-4-one inhibitors of DNA-dependent protein kinase: synthesis, structure-activity studies and radiosensitization of a human tumor cell line in vitro. J.Med.Chem. 48 569 PMID: 15658870

Ballo et al (2007) Inhibition of mammalian target of rapamycin signaling by 2-(morpholin-1-yl)oyrimido[2,1-α]isoquinolin-4-one. J.Biol.Chem. 282 24463 PMID: 17562705


If you know of a relevant reference for Compound 401, please let us know.

View Related Products by Product Action

View all DNA-Dependent Protein Kinase Inhibitors

Keywords: Selective DNA-PK mTOR inhibitors inhibits Checkpoint Control Kinases DNA-Dependent Protein Kinase PI3K Phosphoinositide PI 3-kinase Akt Signaling Signalling Mammalian Target Rapamycin FRAP Compound401 DNA-dependent Protein Kinase

Citations for Compound 401

Citations are publications that use Tocris products.

Currently there are no citations for Compound 401. Do you know of a great paper that uses Compound 401 from Tocris? If so please let us know.

Commented out for usability testing

Reviews

TODO: Add Reviews

Literature in this Area

Cancer

Cancer Research Product Guide

A collection of over 750 products for cancer research, the guide includes research tools for the study of:

  • Cancer Metabolism
  • Epigenetics in Cancer
  • Receptor Signaling
  • Cell Cycle and DNA Damage Repair
  • Angiogenesis
  • Invasion and Metastasis
Kinases

Kinases Product Listing

A collection of over 400 products for kinase research, the listing includes inhibitors of:

  • Receptor Tyrosine Kinases
  • Protein Kinases A, C, D and G
  • PI-3 Kinase, Akt and mTOR
  • MAPK Signaling
  • Receptor Serine/Threonine Kinases
Cell Cycle & DNA Damage Repair

Cell Cycle & DNA Damage Repair Poster

In normal cells, each stage of the cell cycle is tightly regulated, however in cancer cells many genes and proteins that are involved in the regulation of the cell cycle are mutated or over expressed. Adapted from the 2015 Cancer Product Guide, Edition 3, this poster summarizes the stages of the cell cycle and DNA repair. It also highlights strategies for enhancing replicative stress in cancer cells to force mitotic catastrophe and cell death.

Pathways for Compound 401

Protocols

TODO: Add Protocols