Cell Activation Cocktail 500X

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Cat.No. 5476 - Cell Activation Cocktail 500X
Description: Cell activation cocktail
Reviews (2)

Biological Activity for Cell Activation Cocktail 500X

Cell Activation Cocktail 500X contains the following compounds: Monensin sodium salt (1.5 mM), Phorbol 12-myristate 13-acetate (0.0405 mM) and Ionomycin calcium salt (0.67 mM). Cocktail is provided as 1 ml of 500X concentrate in ethanol.

Technical Data for Cell Activation Cocktail 500X

Storage Store at -80°C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Cell Activation Cocktail 500X

Solubility Soluble in ethanol (supplied pre-dissolved in 1ml ethanol)

Product Datasheets for Cell Activation Cocktail 500X

References for Cell Activation Cocktail 500X

References are publications that support the biological activity of the product.

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Keywords: Cell Activation Cocktail 500X, Cell Activation Cocktail 500X supplier, Monensin, Phorbol, 12-myristate, 13-acetate, PMA, Ionomycin, Biochemicals, and, Molecular, Biology, 5476, Tocris Bioscience

Citations for Cell Activation Cocktail 500X

Citations are publications that use Tocris products.

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Reviews for Cell Activation Cocktail 500X

Average Rating: 5 (Based on 2 Reviews.)

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Cell Activation Cocktail.
By Anonymous on 10/09/2018
Assay Type: In Vitro
Species: Mouse
Cell Line/Tissue: splenocytes/ T cells

This product was used to perform a TH17 differentiation assay using a flow cytometry analysis afterwards. This cocktail has made cell activation easier and my experimental preparation move along much faster.

Used to stimulate tumor infiltrating lymphocytes after isolation.
By Anonymous on 02/22/2018
Assay Type: In Vitro
Species: Mouse
Cell Line/Tissue: T cells

Tumor infiltrating lymphocytes (TILs) were isolated from mouse liver tumors and then stimulated with 1X cell activation cocktail (diluted in RPMI media). After 5 hour activation, cytokine production on the TILs was measured via flow cytometry. Example picture shows CD107A expression before and after activation.

Always use it at 1X.