Potent, selective and photo-dependent inhibitor of protein kinase C that targets the regulatory domain (IC50 = 50 nM). Displays > 1000-fold selectivity over other protein kinases such as cAMP-dependent protein kinase and tyrosine-specific protein kinase. Inhibits cell proliferation of malignant glioma cells in light-treated conditions in vitro (IC50 ~ 40 - 60 nM). Also an antagonist of the Tcf/β-catenin complex.
|Storage||Desiccate at +4°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
|Solubility||Soluble in DMSO and in ethanol|
Preparing Stock Solutions
The following data is based on the product molecular weight 790.76. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||1.26 mL||6.32 mL||12.65 mL|
|5 mM||0.25 mL||1.26 mL||2.53 mL|
|10 mM||0.13 mL||0.63 mL||1.26 mL|
|50 mM||0.03 mL||0.13 mL||0.25 mL|
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.
References are publications that support the products' biological activity.
Pollack and Kawecki (1997) The effect of calphostin C, a potent photodependent protein kinase C inhibitor, on the proliferation of glioma cells in vitro. J.Neurooncol. 31 255 PMID: 9049854
Au et al (2006) Differential effects of photofrin, 5-aminolevulinic acid and calphostin C on glioma cells. J.Photochem.Photobiol.B. 85 92 PMID: 16829117
Matsuzaki and Darcha (2013) In vitro effects of a small-molecule antagonist of the Tcf/β-catenin complex on endometrial and endometriotic cells of patients with endometriosis. PLoS One 8 e61690 PMID: 23626717
If you know of a relevant reference for Calphostin C, please let us know.
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Keywords: Potent selective photo-dependent PKC inhibitors inhibits Protein Kinases C CalphostinC UCN1028C PKF115-584 stem cells UCN 1028C PKF 115584 Protein Kinase C
4 Citations for Calphostin C
Citations are publications that use Tocris products. Selected citations for Calphostin C include:
Cao et al (2015) A novel mechanism for cytoprotection against hypoxic injury: δ-opioid receptor-mediated increase in Nrf2 translocation. PLoS One 172 1869 PMID: 25439010
Merino et al (2015) Glucagon Increases Beating Rate but Not Contractility in Rat Right Atrium. Comparison with Isoproterenol. Am J Physiol Regul Integr Comp Physiol 10 e0132884 PMID: 26222156
Liang et al (2014) δ-Opioid receptors up-regulate excitatory amino acid transporters in mouse astrocytes. Br J Pharmacol 171 5417 PMID: 25052197
Most et al (2013) S100A1 deficiency impairs postischemic angiogenesis via compromised proangiogenic endothelial cell function and nitric oxide synthase regulation. Circ Res 112 66 PMID: 23048072
Do you know of a great paper that uses Calphostin C from Tocris? If so please let us know.