Caffeine Orange

Pricing Availability   Qty
Description: Selective, BODIPY-based fluorescent caffeine chemosensor
Chemical Name: (E)-5,5-Difluoro-3-(2-(5-fluoro-1H-indol-3-yl)vinyl)-1-methyl-5H-5λ4,6λ4-dipyrrolo[1,2-c:2',1'-f][1,3,2]diazaborinine
Purity: ≥95% (HPLC)
Literature (1)

Biological Activity for Caffeine Orange

Caffeine Orange is a selective, BODIPY-based fluorescent caffeine chemosensor, which exhibits up to 250-fold enhancement in its fluorescence upon caffeine activation (KD = 16.81 mM and detection limit of 50 µM). Caffeine Orange works both in aqueous and in dry state, and its red emission intensity increases with increasing caffeine concentrations. Excitation maximum ~580 nM; emission maximum ~629 nM.

Optical Data for Caffeine Orange

Caffeine Orange Dye Spectra
Emission Color Orange
λabs 582 nm
λem 618 nm
Extinction Coefficient (ε) 70000 M-1cm-1
Quantum Yield (φ) 0.28
Application Fluorescence Microscopy

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectral Viewer

Spectral Viewer

Technical Data for Caffeine Orange

M. Wt 365.17
Formula C20H15BF3N3
Storage Store at -20°C
Purity ≥95% (HPLC)
CAS Number 1632296-13-9
PubChem ID 86280912
Smiles CC1=C2C=C3C=CC=[N+]3[B-](F)(N2C(/C=C/C4=CNC5=CC=C(C=C45)F)=C1)F

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Caffeine Orange

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 3.65 10

Preparing Stock Solutions for Caffeine Orange

The following data is based on the product molecular weight 365.17. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.1 mM 27.38 mL 136.92 mL 273.85 mL
0.5 mM 5.48 mL 27.38 mL 54.77 mL
1 mM 2.74 mL 13.69 mL 27.38 mL
5 mM 0.55 mL 2.74 mL 5.48 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Product Datasheets for Caffeine Orange

References for Caffeine Orange

References are publications that support the biological activity of the product.

Xu et al (2013) Make caffeine visible: a fluorescent caffeine "traffic light" detector Sci.Rep. 3 2255 PMID: 23877095

If you know of a relevant reference for Caffeine Orange, please let us know.

Keywords: Caffeine Orange, Caffeine Orange supplier, selective, fluorescent, caffeine, chemosensor, BODIPY, Other, Fluorescent, Probes, 7181, Tocris Bioscience

Citations for Caffeine Orange

Citations are publications that use Tocris products.

Currently there are no citations for Caffeine Orange. Do you know of a great paper that uses Caffeine Orange from Tocris? Please let us know.

Reviews for Caffeine Orange

There are currently no reviews for this product. Be the first to review Caffeine Orange and earn rewards!

Have you used Caffeine Orange?

Submit a review and receive an Amazon gift card.

$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

Fluorescent Dyes and Probes Research Product Guide

Fluorescent Dyes and Probes Research Product Guide

This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:

  • Fluorescent Dyes, including dyes for flow cytometry
  • Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
  • Tissue Clearing Kits and Reagents
  • Aptamer-based RNA Imaging Reagents
  • Fluorescent Probes for Imaging Bacteria
  • TSA VividTM Fluorophore Kits
  • TSA Reagents for Enhancing IHC, ICC & FISH Signals