BTT 3033

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Description: Selective inhibitor of integrin α2β1
Chemical Name: 1-(4-Fluorophenyl)-N-methyl-N-[4[[(phenylamino)carbonyl]amino]phenyl]-1H-pyrazole-4-sulfonamide
Purity: ≥98% (HPLC)
Citations (4)
Reviews (1)
Literature (2)

Biological Activity for BTT 3033

BTT 3033 is a selective inhibitor of integrin α2β1 (EC50 = 130 nM for α2β1 binding to collagen I). Binds to the α2I domain. Exhibits selectivity for α2β1 over integrins α3β1, α4β1, α5β1 and αv. BTT 3033 inhibits platelet aggregation to collagen I coated capillaries under flow and inhibits binding of α2-expressing CHO cells to collagen I under shear stress conditions. Neurogenic and thromboxane A2-induced human prostate smooth muscle contraction are also inhibited by BTT 3033.

Compound Libraries for BTT 3033

BTT 3033 is also offered as part of the Tocriscreen 2.0 Max. Find out more about compound libraries available from Tocris.

Technical Data for BTT 3033

M. Wt 465.5
Formula C23H20FN5O3S
Storage Store at +4°C
Purity ≥98% (HPLC)
CAS Number 1259028-99-3
PubChem ID 49841251
Smiles FC1=CC=C(N2N=CC(S(N(C3=CC=C(NC(NC4=CC=CC=C4)=O)C=C3)C)(=O)=O)=C2)C=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for BTT 3033

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 46.55 100

Preparing Stock Solutions for BTT 3033

The following data is based on the product molecular weight 465.5. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.15 mL 10.74 mL 21.48 mL
5 mM 0.43 mL 2.15 mL 4.3 mL
10 mM 0.21 mL 1.07 mL 2.15 mL
50 mM 0.04 mL 0.21 mL 0.43 mL

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References for BTT 3033

References are publications that support the biological activity of the product.

Nissinen et al (2012) Novel a2 beta 1 integrin inhibitors reveal that integrin binding to collagen under shear stress conditions does not require receptor preactivation. J.Cell Biol. 287 44694 PMID: 23132859

Li et al (2021) Phosphoproteomics identifies potential downstream targets of the integrin α2β1 inhibitor BTT-3033 in prostate stromal cells. Ann.Transl.Med. 9 1380 PMID: 34733932

If you know of a relevant reference for BTT 3033, please let us know.

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View all Integrin Inhibitors

Keywords: BTT 3033, BTT 3033 supplier, BTT3033, selective, inhibitors, inhibits, integrins, receptors, alpha2beta1, α2β1, a2b1, collagen, 1, cell, adhesion, Integrins, Cell, Adhesion, Molecules, 4724, Tocris Bioscience

4 Citations for BTT 3033

Citations are publications that use Tocris products. Selected citations for BTT 3033 include:

Gertraud et al (2023) How the mechanobiology orchestrates the iterative and reciprocal ECM-cell cross-talk that drives microtissue growth. Sci Adv 9 eadd9275 PMID: 36989370

Li et al (2021) Phosphoproteomics identifies potential downstream targets of the integrin α2β1 inhibitor BTT-3033 in prostate stromal cells. Ann.Transl.Med. 9 1380 PMID: 34733932

Elaine T et al (2021) Collagen I Fibrous Substrates Modulate the Proliferation and Secretome of Estrogen Receptor-Positive Breast Tumor Cells in a Hormone-Restricted Microenvironment. ACS Biomater Sci Eng 7 2430-2443 PMID: 33688723

Mogami (2018) Collagen Type 1 accelerates healing of ruptured fetal membranes. Sci Rep 8 696 PMID: 29330408

Do you know of a great paper that uses BTT 3033 from Tocris? Please let us know.

Reviews for BTT 3033

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Collagen type 1 activated collagen receptor discoidin domain receptor.
By Anonymous on 02/21/2020
Assay Type: In Vitro
Species: Mouse
Cell Line/Tissue: 3D Culture Matrix

10 μg/ml treat 24h and 48h

PMID: 29330408
review image

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

Angiogenesis in Cancer Poster

Angiogenesis in Cancer Poster

This poster summarizes the pathogenesis of angiogenesis in cancer, as well as some of the main angiogenesis therapeutic targets.

Enabling Research By Provision of Chemical Tools Poster

Enabling Research By Provision of Chemical Tools Poster

The Tocris chemistry team has considerable chemistry knowledge and skill which it has recently been applying to the generation of new chemical probes from known tools and compounds to help answer biological questions. This poster presents some of the work carried out by Tocris scientists and focuses on two areas: the generation of libraries of chemical building blocks to support Degrader (PROTAC) research and the development of a fluorescent probe to study integrin biology. Presented at Chemical Tools for Complex Biological Systems II, 2019, Janelia Research Campus, USA.