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Submit ReviewClariTSA™ Fluorophore Kit 520
Description: Signal amplification kit for use in ISH, ICC and IHC
Biological Activity for ClariTSA™ Fluorophore Kit 520
Please note: this product was formerly known as TSA Vivid Fluorophore Kit 520.
ClariTSA™ reagents are exceptionally bright dyes that offer an effective way to boost signal intensity and detect low-abundance targets in spatial biology applications. They are designed for use in ISH, IHC and ICC experiments where in situ detection of target protein or nucleic acid sequences is required. ClariTSA reagents have been specifically optimized to deliver exceptional signal-to-noise in the RNAscope™ Multiplex Fluorescent v2 Assay for visualization of gene expression at the single cell level. They are suitable for multiplexing and can be combined with DAPI (Cat. No. 5748) counter-staining.
The basis for signal amplification comes from the specific and high-density deposition of ClariTSA dyes adjacent to a horseradish peroxidase (HRP)-labeled probe in a process termed Tyramide Signal Amplification (TSA) or Catalyzed Reporter Deposition (CARD).
Excitation maximum = 494 nm; emission maximum = 517 nm
Please see the product datasheet or protocol for information on product use.
ClariTSA™ is a trademark of Bio-Techne Corp.
Scientific Data
Application of ClariTSA™ Fluorophore Kit 520 in RNAscope Multiplex Fluorescent v2 Assay RNAscope™ Multiplex Fluorescent v2 Assay on HeLa cells with the leading competitor dye (left) and ClariTSA™ Fluorophore Kit 520 dye (right). Dye used at 1:1500 dilution. Marker shown is Polr2a in green. Nuclei are counterstained with DAPI (blue, Cat. No. 5748).
Optical Data for ClariTSA™ Fluorophore Kit 520
 | Emission Color | Green |
| λabs | 494 nm | |
| λem | 517 nm |
Technical Data for ClariTSA™ Fluorophore Kit 520
| Storage | Store at -20°C |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Product Datasheets for ClariTSA™ Fluorophore Kit 520
References for ClariTSA™ Fluorophore Kit 520
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Fluorescent Dyes and Probes Research Product Guide
This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:
- Fluorescent Dyes, including Janelia Fluor® Dyes
- Fluorescent Probes for Advanced Microscopy and Live Cell Imaging
- Fluorescent Probes to Support In Vivo, Deep Tissue, and Bioluminescence Imaging
- Fluorescent Probes and Reagents for Organoids and 3D Cell Culture Imaging
- Aptamer-based RNA Imaging Reagents
- Fluorescent Probes for Imaging Bacteria
- TSA VividTM Fluorophore Kits
- TSA Reagents for Enhancing IHC, ICC & FISH Signals
- Tissue Clearing Kits and Reagents