Pricing Availability   Qty
Description: Calpain and cathepsin L inhibitor
Chemical Name: N-Benzyloxycarbonyl-L-leucylnorleucinal
Citations (10)

Biological Activity for Calpeptin

Calpeptin is a potent, cell-permeable inhibitor of the Ca2+-dependent protease, calpain. Prevents collagen- and thrombin-induced platelet aggregation, probably by blocking calpain induced phospholipase C and thromboxane synthase activation. Potent cathepsin L inhibitor. Recently shown to preferentially inhibit a subset of protein-tyrosine phosphatases. Also inhibits SARS-CoV-2 Mpro in vitro (IC50 = 10.7 μM) and inhibits viral replication.

Technical Data for Calpeptin

M. Wt 362.47
Formula C20H30N2O4
Storage Desiccate at +4°C
CAS Number 117591-20-5
PubChem ID 73364
Smiles O=C(OCC1=CC=CC=C1)N[C@H](C(N[C@H](C([H])=O)CCCC)=O)CC(C)C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Calpeptin

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 100

Preparing Stock Solutions for Calpeptin

The following data is based on the product molecular weight 362.47. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.76 mL 13.79 mL 27.59 mL
5 mM 0.55 mL 2.76 mL 5.52 mL
10 mM 0.28 mL 1.38 mL 2.76 mL
50 mM 0.06 mL 0.28 mL 0.55 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Product Datasheets for Calpeptin

Certificate of Analysis / Product Datasheet
Select another batch:

References for Calpeptin

References are publications that support the biological activity of the product.

Mehdi (1991) Cell-penetrating inhibitors of calpain. TiBS 16 150 PMID: 1877091

Sasaki et al (1990) Inhibitory effect of di-and tripeptidyl aldehydes on calpains and cathepsins. J.Enzyme Inhib. 3 195 PMID: 2079636

Schoenwaelder and Burridge (1999) Evidence for a calpeptin-sensitive protein-tyrosine phosphatase upstream of the small GTPase Rho. J.Biol.Chem. 274 14359 PMID: 10318859

Tsujinaka et al (1988) Synthesis of a new cell penetrating calpain inhibitor (calpeptin). Biochem.Biophys.Res.Commun. 153 1201 PMID: 2839170

Ma et al (2020) Boceprevir, GC-376, and calpain inhibitors II, XII inhibit SARS-CoV-2 viral replication by targeting the viral main protease. Cell Res. 30 678 PMID: 32541865

If you know of a relevant reference for Calpeptin, please let us know.

View Related Products by Product Action

View all Calcium-Sensitive Protease Inhibitors

Keywords: Calpeptin, Calpeptin supplier, Calpain, cathepsin, L, inhibitors, inhibits, Proteinases, Proteases, Ca2+Sensitive, Protease, modulators, Calcium-Sensitive, Ca2+, Signaling, Signalling, Calpains, Cathepsin, 3C, and, 3CL, 0448, Tocris Bioscience

10 Citations for Calpeptin

Citations are publications that use Tocris products. Selected citations for Calpeptin include:

Zakharova et al (2015) Platelet surface-associated activation and secretion-mediated inhibition of coagulation factor XII. PLoS One 10 e0116665 PMID: 25688860

Amadoro et al (2006) NMDA receptor mediates tau-induced neurotoxicity by calpain and ERK/MAPK activation. Proc Natl Acad Sci U S A 103 2892 PMID: 16477009

Zhou and Cai (2019) Calpeptin Reduces Neurobehavioral Deficits and Neuronal Apoptosis Following Subarachnoid Hemorrhage in Rats. J Stroke Cerebrovasc Dis 28 125 PMID: 30337208

Leytin et al (2009) Mitochondrial control of platelet apoptosis: effect of cyclosporin A, an inhibitor of the mitochondrial permeability transition pore. Lab Invest 89 374 PMID: 19238135

Do you know of a great paper that uses Calpeptin from Tocris? Please let us know.

Reviews for Calpeptin

There are currently no reviews for this product. Be the first to review Calpeptin and earn rewards!

Have you used Calpeptin?

Submit a review and receive an Amazon gift card.

$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review