Pricing Availability   Qty
Description: TRAF6-Ubc13 interaction inhibitor
Chemical Name: 3-[3,5-Dimethyl-1-(3-methyl-1,2,4-triazolo[4,3-b]pyridazin-6-yl)-1H-pyrazol-4-yl]-1-[4-(phenylmethyl)-1-piperidinyl]-1-propanone
Purity: ≥98% (HPLC)
Literature (3)

Biological Activity for C25-140

C25-140 binds directly to TNF receptor-associated factor 6 (TRAF6) and inhibits its interaction with ubiquitin-conjugating enzyme Ubc13. C25-140 reduces TRAF6-Ubc13 activity and decreases NF-κB activation and inflammatory signaling in vitro. C25-140 exhibits selectivity for TRAF6 over other E3 ligases except cIAP1. In in vivo mouse models of autoimmune psoriasis and rheumatoid arthritis, the compound improves disease symptoms. The compound also suppresses the pro-inflammatory signaling activity of IL-17A and prevents its inhibitory effect on the anti-HBV action of IFN-α HepG2 cells.

Technical Data for C25-140

M. Wt 457.57
Formula C26H31N7O
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 1358099-18-9
PubChem ID 53093165
Smiles O=C(N1CCC(CC=2C=CC=CC2)CC1)CCC=3C(=NN(C4=NN5C(=NN=C5C)C=C4)C3C)C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for C25-140

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 22.88 50
ethanol 4.58 10

Preparing Stock Solutions for C25-140

The following data is based on the product molecular weight 457.57. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.5 mM 4.37 mL 21.85 mL 43.71 mL
2.5 mM 0.87 mL 4.37 mL 8.74 mL
5 mM 0.44 mL 2.19 mL 4.37 mL
25 mM 0.09 mL 0.44 mL 0.87 mL

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References for C25-140

References are publications that support the biological activity of the product.

Brenke et al (2018) Targeting TRAF6 E3 ligase activity with a small-molecule inhibitor combats autoimmunity. J.Biol.Chem. 293 13191 PMID: 29950522

Zhang et al (2022) Interleukin-17A pretreatment attenuates the anti-hepatitis B virus efficacy of interferon-alpha by reducing activation of the interferon-stimulated gene factor 3 transcriptional complex in hepatitis B virus-expressing HepG2 cells. Virol.J. 19 28 PMID: 35144643

If you know of a relevant reference for C25-140, please let us know.

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Keywords: C25-140, C25-140 supplier, inhibits, inhibitors, TRAF6-Ubc13, NF-kB, NF-KappaB, κ, E3, ligases, Ubiquitin, Ligases, NF-kB/IkB, 7715, Tocris Bioscience

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